Morpho-Physiology and Antioxidant Enzyme Activities of Transgenic Rice Plant Overexpressing ABP57 under Reproductive Stage Drought Condition
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Treatment
2.3. Morphological Analyses
2.4. Physiological Analyses
2.5. Biochemical Analyses
2.5.1. Proline Content
2.5.2. Antioxidant Enzymes Assay
2.6. Agronomical Traits and Drought Tolerance Indices Determination
2.7. Statistical Analysis
3. Results
3.1. Drought Stress Effects on Morphological Performances
3.2. Drought Stress Effects on Physiological Responses
3.3. Drought Stress Effects on Biochemical Changes
3.4. Drought Stress Effects on Agronomical Performances
3.4.1. Yield and Yield Attributes
3.4.2. Drought Tolerance Indices
3.5. Phenotypic Correlations among Morpho-Physiological, Biochemical and Agronomical Traits
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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SSI Value | Rate |
---|---|
Less than 0.50 | Highly drought-tolerant |
0.51–0.75 | Drought-tolerant |
0.76–1.00 | Moderately drought-tolerant |
More than 1.00 | Drought-susceptible |
Source of Variation | df | Mean Square | |||||
---|---|---|---|---|---|---|---|
FLA (cm2) | NOT | PH (cm) | CLDW (g plant−1) | PDW (g plant−1) | TDW (g plant−1) | ||
Replications (R) | 2 | 0.02 | 0.25 | 2.27 | 0.57 | 2.46 | 0.31 |
Water treatments (W) | 1 | 458.80 * | 126.75 | 61.20 | 102.55 | 1017.71 * | 1864.76 * |
R × W | 2 | 2.77 | 0.25 | 0.96 | 0.03 | 1.97 | 6.37 |
Genotypes (G) | 1 | 705.33 * | 630.75 * | 8.17 | 225.51 * | 4186.94 * | 6541.27 * |
G × W | 1 | 83.21 * | 0.75 | 3.31 | 12.94 | 185.57 | 337.61 |
Error | 4 | 0.27 | 1.75 | 0.74 | 0.91 | 1.68 | 3.04 |
Genotype/Treatment | FLA (cm2) | NOT | PH (cm) | CLDW (g plant−1) | PDW (g plant−1) | TDW (g plant−1) | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WW | DS | WW | DS | WW | DS | WW | DS | WW | DS | WW | DS | |
MR219 transgenic line | 41.5 a | 34.4 a | 28 a | 21 a | 95.1 b | 91.6 a | 20.5 a | 16.8 a | 94.7 a | 84.1 a | 115.2 a | 100.9 a |
Wild-type MR219 | 31.4 b | 13.8 b | 13 b | 7 b | 97.8 a | 92.2 a | 13.9 b | 6.0 b | 65.2 b | 38.9 b | 79.1 b | 43.6 b |
Mean | 36.4 | 24.1 | 21 | 14 | 96.4 | 91.9 | 17.2 | 11.4 | 79.9 | 61.5 | 97.2 | 72.2 |
CV (%) | 15.3 | 47.0 | 40.3 | 55.1 | 1.66 | 1.38 | 21.1 | 52.2 | 20.3 | 40.3 | 20.4 | 43.5 |
Source of Variation | df | Mean Square | |||
---|---|---|---|---|---|
RWC (%) | Chlorophyll Content (mg g−1 FW) | Pn (µ mol CO2 m−2s−1) | gs (mol H2O m−2s−1) | ||
Replications (R) | 2 | 9.33 | 0.19 | 0.01 | 0.01 |
Water treatments (W) | 1 | 3300.08 * | 53.78 * | 60.21 * | 0.02 * |
R × W | 2 | 4.33 | 0.14 | 0.01 | 0.01 |
Genotypes (G) | 1 | 1140.75 * | 76.93 * | 5.50 * | 0.04 * |
G × W | 1 | 784.08 * | 36.04 * | 0.02 | 0.06 * |
Error | 4 | 2.17 | 0.06 | 0.01 | 0.01 |
Source of Variation | df | Mean Square | |||
---|---|---|---|---|---|
