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Open AccessArticle

Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive Substances

1
Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nábrežie mládeže 91, 949 74 Nitra, Slovakia
2
Department of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, 760 01 Zlín, Czech Republic
3
Institute for Teacher Training, Faculty of Central European Studies, Constantine the Philosopher University in Nitra, Dražovská 4, 949 74 Nitra, Slovakia
4
Department of Genetics and Plant Breeding, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia
5
Horticulture and Landscape Engineering Faculty, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
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Department of Breeding and Propagation of Horticultural Plants, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech Republic
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Department of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nábrežie mládeže 91, 949 74 Nitra, Slovakia
*
Author to whom correspondence should be addressed.
Agronomy 2020, 10(6), 868; https://doi.org/10.3390/agronomy10060868
Received: 21 May 2020 / Revised: 16 June 2020 / Accepted: 16 June 2020 / Published: 18 June 2020
(This article belongs to the Special Issue Use of Wild Crop Relatives as Genetic Resources in Crop Breeding)
This study aimed to analyze the genetic diversity of selected cultivars and clones of Lonicera sp. (edible honeysuckle) from two countries with different climatic and soil conditions. Accordingly, the determination of total polyphenols content (TPC) and total antioxidant activity (TAA) in fruits of Lonicera sp. in 2018–2019 was performed. Applied statistical methods evaluating TPC and TAA were: correlation and regression, ANOVA, and agglomerative hierarchical clustering. The neighbor-joining method and gel images analysis (PyElph software, Pavel and Vasile, Bucharest, Romania) were used for genetic analysis. The average TPC of all cultivars and clones was 5.32 mg L−1 in the year 2018 and 3.78 mg L−1 in 2019. The average TAA values of all samples in 2018 were 8.24 mg L−1, and 9.27 mg L−1 in 2019. Statistically significant correlations between years and cultivars and clones were found. Based on the lengths and number of fragments by random amplified polymorphic DNA (RAPD)-PCR’s, it was found that ‘clone 5’ and ‘clone 7’ were genetically close to the grown cultivars from the Czech Republic. Therefore, edible honeysuckle fruits are a valuable source of important nutrients for the human body and its health. View Full-Text
Keywords: honeysuckle; DNA polymorphism; TAA; TPC honeysuckle; DNA polymorphism; TAA; TPC
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MDPI and ACS Style

Cehula, M.; Mlček, J.; Juríková, T.; Žiarovská, J.; Paulen, O.; Dokoupil, L.; Adámková, A.; Babosova, R. Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive Substances. Agronomy 2020, 10, 868.

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