Next Article in Journal
Effect of Geometrical Asymmetry on the Phase Behavior of Rod-Coil Diblock Copolymers
Previous Article in Journal
Electro-Active Polymers (EAPs): A Promising Route to Design Bio-Organic/Bioinspired Platforms with on Demand Functionalities
Previous Article in Special Issue
Highly Active Copolymerization of Ethylene and N-Acetyl-O-(ω-Alkenyl)-l-Tyrosine Ethyl Esters Catalyzed by Titanium Complex

Polymers 2016, 8(5), 187; https://doi.org/10.3390/polym8050187

Article
Synthesis and Characterization of Polystyrene-Supported Piperazine-Substituted Triazoles by CuAAC and First Evaluation for Metal Ion Extraction
1
Unité de Technologies Chimiques et Biologiques pour la Santé, CNRS UMR8258, INSERM U1022, Ecole Nationale Supérieure de Chimie de Paris, PSL Research University, 11 rue Pierre & Marie Curie, Paris 75005, France
2
Faculté des Sciences de Bizerte, Université de Carthage, Jarzouna, Bizerte 7021, Tunisia
3
Institut Supérieur de Biotechnologie, Avenue Habib Bourguiba, Beja 9000, Tunisia
*
Correspondence: Tel.: +33-144-276-748 (C.G.); Fax: +33-144-276-496 (C.G.)
Received: 4 January 2016 / Accepted: 23 March 2016 / Published: 10 May 2016

Abstract

:
The goal of this work was to synthesize substituted polystyrene for metal extraction and/or depollution by introduction of substituted piperazines as chelatants starting from Merrifield polymer. After transformation of Merrifield’s resin in azidomethyl polystyrene, click-chemistry using copper (I)-catalyzed Huisgen’s reaction (CuAAC) was performed to prepare different polymers grafted with 1,4-triazoles bearing the piperazines, containing an alkyne as the other counterpart in the CuAAC. The polymers were then first tested for their efficiency to remove various metal ions from neutral aqueous solutions (Fe3+, Ni2+, Cu2+, Zn2+ and Pb2+). The polymers were found to extract Ni2+ and Zn2+ with low efficiencies ≤40%. For Fe3+ and Cu2+, the average extraction was around 80%, and for Pb2+ around 50%. The global selectivity for these polymers was found to be in the order of Fe3+ ≥ Cu2+ > Pb2+ >> Ni2+ > Zn2+.
Keywords:
Tailor-made polymers; Merrifield resin; click chemistry; piperazine triazoles; metal ion removal

1. Introduction

Water pollution by inorganic and organic contaminants is a major concern for water treatment and water stocks management on our planet. These contaminations often occur as a result of human agricultural and industrial activities, but can also be from natural origins, and can be found in all water reservoirs. Metallic ion pollution can have adverse effects on ecosystems and humans, mainly through the drinkable water quality. This kind of pollution is a major concern and solutions to prevent, monitor and treat it are still needed. Metallic cations can be removed from water using clays, natural and synthetic minerals (e.g., zeolites), ceramics and organic polymers bearing the required functions.
For a long time, what we call ion exchange resins, polymers bearing groups chelating a non-toxic metal cation such as sodium or others that can be exchanged with the cation for removal, have been used [1,2,3]. Several polymers can be made, and substitutions introduced, in order to create hydrophilic [4,5,6,7] and hydrophobic polymers [8,9], in order to use them in different applications [10,11,12,13,14]. Ideally, a polymer that can be easily functionalized for a specific application is of great interest. Such polymers can be engineered in a simple way to introduce chelating groups that can complex the metallic ions and find uses in trace detection, depollution or even supported catalysis [15,16,17,18,19,20].
Since our laboratory has been working on metal chelation for supported catalysts, we became interested in the preparation of engineered polymers [21,22,23]. We decided to introduce substituents on the polymer by using the “click-chemistry” concept and the best example of this approach, namely the copper (I)-catalyzed Huisgen's cycloaddition (“copper (I)-catalyzed azide/alkyne cycloaddition” or CuAAC) [24,25,26,27,28,29]. The use of CuAAC gives the opportunity to introduce different chelating groups on an azided polymer by selecting substituted alkynes [30,31,32,33,34,35].
As depicted in Figure 1, the introduced appendages can chelate the metallic cation (“pendant design”, a), but chelation can also occurs from the triazole linkage (“triazole design”, b), or both of these methods (“integrated design”, a,b). The chelation can also be made in mono- or multi-ligand mode, with other chelatants on the polymeric structure.
Our previous work was based on amide appendages [23], and we present in this paper the complete synthesis and characterizations of polymer-supported triazole-piperazines using Merrifield polymer (chloromethylated polystyrene) as the starting scaffold, chloride substitution to give azidomethyl polystyrene, followed by CuAAC with piperazine-substituted alkynes as carbamates of propargyl alcohol (Figure 1). The polymers were then first tested for their ability to extract some metallic cations (Fe3+, Ni2+, Cu2+, Zn2+ and Pb2+) from neutral aqueous solutions by atomic absorption spectrometry. The polymers synthesized here were also used in parallel to elaborate a high-throughput evaluation of chelation by conductometry, which has been published elsewhere [36].

2. Experimental

2.1. Materials

Merrifield polymer (chloromethylated poly-styrene-co-divinylbenzene (1%), 100–200 mesh, 2.1 mmol Cl·g−1) was purchased from Fluka / Sigma-Aldrich (St-Quentin Fallavier, France), washed alternatively three times with methanol and methylene chloride (10 mL·g−1) and dried before use. N-methylpiperazine (1a, ≥99%), sodium carbonate (≥98%), FeCl3·6H2O (97%), NiCl2·6H2O (98%), ZnSO4·7H2O (98%) and CuCl2·2H2O (97%) were issued from the same provider and used as such. Methylene chloride (99.5%), propargyl chloroformate (96%), N-ethyl (1b, 98%), 2-methoxyphenyl (1c, 98%), 2-pyrimidyl (1d, 98%), 2-pyridyl (1e, 98%), benzoyloxycarbonyl (1f, 98%) and 2-furoyl (1g, 97%) piperazines, and copper (I) iodide 99%) were provided by Sigma-Aldrich (St-Quentin Fallavier , France) and used as such. Sodium azide (99%) was supplied by Alfa-Aesar (Schiltigheim, France). Acetonitrile (99.5%), Pb(NO3)2 (99.5%) were ordered from SDS-Carlo-Erba and used as received. Methanol (97.8%), and triethylamine (99%) were bought from Prolabo/VWR (Fontenay sous Bois, France). Tetrahydrofuran (99%) was from the same source, but distilled from benzophenone sodium ketyl under nitrogen prior to use. Pyridine (99%) was ordered from Riedel-de Haën/Sigma-Aldrich (St-Quentin Fallavier, France) and used directly as furnished.

2.2. Organic Syntheses

2.2.1. Syntheses of Azidomethyl Polystyrene (A) and Piperazine Propargyl Carbamates (2a2g)

Organic syntheses (Scheme 1, Scheme 2 and Scheme 3, Section 3.1 and Section 3.2) were conducted under nitrogen, otherwise stated, using standard methods and procedures. Characterizations were made as described below. Melting points (mp) were measured on a Kofler apparatus after calibration around the observed fusion of the product. Infrared analysis using the attenuated total reflectance technique (ATR/FTIR) was made on a Nicolet FTIR 200 spectrophotometer (Thermo Scientific, Villebon sur Yvette, France) between 4000 and 400 cm−1. Only the main and relevant absorption bands are indicated as vibrations (ν) and angular deformations (δ). Nuclear magnetic resonance spectroscopy (NMR) was recorded on a Bruker Avance 300 WB spectrometer (Bruker, Champs sur Marne, France) at 300 MHz for the proton spectra (1H) and 75.5 MHz for the carbon spectra (13C). Chemical shifts (δ) are indicated after calibration on the residual undeuterated solvent peak in parts per million (ppm) and as follow: s (singlet), d (doublet), t (triplet), dd (doublet of doublet), td (triplet of doublet ), m (multiplet). Coupling constants (J) are given in Hz. High performance liquid chromatography coupled with mass spectrometry (LC-MS) were performed on a Shimadzu LC-10AD VP chromatograph using an Alltima HP C8 3 µ reverse phase column (Alltech, Carquefou, France, length = 53 mm, internal diameter = 7 mm), using a acetonitrile/0.1% formic acid in water gradient at 1 mL·min−1 using the following sequence: 0–1 min, 30% CH3CN; 1–5 min 30% to 100% CH3CN; 5–12 min, 100% CH3CN; 12–15 min, 100% to 30% CH3CN and 15–20 min 30% CH3CN. Detection of the species was done using both PDA SPD-M10A VP (diode array, D2 lamp, 190–400 nm) and ELSD-LT (low temperature evaporative light scattering) detectors. Mass spectrometry was performed on a prep LCMS-2010A (liquid chromatograph mass spectrometer) at the end of the HPLC process between m/z = 100 to 1700 in positive mode by ESI with quadrupole at 5 V and detector at 1.5 kV. Results are given in ELSD purity, retention time (Rt), and m/z of the ion.

Synthesis of Azidomethyl Polystyrene (A) (Scheme 1, Section 3.1)

The polymer A was prepared according to literature [37]. Merrifield polymer (2.10 mmol Cl·g−1, 5.00 g, 10.5 mmol Cl) was suspended in 50 mL DMSO and 5.00 g NaN3 (76.9 mmol, 7.32 eq.) were added. The reaction mixture was slowly magnetically stirred at 70 °C for 48 h. The cooled mixture was poured in water (50 mL), filtered on sintered glass and washed eight times with water (8 × 25 mL) and finally dried in an oven at 50 °C overnight. Elemental analysis of the isolated polymer gave 7.63% N and 0.10% Cl contents. Calculated substitution efficiency was 99% and the polymer has a final loading of 1.82 mmol N3·g−1.
ATR/FTIR: ν = 3036, 2934, 2864, 2103 (νN3), 1605, 1507, 1451, 1256, 816, 760, 701, 547 cm−1.

Synthesis of Propargyl Carbamate Derivatives of Piperazine 2ag (Scheme 2, Section 3.1)