Proline Content (mg g−1) | CAT (Units mg Protein−1) | APX (Units mg Protein) | GPX (Units mg Protein−1) | ||
Replications (R) | 2 | 0.07 | 0.01 | 0.06 | 0.03 |
Water treatments (W) | 1 | 0.97 * | 7.24 * | 4.94 * | 26.58 * |
R × W | 2 | 0.02 | 0.01 | 0.02 | 0.02 |
Genotypes (G) | 1 | 0.37 * | 2.22 * | 2.41 * | 16.10 * |
G × W | 1 | 0.24 * | 2.22 * | 1.79 * | 12.20 * |
Error | 4 | 0.04 | 0.03 | 0.06 | 0.03 |
Source of Variation | df | Mean Square | ||
---|---|---|---|---|
Grain Yield (g plant−1) | 1000-Grain Weight (g) | Spikelets/Panicle | ||
Replications (R) | 2 | 3.82 | 0.01 | 3.00 |
Water treatments (W) | 1 | 2679.94 * | 45.86 * | 602.08 * |
R × W | 2 | 0.82 | 0.01 | 2.33 |
Genotypes (G) | 1 | 4918.73 * | 83.32 * | 1850.08 * |
G × W | 1 | 1625.18 * | 19.92 * | 352.08 * |
Error | 4 | 2.54 | 0.02 | 1.33 |
Genotype/Treatment | Grain Yield (g plant−1) | 1000-Grain Weight (g) | Spikelets/Panicle | |||
---|---|---|---|---|---|---|
WW | DS | WW | DS | WW | DS | |
MR219 transgenic line | 91.77a | 85.15a | 29.19a | 27.86a | 81a | 78a |
Wild-type MR219 | 74.55b | 21.39b | 26.50b | 20.01b | 67b | 42b |
Mean | 83.16 | 53.27 | 27.85 | 23.94 | 74 | 60 |
CV (%) | 11.55 | 65.58 | 5.31 | 17.96 | 10.41 | 32.56 |
Treatment | Transgenic MR219 | Wild-Type MR219 |
---|---|---|
Well-watered plant | ||
Mean yield (g) | 91.77a | 74.55b |
RY (g) | 1.00 | 0.81 |
Mean RY (g) | 0.91 | |
Drought stress plant | ||
Mean yield (g) | 88.15a | 21.39b |
RY (g) | 1.00 | 0.25 |
Mean RY (g) | 0.63 |
Genotype | REI | MPI | MRP | TOL | STI | SSI | DTE (%) |
---|---|---|---|---|---|---|---|
Transgenic MR219 | 1.76 (1) | 88.43 (1) | 2.70 (1) | 6.61 (1) | 0.03 (1) | 0.20 (1) | 92.81 (1) |
Wild-type MR219 | 0.36 (2) | 47.97 (2) | 1.30 (2) | 53.16 (2) | 0.01 (2) | 1.98 (2) | 28.69 (2) |
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Kamarudin, Z.S.; Shamsudin, N.A.A.; Othman, M.H.C.; Shakri, T.; Tan, L.-W.; Sukiran, N.L.; Isa, N.M.; Rahman, Z.A.; Zainal, Z. Morpho-Physiology and Antioxidant Enzyme Activities of Transgenic Rice Plant Overexpressing ABP57 under Reproductive Stage Drought Condition. Agronomy 2020, 10, 1530. https://doi.org/10.3390/agronomy10101530
Kamarudin ZS, Shamsudin NAA, Othman MHC, Shakri T, Tan L-W, Sukiran NL, Isa NM, Rahman ZA, Zainal Z. Morpho-Physiology and Antioxidant Enzyme Activities of Transgenic Rice Plant Overexpressing ABP57 under Reproductive Stage Drought Condition. Agronomy. 2020; 10(10):1530. https://doi.org/10.3390/agronomy10101530
Chicago/Turabian StyleKamarudin, Zarifth Shafika, Noraziyah Abd Aziz Shamsudin, Muhamad Hafiz Che Othman, Tasneem Shakri, Lay-Wen Tan, Noor Liyana Sukiran, Nurulhikma Md Isa, Zuraida Ab Rahman, and Zamri Zainal. 2020. "Morpho-Physiology and Antioxidant Enzyme Activities of Transgenic Rice Plant Overexpressing ABP57 under Reproductive Stage Drought Condition" Agronomy 10, no. 10: 1530. https://doi.org/10.3390/agronomy10101530