Next Article in Journal
Highly Active Copolymerization of Ethylene and N-Acetyl-O-(ω-Alkenyl)-l-Tyrosine Ethyl Esters Catalyzed by Titanium Complex
Next Article in Special Issue
Preparation of Uniform-Sized and Dual Stimuli-Responsive Microspheres of Poly(N-Isopropylacrylamide)/Poly(Acrylic acid) with Semi-IPN Structure by One-Step Method
Previous Article in Journal
Polylactide/Montmorillonite Hybrid Latex as a Barrier Coating for Paper Applications
Previous Article in Special Issue
Polydopamine Particle as a Particulate Emulsifier
Open AccessArticle

Fabrication of Alkoxyamine-Functionalized Magnetic Core-Shell Microspheres via Reflux Precipitation Polymerization for Glycopeptide Enrichment

by Meng Yu 1,†, Yi Di 2,†, Ying Zhang 2, Yuting Zhang 1, Jia Guo 1, Haojie Lu 2,* and Changchun Wang 1,*
1
Department of Macromolecular Science, State Key Laboratory of Molecular Engineering of Polymers, Laboratory of Advanced Materials, Fudan University, Shanghai 200433, China
2
Institutes of Biomedical Sciences and Department of Chemistry, Fudan University, Shanghai 200032, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally.
Academic Editor: Guanghui Ma
Polymers 2016, 8(3), 74; https://doi.org/10.3390/polym8030074
Received: 19 January 2016 / Revised: 14 February 2016 / Accepted: 19 February 2016 / Published: 4 March 2016
(This article belongs to the Special Issue Selected Papers from ASEPFPM2015)
As a facile method to prepare hydrophilic polymeric microspheres, reflux precipitation polymerization has been widely used for preparation of polymer nanogels. In this article, we synthesized a phthalamide-protected N-aminooxy methyl acrylamide (NAMAm-p) for preparation of alkoxyamine-functionalized polymer composite microspheres via reflux precipitation polymerization. The particle size and functional group density of the composite microspheres could be adjusted by copolymerization with the second monomers, N-isopropyl acrylamide, acrylic acid or 2-hydroxyethyl methacrylate. The resultant microspheres have been characterized by TEM, FT-IR, TGA and DLS. The experimental results showed that the alkoxyamine group density of the microspheres could reach as high as 1.49 mmol/g, and these groups showed a great reactivity with ketone/aldehyde compounds. With the aid of magnetic core, the hybrid microspheres could capture and magnetically isolate glycopeptides from the digested mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio. After that, we applied the composite microspheres to profile the glycol-proteome of a normal human serum sample, 95 unique glycopeptides and 64 glycoproteins were identified with these enrichment substrates in a 5 μL of serum sample. View Full-Text
Keywords: alkoxyamine-functionalized microspheres; reflux precipitation polymerization; magnetic composite microspheres; oxime click; glycoproteins/glycopeptides enrichment alkoxyamine-functionalized microspheres; reflux precipitation polymerization; magnetic composite microspheres; oxime click; glycoproteins/glycopeptides enrichment
Show Figures

Graphical abstract

MDPI and ACS Style

Yu, M.; Di, Y.; Zhang, Y.; Zhang, Y.; Guo, J.; Lu, H.; Wang, C. Fabrication of Alkoxyamine-Functionalized Magnetic Core-Shell Microspheres via Reflux Precipitation Polymerization for Glycopeptide Enrichment. Polymers 2016, 8, 74.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop