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Article

In-Vitro Plant Growth Promotion of Rhizobium Strains Isolated from Lentil Root Nodules under Abiotic Stresses

1
Rhizobium Laboratory, Genetic Resources Section, ICARDA (International Center for Agricultural Research in the Dry Area), Agdal, Rabat 10080, Morocco
2
Microbiology and Molecular Biology Laboratory, Biology Department, Faculty of Sciences-Mohammed V University, Rabat 1014, Morocco
3
Virology, Microbiology, Quality/Eco-Toxicology and Biodiversity Laboratory, Faculty of Sciences and Techniques of Mohammedia, Hassan II University, Mohammedia-Casablanca 146, Morocco
4
School Normale Superieure, Mohamed V University, Rabat 5118, Morocco
*
Author to whom correspondence should be addressed.
Agronomy 2020, 10(7), 1006; https://doi.org/10.3390/agronomy10071006
Received: 2 June 2020 / Revised: 30 June 2020 / Accepted: 3 July 2020 / Published: 13 July 2020
(This article belongs to the Special Issue Mechanism of Rhizosphere Microorganisms Promoting Crop Growth)
Plant growth-promoting rhizobia are known to improve crop performance by multiple mechanisms. However, the interaction between host plants and Rhizobium strains is highly influenced by growing conditions, e.g., heat, cold, drought, soil salinity, nutrient scarcity, etc. The present study was undertaken to assess the use of Rhizobium as plant growth promoters under abiotic stress conditions. Fifteen Rhizobium strains isolated from lentil root nodules were tested for phosphate solubilization activity (PSA) and phytohormones production under salt and drought conditions. The results showed that 15 Rhizobium strains were significant phosphate solubilizers, and indole acedic acid (IAA) and gibberellic acid (GA3) producers based on least significant difference (LSD) analysis (p ≤ 0.05). The highest rate of PSA was attributed to three strains namely, 1145N5, 1159N11, and 1159N32 with a range of 144.6 to 205.6 P2O5 (µg/mL). The highest IAA production was recorded in the strain 686N5 with 57.68 ± 4.25 µg/mL as compared to 50.8667 ± 1.41 µg/mL and 37.32 ± 12.59 µg/mL for Rhizobium tropici CIAT 899 and Azospirillum brasilense DSM-1690, respectively. Strain 318N2111 produced 329.24 ± 7.84 µg/mL of GA3 as against 259.84 ± 25.55 µg/mL for A. brasilense DSM-1690. R. tropici CIAT 899 showed tolerance to salt (5% NaCl) and drought (ψ = −2.6 MPa) stress, whereas strain 686N5 showed an extremely high level of salt-tolerance (5% NaCl) and moderate level of drought tolerance (ψ = −0.75 MPa). These results indicate different pathways for drought and salt tolerance mechanisms. The assessment of plant growth promoting (PGP) activities of Rhizobium showed differences between bacterial viability and bacterial PGP activity in terms of abiotic stress tolerance where bacterial PGP activity is interrupted before reaching the bacterial tolerance threshold. These results integrate a new concept of PGPR screening based on PGP activity under abiotic stress. View Full-Text
Keywords: Rhizobia; salt stress; drought stress; phosphate solubilization; Indole Acetic acid; Plant Growth Promotion Rhizobia; salt stress; drought stress; phosphate solubilization; Indole Acetic acid; Plant Growth Promotion
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MDPI and ACS Style

Sijilmassi, B.; Filali-Maltouf, A.; Fahde, S.; Ennahli, Y.; Boughribil, S.; Kumar, S.; Amri, A. In-Vitro Plant Growth Promotion of Rhizobium Strains Isolated from Lentil Root Nodules under Abiotic Stresses. Agronomy 2020, 10, 1006. https://doi.org/10.3390/agronomy10071006

AMA Style

Sijilmassi B, Filali-Maltouf A, Fahde S, Ennahli Y, Boughribil S, Kumar S, Amri A. In-Vitro Plant Growth Promotion of Rhizobium Strains Isolated from Lentil Root Nodules under Abiotic Stresses. Agronomy. 2020; 10(7):1006. https://doi.org/10.3390/agronomy10071006

Chicago/Turabian Style

Sijilmassi, Badreddine; Filali-Maltouf, Abdelkarim; Fahde, Sara; Ennahli, Youness; Boughribil, Said; Kumar, Shiv; Amri, Ahmed. 2020. "In-Vitro Plant Growth Promotion of Rhizobium Strains Isolated from Lentil Root Nodules under Abiotic Stresses" Agronomy 10, no. 7: 1006. https://doi.org/10.3390/agronomy10071006

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