To a solution of the required N-substituted piperazine 1a to 1g (12.0 mmol) in acetonitrile (45 mL) was added Na2CO3 (1.27 g, 12.0 mmol, 1 eq.). Propargyl chloroformate (1.42 g, 1.17 mL, 12.0 mmol, 1 eq.) was then added dropwise. The reaction mixture was stirred for 48 h at room temperature and then filtered and evaporated under vacuum. The resulting carbamates 2ag were sufficiently pure to be used without further purification.
Prop-2-yn-1-yl 4-methylpiperazine-1-carboxylate (2a): C9H14N2O2. Mw = 182.21 g·mol−1. Yield = 92%. Oil. ATR/FTIR: ν = 3267, 2956, 2810, 1714, 1445, 1388, 1363, 1299, 1266, 1244, 1156, 1111, 1079, 769, 685, 599 cm−1. 1H NMR (300 MHz, CDCl3, δ): 2.28 (s, 3H), 2.36 (s, 4H), 2.45 (t, J = 2.41 Hz, 1H), 3.50 (t, J = 5.09 Hz, 4H), 4.68 (d, J = 2.44 Hz, 2H) ppm. 13C NMR (75.5 MHz, CDCl3, δ): 43.73, 46.09, 52.93, 54.58, 74.56, 78.44, 154.3 ppm. LC-MS: ELSD pur. 100%; Rt = 1.582 min; m/z: 183 ([M+H]+).
Prop-2-yn-1-yl 4-ethylpiperazine-1-carboxylate (2b): C10H16N2O2. Mw = 196.24 g·mol−1. Yield = 88%. Oil. ATR/FTIR: ν = 3264, 2987, 2828, 2782, 1715, 1445, 1388, 1360, 1289, 1249, 1137, 1086, 1034, 769, 684, 597 cm−1. 1H NMR (300 MHz, CDCl3, δ): 1.06 (t, J = 7.21 Hz, 3H), 2.4 (m, 7H), 3.50 (t, J = 5.11 Hz, 4H), 4.68 (d, J = 4.28 Hz, 2H) ppm. 13C NMR (75.5 MHz, CDCl3, δ): 11.91, 43.88, 52.34, 52.44, 52.94, 74.48, 78.53, 154.3 ppm. LC-MS: ELSD pur. 100%; Rt = 1.637 min; m/z: 197 ([M+H]+.
Prop-2-yn-1-yl 4-(2-methoxyphenyl)piperazine-1-carboxylate (2c): C15H18N2O3. Mw = 274.31 g·mol−1. Yield = 99%. mp = 105 °C. ATR/FTIR: ν = 3250, 3080, 3006, 2893, 2834, 1686, 1596, 1506, 1444, 1363, 1281, 1248, 1155, 1093, 1028, 988, 747, 587 cm−1. 1H NMR (300 MHz, CDCl3, δ): 2.48 (t, J = 1.83 Hz, 1H), 3.01 (s, 4H), 3.67 (t, J = 4.84 Hz, 4H), 3.86 (s, 3H), 4.72 (d, J = 1.83 Hz, 2H), 6.99 (m, 4H) ppm. 13C NMR (75.5 MHz, CDCl3, δ): 44.19, 50.53, 52.97, 55.44, 74.80, 78.60, 111.5, 118.5, 121.1, 123.5, 140.9, 152.3, 154.3 ppm. LC-MS: ELSD pur. 100%; Rt = 4.845 min; m/z: 275 ([M+H]+).
Prop-2-yn-1-yl 4-(pyrimidin-2-yl)piperazine-1-carboxylate (2d): C12H14N4O2. Mw = 246.26 g·mol−1. Yield = 99%. mp = 120 °C. ATR/FTIR: ν = 3270, 3043, 2942, 2884, 1726, 1596, 1514, 1448, 1551, 1284, 1241, 1131, 1095, 1022, 986 , 960, 795, 686, 498 cm−1. 1H NMR (300 MHz, CDCl3, δ): 2.51 (t, J = 2.45 Hz, 1H), 3.60 (t, J = 5.25 Hz, 4H), 3.86 (t, J = 5.06 Hz, 4H), 4.70 (d, J = 2.46 Hz, 2H), 6.55 (t, J = 4.76 Hz, 1H), 8.34 (d, J = 4.75 Hz, 2H) ppm. 13C NMR (75.5 MHz, CDCl3, δ): 43.44, 43.74, 53.15, 74.70, 78.42, 110.4, 154.5, 157.8, 161.5 ppm. LC-MS: ELSD pur. 100%; Rt = 4.251 min; m/z: 247 ([M+H]+).
Prop-2-yn-1-yl 4-(pyridin-2-yl)piperazine-1-carboxylate (2e): C13H15N3O2. Mw = 245.27 g·mol−1. Yield = 99%. mp = 92 °C. ATR/FTIR: ν = 3261, 2956, 2873, 1703, 1601, 1487, 1441, 1571, 1289, 1242, 1170, 1123, 1093, 984, 777, 742, 696, 584 cm−1. 1H NMR (300 MHz, CDCl3, δ): 2.52 (t, J = 2.44 Hz, 1H), 3.60 (m, 8H), 4.77 (d, J = 2.45 Hz, 2H), 6.69 (t, J = 6.06 Hz, 1H), 7.53 (td, J = 7.86 Hz, J = 2.09 Hz, 2H), 8.23 (dd, J = 4.76 Hz, J = 1.02 Hz, 1H) ppm. 13C NMR (75.5 MHz, CDCl3, δ): 43.60, 45.02, 53.09, 74.70, 78.44, 107.3, 113.8, 137.7, 148.2, 154.4, 159.1 ppm. LC-MS: ELSD pur. 100%; Rt = 1.808 min; m/z: 247 ([M+2H]+).
Prop-2-yn-1-yl 4-(benzyloxycarbonyl)piperazine-1-carboxylate (2f): C16H18N2O4. Mw = 302.32 g·mol−1. Yield = 91%. mp = 76 °C. ATR/FTIR: ν = 3248, 2953, 2914, 2882, 1697, 1507, 1438, 1360, 1291, 1238, 1110, 990, 952, 919, 868, 758, 591, 557 cm−1. 1H NMR (300 MHz, CDCl3, δ): 2.51 (t, J = 2.45 Hz, 1H), 3.49 (m, 8H), 4.71 (d, J = 2.46 Hz, 2H), 5.15 (s, 2H), 7.34–7.38 (m, 5H) ppm. 13C NMR (75.5 MHz, CDCl3, δ): 43.61, 53.19, 67.43, 74.89, 78.28, 128.0, 128.2, 128.6, 136.4, 154.3, 155.1 ppm. LC-MS: ELSD pur. 96%; Rt = 5.213 min; m/z: 343 ([M+MeCN]+).
Prop-2-yn-1-yl 4-(furan-2-carbonyl)piperazine-1-carboxylate (2g): C13H14N2O4. Mw = 262.26 g·mol−1. Yield = 99%. mp = 102 °C. ATR/FTIR: ν = 3238, 3129, 2947, 2889, 1718, 1631, 1589, 1497, 1432, 1355, 1286, 1255, 1191, 1123 ,1087, 1022, 769, 710, 598 cm−1. 1H NMR (300 MHz, CDCl3, δ): 2.51 (t, J = 2.45 Hz), 3.6 (m, 4H), 3.82 (t, J = 0.33 Hz, 4H), 4.74 (d, J = 2.45 Hz, 2H), 6.51(dd, J = 3.49 Hz, J = 1.78 Hz, 1H), 7.06 (dd, J = 3.49 Hz, J = 0.85Hz, 1H), 7.51 (dd, J = 1.78 Hz, J = 0.87 Hz, 1H) ppm. 13C NMR (75.5 MHz, CDCl3, δ): 54.64, 63.90, 86.18, 89.19, 122.2, 127.5, 154.9, 158.3, 164.9, 169.7 ppm. LC-MS: ELSD pur. 100%; Rt = 3.993 min; m/z: 263 ([M+H]+).

2.2.2. Synthesis of Polymer-Supported Triazolic Piperazines 3ag (Scheme 3, Section 3.2)

Coupling reactions onto the polymer using CuAAC were conducted accordingly to the general procedure indicated below in round bottom flasks equipped with a reflux condenser. To a suspension of 3.00 g of azidomethyl polystyrene A (1.82 mmol N3·g−1, 5.46 mmol N3) in THF (60 mL) was added 6.30 mmol (1.15 eq.) of the alkyne (2ag), 9.00 ml of triethylamine (6.75 g, 66.7 mmol, 12.2 eq.) and 2.40 mg of copper (I) iodide (12.6 µmol, 4 mol %). The suspension was slowly stirred at room temperature 72 h. After this time, the complete disappearance of the IR band of the azide of the polymer A (2103 cm−1) was observed. The resulting polymer was filtered on sintered glass and washed sequentially with CH2Cl2, pyridine, and MeOH (60 mL each), the sequential washings were repeated twice. The resulting polymers 3ag were finally dried overnight in an oven at 50 °C.
1'-[(1',2',3'-triazol-4'-yl)methyl 4-methyl-piperazine-1-carboxylate]methyl polystyrene (3a): Yellow powder. 1.50 mmol pipera-zine·g−1. ATR/FTIR: ν = 3039, 2942, 2872, 2809, 1704, 1621, 1511, 1441, 1380, 1298, 1236, 1153, 1112, 1055, 1008, 820, 767, 702, 551 cm−1.
1'-[(1',2',3'-triazol-4'-yl)methyl 4-ethylpi-perazine-1-carboxylate]methyl polystyrene (3b): Orange powder. 1.47 mmol piperazi-ne·g−1. ATR/FTIR: ν = 3038, 2938, 2872, 2827, 1705, 1613, 1507, 1442, 1382, 1289, 1249, 1134, 1060, 1028, 967, 820, 765, 702, 547 cm−1.
1'-[(1',2',3'-triazol-4'-yl)methyl-4-(2-methoxyphenyl)piperazine-1-carboxylate]methyl polystyrene (3c): Brown powder. 1.32 mmol piperazine·g−1. ATR/FTIR: ν = 3037, 2934, 2864, 1706, 1603, 1506, 1446, 1383, 1287, 1245, 1126, 1087, 1033, 946, 753, 702, 547 cm−1.
1'-[(1',2',3'-triazol-4'-yl)methyl-4-(pyrimidin-2-yl)piperazine-1-carboxylate]methyl polystyrene (3d): Yellow powder. 1.37 mmol piperazine·g−1. ATR/FTIR: ν = 3039, 2935, 2870, 1706, 1591, 1502, 1438, 1556, 1298, 1242, 1132, 1092, 1054, 981, 800, 761, 703, 555 cm−1.
1'-[(1',2',3'-triazol-4'-yl)methyl-4-(pyridin-2-yl)piperazine-1-carboxylate]methyl poly-styrene (3e): Brown powder. 1.37 mmol piperazine·g−1. ATR/FTIR: ν = 3037, 2938, 2864, 1705, 1601, 1485, 1437, 1382, 1285, 1235, 1171, 1128, 1053, 981, 837, 771, 703, 534 cm−1.
1'-[(1',2',3'-triazol-4'-yl)methyl-4-(benzyl-oxycarbonyl)piperazine-1-carboxylate]methyl polystyrene (3f): Pale pink powder. 1.27 mmol piperazine·g−1. ATR/FTIR: ν = 3039, 2934, 2869, 1706, 1613, 1507, 1429, 1365, 1293, 1231, 1101, 1056, 1016, 980, 830, 763, 703, 546 cm−1.
1'-[(1',2',3'-triazol-4'-yl)methyl-4-(furan-2-carbonyl)piperazine-1-carboxylate]methyl polystyrene (3g): Beige powder. 1.34 mmol piperazine·g−1. ATR/FTIR: ν = 3038, 2937, 2859, 1706, 1634, 1580, 1494, 1430, 1373, 1289, 1234, 1186, 1122, 1055, 1017, 843, 759, 703, 547 cm−1.

2.3. Characterizations by ATR/FTIR of Polymer-Supported Triazolic Piperazines

Infrared analyses using the attenuated total reflectance technique (ATR/FTIR) were recorded as previously described for organic compounds (vide supra) Results appear in Figure 2, Section 3.1.

2.4. Metal Ion Extraction Method and Quantification

Neutral aqueous solutions of metal salts were prepared with 50 mg of FeCl3·6H2O (0.185 mmol), CuCl2·2H2O (0.293 mmol), NiCl2·6H2O (0.210 mmol), ZnSO4·7H2O (0.174 mmol), and Pb(NO3)2 (0.151 mmol) in 1 L of distilled water.
Aliquots of 250 mg of each polymer (3a: 0.342 mmol, 3b: 0.335 mmol, 3c: 0.302 mmol, 3d: 0.315 mmol, 3e: 0.315 mmol, 3f: 0.292 mmol, 3g: 0.307 mmol,) were incubated in triplicate with 20 mL (50 mg·L−1, 1 mg of salt) of each metal ion solution (3.70 µmol Fe3+, 5.87 µmol Cu2+, 4.21 µmol Ni2+, 3.48 µmol Zn2+, and 3.02 µmol Pb2+) at 25 °C for 24 h. The suspension was then filtered on distilled water prewashed filter paper. Evaluation of the chelated metal was done by concentration measurement by atomic absorption spectrometry on a Perkin Elmer type PinAAcle 900 T, after calibration curves recording for all metals. The results, average of three experiments, were expressed as percentages of extraction of the metal (Figure 3, Section 3.3).

3. Results and Discussion

3.1. Synthesis and Characterization of Azidomethyl Polystyrene (A) and Propargyl Carbamate Derivatives of Piperazine 2ag

Following the method of Mioskowski et al. [37], Merrifield’s polymer was treated with an excess of sodium azide (7.32 eq.) in DMSO at 70 °C for 48 h (Scheme 1). The azidomethyl polystyrene (A) prepared using this method was submitted to elemental analysis, which gave results of 7.63% for N and 0.10% for Cl. The substitution yield was of 99%, and the polymer has a final loading of 1.82 mmol N3·g−1. The presence of the azido group was confirmed by IR spectroscopy on which its strong characteristic absorption was seen at 2103 cm−1 νN3).
The required coupling partners for A were prepared using commercially available N-substituted piperazines (1ag) and reacting them with propargyl chloroformate in the presence of sodium carbonate in acetonitrile at room temperature for 48 h (Scheme 2). The corresponding propargyl carbamates 2ag were obtained in good yields (88%–99%), without the need of a purification step. Structures of the products 1ad were confirmed by their characteristic signals in NMR (ca. δ = 2.5 (triplet, ≡C–H) and 4.7 (doublet, OCH2C≡CH) ppm in 1H-NMR; 75/78 (CC) and 154 (C=O) ppm in 13C-NMR), IR spectroscopies (ca. 3250 (ν≡C–H) and 1700 (νC=O) cm−1), as well as by LC-MS.

3.2. Synthesis and ATR/FTIR Analysis of Polymer-Supported Triazolic Piperazines 3a–g

Piperazine propargyl carbamates 2ag (1.15 eq.) and azidomethyl polystyrene (A) were coupled together following a CuAAC procedure (4 mol % CuI in the presence of Et3N) in order to obtain the corresponding polymer-supported 1,2,3-triazoles-piperazines 3ag (Scheme 3). The reactions were conducted at room temperature for 72 h, after which time the azide vibration (νN3 = 2103 cm−1) completely disappeared from the resulting polymers. FTIR spectra of the synthesized polymers 3ag did not show any residual vibration band from the azido group (νN3, ca. 2100 cm−1), as previously mentioned, in either of the alkynes (ν≡C–H, ca. 3250 cm−1), as shown in Figure 2. Characteristic vibration for the carbamate bonds (νC=O, ca. 1700 cm−1), and other vibrations due to the triazole, the piperazine and the substituents, can be observed when compared to the spectrum of polymer A. The reactions were considered as quantitative based on ATR/FTIR analyses and gave polymers with triazole-piperazine loadings between 1.17 and 1.37 mmol piperazine·g−1 (Table 1).

3.3. Metal Ion Extractions by the Polymer-Supported Triazolic Piperazines 3a–g

After 24 h incubation on a 250 mg scale (~0.3 mmol) of the polymers (see Table 1) in 20 mL of 50 mg·L−1 solutions of the salts (1 mg of salt, see Section 2.4 for details), the percentages of extraction for each metal were calculated by AAS, each experiment having been carried out in triplicate. The results for each polymer as a function of the metallic cations are presented in Figure 3.
Extraction results showed than Fe3+ is well extracted by all the synthesized polymers. The maximum levels of extraction for this metal were obtained with polymers 3a (85.40% ± 3.01%) and 3b (83.67% ± 3.87%).
Other extraction efficiencies were correct, with the minimum encountered with 3d (73.14% ± 1.53%), being in the range of 75%–81%, in the following decreasing order: 3e (81.16% ± 8.02%), 3c (81.29% ± 6.26%), 3g (75.33% ± 0.19%), and 3f (75.20% ± 2.38%). The average iron (III) extraction for this series of polymers was around 79%
The second well-extracted metal ion was copper (II), with some higher values than those for Fe3+. Once again, the best extractions were found with polymers 3a (87.27% ± 2.52%) and 3b (94.00% ± 1.22%), together with 3c (90.79% ± 5.27%). The efficiencies for Cu2+ removal were then separated in two groups, with 3d (81.64% ± 0.05%) and 3e (78.94% ± 1.61%) near the first ones, then 3g (57.37% ± 3.63%) and 3f (51.80% ± 3.32%) being at a medium level. Average extraction for the polymeric series for Cu2+ was near 77%.
For Pb2+ capture from the aqueous solutions by these piperazine-triazolic polymers, it did not follow exactly the same order as for the two previous metals, with an average extraction in the range of 50%. There was an obvious difference between 3a (48.53% ± 0.71%) efficiency being in the average range, and the strong loss for 3b (18.93% ± 2.73%), being the less powerful extracting polymer. Poly(styrenes) 3d (64.27% ± 0.81%), 3c (62.68% ± 1.17%) and 3e (59.67 ± 4.26%) were more or less 10% above the average, and 3g (54.89% ± 2.44%) and 3f (46.28% ± 8.71%) around it.
For Ni2+, extraction efficiencies were in a narrow distribution around an average value of 32%. None of the studied polymers seemed to retain more of this metallic ion. The results were the following: 3a (29.37% ± 3.20%), 3b (34.68% ± 4.59%), 3c (29.28% ± 1.06%), 3d (34.98% ± 0.17%), 3e (28.91% ± 2.89%), 3f (31.87% ± 5.57%) and 3g (36.44% ± 8.25%).
Finally, the least-extracted metal was zinc (II). As for Ni2+, none of the polymers was much better than the low average of 16%. Polymers 3a (14.53% ± 4.20%), 3b (20.45% ± 4.80%), 3c (17.55% ± 4.58%), 3d (12.01% ± 5.09%), 3e (16.97% ± 0.38%), 3f (21.01% ± 6.17%) and 3g (13.34% ± 2.39%) showed a distribution again not very far from the calculated average.
When looking at all the results, as an average of all polymers, we can observe that the metals that are the best extracted are iron (III) and copper (II), at around 80%, lead (II) is extracted at around 50%, and nickel (II) and zinc (II) at 15%–30%. These results suggest that, by selecting the right polymer, a discrimination and selectivity for at least iron (III) and copper (II), and maybe lead (II), can be done over nickel (II) and zinc (II).
Since the polymeric structure differs only by the R substituent on the nitrogen (Scheme 3), a tentative analysis has been done to try to understand the influence of the substituent’s nature on the extraction efficiencies. Polymers 3a and 3b have an alkyl group on the terminal nitrogen, and are thus amines. For 3ce, and aromatic ring is the substituent, and the compounds are aniline derivatives.
Finally, for 3f and 3g, they include carbonyl linkages, and are in the carbamate and amide families, respectively. Considering only the R substituent, the availability of the nitrogen electron doublet for chelation should theoretically be in the following order: 3a,b > 3c–e > 3f,g. It should be noted, however, that polymer 3e also includes a pyridyl group, capable of metal chelation.
For iron (III), the nature of the N-terminal substituent of the piperazine does not seem to have a determinant effect on the chelation of this metal. Extraction efficiencies are within the same range of 70%–85%. Only a small decrease in efficiency can be observed in the previously cited order (3a,b > 3c–e > 3f,g), which may not be that significant.
In the case of Cu2+, a more obvious effect seems to take place, especially for carbamate (3f) and amide (3g) containing N-substituents. Polymers 3a and b are a little more efficient (ca. 90%) than 3ce (ca. 80%), but there is a more obvious decrease of around 30% while using polymers 3f and 3g.
When looking at lead (II) extraction, the effects are more puzzling. N-alkyl-substituted polymers 3a and 3b are not as efficient as was the case for the two previous metallic ions, with ~50% and ~20% extraction, respectively. Aryl-substituted polymers 3c–e are better (60%), while 3f and 3g are going down to the 3a level.
For Ni2+and Zn2+, none of the polymers extracted them well. No conclusion can be drawn other than that these polymers are not suitable for their extraction.
The electro-negativities of the substituted nitrogen of the piperazine may partially explain some of the relative extraction efficiencies, at least for iron (III) and copper (II). For lead (II), the alkyl series (3a,b) is not really matching the previsions, while the other series do. For Ni2+ and Zn2+, none of the results can give an idea about this influence since they are not well extracted, and the levels are the same for all polymers studied here. These results may indicate that the awaited “pendant design” is not the major mode of complexation encountered in this series. It may be, in part, done by an integrated mode, or it may mainly be driven by the triazole one (Figure 1). Iron (III) and copper (II) are known to be “nitrogen-philic” metals, especially for trinitrogen-/azide-type species. In a previous work, such an effect has been observed [21,22,23].
Another influence of these terminal N-substituents can be the hydrophilic/hydrophobic balance of the polymers. This may explain the access and/or stability of complexes formed between the metal the triazole nucleus. This effect has already been observed in the case of copper (II) and lead (II) in a work on PVC-supported triazolic amides [38].
We have also tried to rationalize the interactions between the polymers and the metal ions based on their electropositivities, ionic radii and water solvation. The extraction efficiencies, based on the metal cation size or electro-positivities, cannot be taken into account since only lead (II) has a superior size and lower electro-positivity when compared to the transition metals having similar radii.
When studying the water solvation of the different metals used here, maybe some explanation can be found when trying to link metal and polymer properties. In Table 2, these are presented in decreasing order of removal of metallic cations. The pH of the initial solution of the cation (pHi) and the average pH after extraction by all polymers (pHf) are reported. We can observe that the pH of the solutions were between 3.2 and 7.4, and that there is no direct link between them and the extraction order. The pH at which the extraction is performed is important since a speciation can already be performed as a function of the metal ion [39]. In these cases, a “pH50”, a pH at which 50% of the cation is extracted, can be calculated. The pH50 values can be correlated in a linear way with another value, the first hydrolysis constant in water (pK1), related to the reaction M(OH2)mn+ = M(OH2)m−1(OH)(n−1)+ + H+ [39,40]. When looking at pK1, it can be observed that when the value increases, the extraction of the cation decreases almost accordingly. pH50 is very dependent on the substrate used for cation removal, but always is in linear relation with pK1 as previously mentioned. From published studies, the order of extraction and pH50/pK1 values increases in the order of Cu ≥ Pb >> Zn > Ni. From our results, it seems that for Fe3+, Cu2+ and Pb2+, the solutions as a function of the polymers are between pH50 and pH90. For Ni2+ and Zn2+, the extractions were never excellent, maybe being in a zone between pH10 and pH40. These results thus give the following order of extraction: Fe3+ ≥ Cu2+ > Pb2+ >> Ni2+ > Zn2+.
All the results and analyses cannot definitively identify the discrete complexation behavior of the metal by the polymers at the solid/liquid interface. However, we were able to obtain results that begin to give a better understanding of the subtle equilibrium between all factors influencing the extraction efficiencies of such polymers.

4. Conclusions

In this work, we studied the chemical grafting of piperazine-chelating units onto commercial poly[styrene] (Merrifield’s resin) using the CuAAC procedure between the azided polymer and selected piperazine-N-propargyl carbamates. The synthesized polymers were characterized by FTIR. They were then tested for their efficiency to extract metallic ions from aqueous solution, including Fe3+, Ni2+, Cu2+, Zn2+ and Pb2+. All polymers were found to extract Fe3+ and Cu2+ well.
Most of the polymers were found to have a good extraction potential for Pb2+. None of the polymers were able to extract Ni2+ and Zn2+ with good efficiencies, thus showing certain selectivity when compared to the other studied metallic ions. As an added proof, the extraction efficiencies were studied on 13 metallic cations by a “high-throughput” approach using conductometry [36]. Even if the method was less precise and sensitive, similar average affinities were found for Fe3+, Ni2+ and Pb2+ (13%–17% lower).
In this series, we obtained far better extraction results than with our first series of triazolic polymers, based on poly(styrene) and poly(vinyl chloride) backbones, bearing propargyl amides and propiolic anilides [23,38].
Even if no clear interpretation can be done with the results for the interfacial chelation process, the good extraction properties encourage us to continue polymer modifications using CuAAC in order to find new triazole-linked polymeric complexing agents for depollution, trace detection and catalytic applications [41,42,43,44,45,46,47]. Further studies will be reported in due course.

Acknowledgments

This project was financed by Tunisian 05/UR/12-05 and French UMR 8151 CNRS/U1022 INSERM grants. Riadh Slimi is grateful to the Tunisian Ministry of Research and Education for fellowship.

Author Contributions

Abid Ouerghui and Christian Girard conceived and designed the experiments; Riadh Slimi, Raja Ben Othman, Noomene Sleimi and Abid Ouerghui performed the experiments; Abid Ouerghui and Christian Girard analyzed the data; Abid Ouerghui, Christian Girard and Noomene Sleimi contributed reagents/materials/analysis tools; Abid Ouerghui and Christian Girard wrote the paper.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Rifi, E.H.; Leroy, M.J.F.; Brunette, J.P.; Schloesser-Becker, C. Extraction of copper, cadmium and related metals with poly(sodium acrylate-acrylic acid) hydrogels. Solvent Extr. Ion Exch. 1994, 12, 1103–1119. [Google Scholar] [CrossRef]
  2. Hodgkin, J.H.; Eibl, R. Gold extraction with poly(diallylamine) resins. React. Polym. 1988, 9, 285–291. [Google Scholar] [CrossRef]
  3. Rivas, B.L.; Klattenhoff, D.; Perich, I.M. Kinetics of uranium sorption from acidic sulphate solutions onto crosslinked polyethyleneimine based resins. 10. Polym. Bull. 1990, 23, 219–223. [Google Scholar] [CrossRef]
  4. Anspach, W.M.; Marinsky, J.A. Complexing of nickel(II) and cobalt(II) by a polymethacrylic acid gel and its linear polyelectrolyte analog. J. Phys. Chem. 1975, 79, 433–439. [Google Scholar] [CrossRef]
  5. Nishide, H.; Oki, N.; Tsuchida, E. Complexation of poly(acrylic acid)s with uranyl ion. Eur. Polym. J. 1982, 18, 799–802. [Google Scholar] [CrossRef]
  6. Pollack, G.H. Water, energy and life: Fresh views from the water’s edge. Int. J. Des. Nat. Ecodyn. 2010, 5, 27–29. [Google Scholar] [CrossRef] [PubMed]
  7. Rifi, E.H.; Rastegar, F.; Brunette, J.P. Uptake of cesium, strontium and europium by a poly(sodium acrylate-acrylic acid) hydrogel. Talanta 1995, 42, 811–816. [Google Scholar] [CrossRef]
  8. Wang, X.; Weiss, R.A. A facile method for preparing sticky, hydrophobic polymer surfaces. Langmuir 2012, 28, 3298–3305. [Google Scholar] [CrossRef] [PubMed]
  9. Loret, J.F.; Brunette, J.P.; Leroy, M.J.F.; Candau, S.J.; Prévost, M. Liquid-lipophilic gel extraction of precious metals. Solvent Extr. Ion Exch. 1988, 6, 585–603. [Google Scholar] [CrossRef]
  10. Nghiem, L.D.; Mornane, P.; Potter, I.D.; Perera, J.M.; Cattrall, R.W.; Kolev, S.D. Extraction and transport of metal ions and small organic compounds using polymer inclusion membranes (PIMs). J. Membr. Sci. 2006, 281, 7–41. [Google Scholar] [CrossRef]
  11. Peterson, J.; Nghiem, L.D. Selective extraction of cadmium by polymer inclusion membranes containing PVC and Aliquat 336: Role base polymer and extractant. IJETM 2010, 12, 359–368. [Google Scholar] [CrossRef]
  12. Kebiche Senhadji, O.; Sahi, S.; Kahloul, N.; Tingry, S.; BenAmor, M.; Seta, P. Extraction du Cr(VI) par membrane polymère à inclusion. Sci. Technol. A 2008, 27, 43–50. [Google Scholar]
  13. Guibaud, G.; Baudu, M.; Dollet, P.; Condat, M.L.; Dagot, C. Role des polymeres extracellulaires dans l’adsorption du cadmium par les boues activées role of extracellular polymers in cadmium adsorption by activated sludges. Environ. Technol. 1999, 20, 1045–1054. [Google Scholar] [CrossRef]
  14. Upitis, A.; Peterson, J.; Lukey, C.; Nghiem, L.D. Metallic ion extraction using polymer inclusion membranes (PIMs): Optimising physical strength and extraction rate. Desalin. Water Treat. 2009, 6, 41–47. [Google Scholar] [CrossRef]
  15. Zotti, G.; Zecchin, S.; Schiavon, G.; Berlin, A.; Penso, M. Ionochromic and potentiometric properties of the novel polyconjugated polymer from anodic coupling of 5,5’-Bis(3,4-(ethylenedioxy)thien-2-yl)-2,2’-bipyridine. Chem. Mater. 1999, 11, 3342–3351. [Google Scholar] [CrossRef]
  16. Brembilla, A.; Cuny, J.; Roizard, D.; Lochon, P. Un nouveau polymère catalyseur bifonctionnel: Le polyvinyl-5 (6)benzimidazoleméthanethiol Synthèse et catalyse de l’hydrolyse de l’acétate de p-nitrophényle. Eur. Polym. J. 1983, 19, 729–735. [Google Scholar] [CrossRef]
  17. Rhazi, M.; Desbrières, J.; Tolaimate, A.; Rinaudo, M.; Vottero, P.; Alagui, A.; El Meray, M. Influence of the nature of the metal ions on the complexation with chitosan: Application to the treatment of liquid waste. Eur. Polym. J. 2002, 38, 1523–1530. [Google Scholar] [CrossRef]
  18. Kozlowski, C.A.; Walkowiak, W. Applicability of liquid membranes in chromium(VI) transport with amines as ion carriers. J. Membr. Sci. 2005, 266, 143–150. [Google Scholar] [CrossRef]
  19. Kozlowski, C.; Apostoluk, W.; Walkowiak, W.; Kita, A. Removal of Cr(VI), Zn(II) and Cd(II) ions by transport across polymer inclusion membranes with basic ion carriers. Physicochem. Probl. Miner. Process. 2002, 36, 115–122. [Google Scholar]
  20. Toy, P.H.; Janda, K.D. Soluble polymer-supported organic synthesis. Acc. Chem. Res. 2000, 33, 546–554. [Google Scholar] [CrossRef] [PubMed]
  21. Girard, C.; Önen, E.; Aufort, M.; Beauvière, S.; Samson, E.; Herscovici, J. Reusable polymer-supported catalyst for the [3+2] huisgen cycloaddition in automation protocols. Org. Lett. 2006, 8, 1689–1692. [Google Scholar] [CrossRef] [PubMed]
  22. Jlalia, I.; Beauvineau, C.; Beauvière, S.; Onen, E.; Aufort, M.; Beauvineau, A.; Khaba, E.; Herscovici, J.; Meganem, F.; Girard, C. Automated synthesis of a 96 product-sized library of triazole derivatives using a solid phase supported copper catalyst. Molecules 2010, 15, 3087–3120. [Google Scholar] [CrossRef] [PubMed]
  23. Ouerghui, A.; Elamari, H.; Ghammouri, S.; Slimi, R.; Meganem, F.; Girard, C. Polystyrene-supported triazoles for metal ions extraction: Synthesis and evaluation. Reac. Funct. Polym. 2014, 74, 37–45. [Google Scholar] [CrossRef]
  24. Li, C.; Finn, M.G. Click chemistry in materials synthesis. II. Acid-swellable crosslinked polymers made by copper-catalyzed azide–alkyne cycloaddition. J. Polym. Sci. A Polym. Chem. 2006, 44, 5513–5518. [Google Scholar] [CrossRef]
  25. Tasdelen, M.A.; Yilmaz, G.; Iskin, B.; Yagci, Y. Photoinduced free radical promoted copper(I)-catalyzed click chemistry for macromolecular syntheses. Macromolecules 2012, 45, 56–61. [Google Scholar] [CrossRef]
  26. Liu, Y.; Díaz, D.D.; Accurso, A.A.; Sharpless, K.B.; Fokin, V.V.; Finn, M.G. Click chemistry in materials synthesis. III. Metal-adhesive polymers from Cu(I)-catalyzed azide–alkyne cycloaddition. J. Polym. Sci. A Polym. Chem. 2007, 45, 5182–5189. [Google Scholar] [CrossRef]
  27. Dag, A.; Durmaz, H.; Demir, E.; Hizal, G.; Tunca, U. Heterograft copolymers via double click reactions using one-pot technique. J. Polym. Sci. A Polym. Chem. 2008, 46, 6969–6977. [Google Scholar] [CrossRef]
  28. Opsteen, J.A.; Brinkhuis, R.P.; Teeuwen, R.L.M.; Löwik, D.W.P.M.; van Hest, J.C.M. “Clickable” polymersomes. Chem. Commun. 2007, 3136–3138. [Google Scholar] [CrossRef] [PubMed]
  29. Riva, R.; Schmeits, S.; Jérôme, C.; Jérôme, R.; Lecomte, P. Combination of ring-opening polymerization and “click chemistry”: Toward functionalization and grafting of poly(ε-caprolactone). Macromolecules 2007, 40, 796–803. [Google Scholar] [CrossRef]
  30. Löber, S.; Rodriguez-Loaiza, P.; Gmeiner, P. Click linker: Efficient and high-yielding synthesis of a new family of SPOS resins by 1,3-dipolar cycloaddition. Org. Lett. 2003, 5, 1753–1755. [Google Scholar] [CrossRef] [PubMed]
  31. Iskin, B.; Yilmaz, G.; Yagci, Y. ABC type miktoarm star copolymers through combination of controlled polymerization techniques with thiol-ene and azide-alkyne click reactions. J. Polym. Sci. A Polym. Chem. 2011, 49, 2417–2422. [Google Scholar] [CrossRef]
  32. Xue, X.; Zhu, J.; Zhang, Z.; Cheng, Z.; Tu, Y.; Zhu, X. Synthesis and characterization of azobenzene-functionalized poly(styrene)-b-poly(vinyl acetate) via the combination of RAFT and “click” chemistry. Polymer 2010, 51, 3083–3090. [Google Scholar] [CrossRef]
  33. Shin, J.A.; Lim, Y.G.; Lee, K.H. Synthesis of polymers including both triazole and tetrazole by click reaction. Bull. Korean Chem. Soc. 2011, 32, 547–552. [Google Scholar] [CrossRef]
  34. Struthers, H.; Mindt, T.L.; Schibli, R. Metal chelating systems synthesized using the copper(I) catalyzed azide-alkyne cycloaddition. Dalton Trans. 2009, 39, 675–696. [Google Scholar] [CrossRef] [PubMed]
  35. Urankar, D.; Pinter, B.; Pevec, A.; de Proft, F.; Turel, I.; Košmrlj, J. Click-triazole N2 coordination to transition-metal ions is assisted by a pendant pyridine substituent. Inorg. Chem. 2010, 49, 4820–4829. [Google Scholar] [CrossRef] [PubMed]
  36. Slimi, R.; Girard, C. “High-throughput” evaluation of polymer-supported triazolic appendages for metallic cations extraction. Metals 2015, 5, 418–427. [Google Scholar] [CrossRef]
  37. Arseniyadis, S.; Wagner, A.; Mioskowski, C. A straightforward preparation of amino–polystyrene resin from Merrifield resin. Tetrahedron Lett. 2002, 43, 9717–9719. [Google Scholar] [CrossRef]
  38. Ouerghui, A.; Elamari, H.; Dardouri, M.; Ncib, S.; Meganem, F.; Girard, C. Chemical modifications of poly(vinyl chloride) to poly(vinyl azide) and “clicked” triazole bearing groups for application in metal cation extraction. React. Funct. Polym. 2016, 100, 191–197. [Google Scholar] [CrossRef]
  39. Eldridge, D.S.; Crawford, R.J.; Harding, I.H. The role of metal ion-ligand interactions during divalent metal ion adsorption. J. Colloid Interface Sci. 2015, 454, 20–26. [Google Scholar] [CrossRef] [PubMed]
  40. Gilson, R.; Durrant, M.C. Estimation of the pKa values of water ligands in transition metal complexes using density functional theory with polarized continuum model solvent corrections. Dalton Trans. 2009, 10223–10230. [Google Scholar] [CrossRef] [PubMed]
  41. Chan, T.R.; Fokin, V.V. Polymer-supported copper(I) catalysts for the experimentally simplified azide–alkyne cycloaddition. QSAR Comb. Sci. 2007, 26, 1274–1279. [Google Scholar] [CrossRef]
  42. Bergbreiter, D.E.; Hamilton, P.N.; Koshti, N.M. Self-separating homogeneous Copper (I) catalysts. J. Am. Chem. Soc. 2007, 129, 10666–10667. [Google Scholar] [CrossRef] [PubMed]
  43. Meudtner, R.M.; Hecht, S. Responsive backbones based on alternating triazole-pyridine/benzene copolymers: From helically folding polymers to metallosupramolecularly crosslinked gels. Macromol. Rapid Commun. 2008, 29, 347–351. [Google Scholar] [CrossRef]
  44. Zhang, G.; Wang, Y.; Wen, X.; Ding, C.; Li, Y. Dual-functional click-triazole: A metal chelator and immobilization linker for the construction of a heterogeneous palladium catalyst and its application for the aerobic oxidation of alcohols. Chem. Commun. 2012, 48, 2979–2981. [Google Scholar] [CrossRef] [PubMed]
  45. Astruc, D. Palladium catalysis using dendrimers: Molecular catalysts versus nanoparticles. Tetrahedron 2010, 21, 1041–1054. [Google Scholar] [CrossRef]
  46. Watanabe, W.; Maekawa, T.; Miyazaki, Y.; Kida, T.; Takeshita, K.; Mori, A. Quadruple click: A facile pathway leading to tetrakis(4-(1,2,3-triazolyl)methyl)-ethylenediamine derivatives as a new class of extracting agents for soft metal ions. Chem. Asian J. 2012, 7, 1679–1683. [Google Scholar] [CrossRef] [PubMed]
  47. Movahedi, A.; Moth-Poulsen, K.; Eklöf, J.; Nydén, M.; Kann, N. One-pot synthesis of TBTA-functionalized coordinating polymers. React. Funct. Polym. 2014, 82, 1–8. [Google Scholar] [CrossRef]
Figure 1. General scheme for the preparation of triazolic-piperazine polymers: Possible chelation methods as pendant (a), triazole (b) and integrated designs (a,b).
Figure 1. General scheme for the preparation of triazolic-piperazine polymers: Possible chelation methods as pendant (a), triazole (b) and integrated designs (a,b).
Polymers 08 00187 g001
Scheme 1. Preparation of azidomethyl poly-styrene (A) from Merrifield resin.
Scheme 1. Preparation of azidomethyl poly-styrene (A) from Merrifield resin.
Polymers 08 00187 sch001
Scheme 2. Synthesis of piperazine propargyl carbamates 2ag from N-substituted piperazine 1ag and propargyl chloroformate.
Scheme 2. Synthesis of piperazine propargyl carbamates 2ag from N-substituted piperazine 1ag and propargyl chloroformate.
Polymers 08 00187 sch002
Scheme 3. Cu(I)-catalyzed Huisgen's reaction of azidomethyl polystyrene (A) with piperazines propargyl carbamates 2ag to obtain the triazolic piperazines polymers 3ag.
Scheme 3. Cu(I)-catalyzed Huisgen's reaction of azidomethyl polystyrene (A) with piperazines propargyl carbamates 2ag to obtain the triazolic piperazines polymers 3ag.
Polymers 08 00187 sch003
Figure 2. ATR/FTIR spectra of triazolic polymers 3ag and of the starting azidomethyl polystyrene (A).
Figure 2. ATR/FTIR spectra of triazolic polymers 3ag and of the starting azidomethyl polystyrene (A).
Polymers 08 00187 g002
Figure 3. Extraction efficiencies of metal cations by the triazolic piperazine polymers 3ag (by polymer, in triplicate).
Figure 3. Extraction efficiencies of metal cations by the triazolic piperazine polymers 3ag (by polymer, in triplicate).
Polymers 08 00187 g003
Table 1. Masses, substitution and number of millimoles for 250 mg (incubation scale) of the synthesized polymer-supported triazolic piperazines 3ag.
Table 1. Masses, substitution and number of millimoles for 250 mg (incubation scale) of the synthesized polymer-supported triazolic piperazines 3ag.
Polymer aMass increase (g) bTotal mass (g)Substitution (mmol·g−1) b250 mg (mmol)
3a0.993.991.370.342
3b1.074.071.340.335
3c1.504.501.210.302
3d1.344.341.260.315
3e1.344.341.260.315
3f1.654.651.170.292
3g1.434.431.230.307
a For structures, see Scheme 3; b Based on a quantitative transformation starting from 3 g (5.46 mmol N3) of polymer A.
Table 2. Average pH before and after incubation with polymers 3ag, and pK1 for each metal ion solution.
Table 2. Average pH before and after incubation with polymers 3ag, and pK1 for each metal ion solution.
CationAnionpHi apHf bpK1 c
Fe3+Cl3.71 ± 0.013.19 ± 0.022.2
Cu2+SO42−6.90 ± 0.017.06 ± 0.617.6
Pb2+NO35.07 ± 0.015.26 ± 0.597.7
Ni2+Cl6.13 ± 0.017.36 ± 0.629.9
Zn2+SO42−6.64 ± 0.015.71 ± 0.299.5
a pH of the salt solution before incubation. Water pH was 6.66; b pH of the salt solution after incubation; c pK1 values from references included in [40].
Back to TopTop