Next Article in Journal
A Beetle in a Haystack: Are There Alternate Hosts of the Coffee Berry Borer (Hypothenemus hampei) in Puerto Rico?
Next Article in Special Issue
Local Solutions for Sustainable Food Systems: The Contribution of Orphan Crops and Wild Edible Species
Previous Article in Journal
QTL Mapping for Drought-Responsive Agronomic Traits Associated with Physiology, Phenology, and Yield in an Andean Intra-Gene Pool Common Bean Population
Previous Article in Special Issue
Agro-Morphological Exploration of Some Unexplored Wild Vigna Legumes for Domestication
Open AccessArticle

Induced Mutagenesis Enhances Lodging Resistance and Photosynthetic Efficiency of Kodomillet (Paspalum Scrobiculatum)

1
Department of Millets, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore 641003, Coimbatore, India
2
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, Delhi, India
3
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, Coimbatore, India
4
Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore 641003, Coimbatore, India
*
Author to whom correspondence should be addressed.
Agronomy 2020, 10(2), 227; https://doi.org/10.3390/agronomy10020227
Received: 26 December 2019 / Revised: 26 January 2020 / Accepted: 27 January 2020 / Published: 4 February 2020
The present research was focused in the development of photosynthetically efficient (PhE) and non-lodging mutants by utilizing ethyl methane sulphonate (EMS) and gamma radiation in the kodomillet variety CO 3, prone to lodging. Striking variations in a number of anatomical characteristics of leaf anatomy for PhE and culm thickness for lodging resistance was recorded in M2 (second mutant) generation. The identified mutants were subjected to transcriptomic studies to understand their molecular basis. Expression profiling was undertaken for pyruvate phosphate dikinase (PPDK), Nicotinamide Adenine Dinucleotide Phosphate Hydrogen—(NADPH) and NADP-dependent malate dehydrogenase (NADP-MDH) in the mutants CO 3-100-7-12 (photosynthetically efficient) and in CO 3-200-13-4 (less efficient). For lodging trait, two mutants CO 3-100-18-22 (lodged) and CO 3-300-7-4 (non-lodged) were selected for expression profiling using genes GA2ox6 and Rht-B. The studies confirmed the expression of PPDK increased 30-fold, NADP-ME2 ~1-fold and NADP-MDH10 was also highly expressed in the mutant CO 3-100-7-12. These expression profiles suggest that kodomillet uses an NADP-malic enzyme subtype C4 photosynthetic system. The expression of Rht-B was significantly up regulated in CO 3-300-7-4. The study highlights the differential expression patterns of the same gene in different lines at different time points of stress as well as non-stress conditions. This infers that the mutation has some effect on their expression; otherwise the expression levels will be unaltered. Enhancement in grain yield could be best achieved by developing a phenotype with high PhE and culm with thick sclerenchyma cells. View Full-Text
Keywords: induced mutation; lodging resistance; photosynthetic efficiency; transcriptomics induced mutation; lodging resistance; photosynthetic efficiency; transcriptomics
Show Figures

Figure 1

MDPI and ACS Style

Jency, J.P.; Rajasekaran, R.; Singh, R.K.; Muthurajan, R.; Prabhakaran, J.; Mehanathan, M.; Prasad, M.; Ganesan, J. Induced Mutagenesis Enhances Lodging Resistance and Photosynthetic Efficiency of Kodomillet (Paspalum Scrobiculatum). Agronomy 2020, 10, 227.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop