Next Article in Journal
A Strategy to Enhance the Electrode Performance of Novel Three-Dimensional PEDOT/RVC Composites by Electrochemical Deposition Method
Next Article in Special Issue
Highly Branched poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) for High Performance Gene Transfection
Previous Article in Journal / Special Issue
Systematic Study of a Library of PDMAEMA-Based, Superparamagnetic Nano-Stars for the Transfection of CHO-K1 Cells
Open AccessArticle

CAGW Peptide Modified Biodegradable Cationic Copolymer for Effective Gene Delivery

1
School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China
2
School of Chemistry and Chemical Engineering, Qinghai University for Nationalities, Xining 810007, Qinghai, China
3
Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin 300072, China
4
Joint Laboratory for Biomaterials and Regenerative Medicine, Tianjin University-Helmholtz-Zentrum Geesthacht, Yaguan Road 135, Tianjin 300350, China
5
Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Yaguan Road 135, Tianjin 300350, China
6
Department of Physiology and Pathophysiology, Logistics University of Chinese People′s Armed Police Force, Tianjin 300162, China
7
Institute of Biomaterials and Engineering, Wenzhou Medical University, Wenzhou 325011, Zhejiang, China
8
Wenzhou Institute of Biomaterials and Engineering, Ningbo Institute of Industrial Technology, Chinese Academy of Sciences, Wenzhou 325011, Zhejiang, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Ravin Narain
Polymers 2017, 9(5), 158; https://doi.org/10.3390/polym9050158
Received: 8 March 2017 / Revised: 11 April 2017 / Accepted: 24 April 2017 / Published: 28 April 2017
(This article belongs to the Special Issue Polymers and Nanogels for Gene Therapy)
In recent years, gene therapy has become a promising technology to enhance endothelialization of artificial vascular grafts. The ideal gene therapy requires a gene carrier with low cytotoxicity and high transfection efficiency. In this paper, we prepared a biodegradable cationic copolymer poly(d,l-lactide-co-glycolide)-graft-PEI (PLGA-g-PEI), grafted Cys-Ala-Gly-Trp (CAGW) peptide onto this copolymer via the thiol-ene Click-reaction, and then prepared micelles by a self-assembly method. pEGFP-ZNF580 plasmids (pDNA) were condensed by these micelles via electrostatic interaction to form gene complexes. The CAGW peptide enables these gene complexes with special recognition for endothelial cells, which could enhance their transfection. As a gene carrier system, the PLGA-g-PEI-g-CAGW/pDNA gene complexes were evaluated and the results showed that they had suitable diameter and zeta potential for cellular uptake, and exhibited low cytotoxicity and high transfection efficiency for EA.hy926 cells. View Full-Text
Keywords: PLGA-g-PEI; polyethylenimine; endothelial cells; CAGW peptide; gene carrier; transfection PLGA-g-PEI; polyethylenimine; endothelial cells; CAGW peptide; gene carrier; transfection
Show Figures

Graphical abstract

MDPI and ACS Style

Duo, X.; Wang, J.; Li, Q.; Neve, A.L.; Akpanyung, M.; Nejjari, A.; Ali, Z.S.S.; Feng, Y.; Zhang, W.; Shi, C. CAGW Peptide Modified Biodegradable Cationic Copolymer for Effective Gene Delivery. Polymers 2017, 9, 158. https://doi.org/10.3390/polym9050158

AMA Style

Duo X, Wang J, Li Q, Neve AL, Akpanyung M, Nejjari A, Ali ZSS, Feng Y, Zhang W, Shi C. CAGW Peptide Modified Biodegradable Cationic Copolymer for Effective Gene Delivery. Polymers. 2017; 9(5):158. https://doi.org/10.3390/polym9050158

Chicago/Turabian Style

Duo, Xinghong; Wang, Jun; Li, Qian; Neve, Agnaldo L.; Akpanyung, Mary; Nejjari, Abdelilah; Ali, Zaidi S.S.; Feng, Yakai; Zhang, Wencheng; Shi, Changcan. 2017. "CAGW Peptide Modified Biodegradable Cationic Copolymer for Effective Gene Delivery" Polymers 9, no. 5: 158. https://doi.org/10.3390/polym9050158

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop