Next Article in Journal
Do We Need New Crops for Arid Regions? A Review of Fruit Species Domestication in Israel
Previous Article in Journal
Using Temporally Resolved Floral Resource Maps to Explain Bumblebee Colony Performance in Agricultural Landscapes
 
 
Article

Comparison of the Effect of Meta-Topolin and Benzyladenine during Daphne mezereum L. Micropropagation

Section of Ornamental Plants, Institute of Horticultural Sciences, Warsaw University of Life Sciences (SGGW), 166 Nowoursynowska Str., 02-787 Warszawa, Poland
*
Author to whom correspondence should be addressed.
Agronomy 2020, 10(12), 1994; https://doi.org/10.3390/agronomy10121994
Received: 22 October 2020 / Revised: 22 November 2020 / Accepted: 15 December 2020 / Published: 18 December 2020
(This article belongs to the Section Horticultural and Floricultural Crops)
Meta-Topolin (mT) is one of the lesser-known and only recently discovered cytokinins that can be used as an alternative to the commonly used benzyladenine (BA). The experiment aimed to compare the effects of BA and mT on the regeneration from explants of Daphne mezereum, on the levels of several chemical components, the catalase activity, and to assess genetic diversity of microcuttings. The Murashige and Skoog medium (MS) and the woody plant medium (WPM) were used, enhanced with auxin and 1 mg·L−1 mT or 1 mg·L−1 BA. The results suggest that mT has a more positive effect on shoot regeneration. It also positively affected the levels of various compounds which are generally taken as indicative of micro cutting conditions. The levels of chlorophyll and carbohydrates were the highest in microcuttings from the WPM with 0.1 mg·L−1 NAA. Total soluble proteins and amino acids were the highest in plants from the MS medium with 1 mg·L−1 mT. Increased levels of hydrogen peroxide and catalase activity were observed on media with 1 mg·L−1 BA. Screening with 30 randomly amplified polymorphic DNAs (RAPD) and 20 inter-simple sequence repeat (ISSR) primers demonstrated a considerable level of genetic variation among regenerating plants. This variation is attributed to the condition of the starting material (seed propagated); there were no indications that the two cytokinins tested contributed to increased somaclonal variation. View Full-Text
Keywords: biochemical analyzes; cytokinins; in vitro; ISSR markers; RAPD markers; somaclonal variation biochemical analyzes; cytokinins; in vitro; ISSR markers; RAPD markers; somaclonal variation
Show Figures

Graphical abstract

MDPI and ACS Style

Nowakowska, K.; Pacholczak, A. Comparison of the Effect of Meta-Topolin and Benzyladenine during Daphne mezereum L. Micropropagation. Agronomy 2020, 10, 1994. https://doi.org/10.3390/agronomy10121994

AMA Style

Nowakowska K, Pacholczak A. Comparison of the Effect of Meta-Topolin and Benzyladenine during Daphne mezereum L. Micropropagation. Agronomy. 2020; 10(12):1994. https://doi.org/10.3390/agronomy10121994

Chicago/Turabian Style

Nowakowska, Karolina, and Andrzej Pacholczak. 2020. "Comparison of the Effect of Meta-Topolin and Benzyladenine during Daphne mezereum L. Micropropagation" Agronomy 10, no. 12: 1994. https://doi.org/10.3390/agronomy10121994

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop