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Polymers 2016, 8(3), 58;

Evaluation of Electrospun PCL-PIBMD Meshes Modified with Plasmid Complexes in Vitro and in Vivo

School of Chemical Engineering and Technology, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Tianjin University, Tianjin 300072, China
Tianjin University–Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Tianjin 300072, China
Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China
Institute of Biomaterial Science, Berlin Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513 Teltow, Germany
Tianjin University–Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Kantstr. 55, 14513 Teltow, Germany
Department of Physiology and Pathophysiology, Logistics University of Chinese People’s Armed Police Force, Tianjin 300162, China
Authors to whom correspondence should be addressed.
Academic Editor: Esmaiel Jabbari
Received: 23 January 2016 / Revised: 12 February 2016 / Accepted: 15 February 2016 / Published: 23 February 2016
(This article belongs to the Special Issue Polymers Applied in Tissue Engineering)
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Functional artificial vascular meshes from biodegradable polymers have been widely explored for certain tissue engineered meshes. Still, the foreign body reaction and limitation in endothelialization are challenges for such devices. Here, degradable meshes from phase-segregated multiblock copolymers consisting of poly(ε-caprolactone) (PCL) and polydepsipeptide segments are successfully prepared by electrospinning and electrospraying techniques. The pEGFP-ZNF580 plasmid microparticles (MPs-pZNF580) were loaded into the electrospun meshes to enhance endothelialization. These functional meshes were evaluated in vitro and in vivo. The adhesion and proliferation of endothelial cells on the meshes were enhanced in loaded mesh groups. Moreover, the hemocompatibility and the tissue response of the meshes were further tested. The complete tests showed that the vascular meshes modified with MPs-pZNF580 possessed satisfactory performance with an average fiber diameter of 550 ± 160 nm, tensile strength of 27 ± 3 MPa, Young’s modulus of 1. 9 ± 0.2 MPa, water contact angle of 95° ± 2°, relative cell number of 122% ± 1% after 7 days of culture, and low blood platelet adhesion as well as weak inflammatory reactions compared to control groups. View Full-Text
Keywords: electrospun meshes; microparticles; endothelialization electrospun meshes; microparticles; endothelialization

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Feng, Y.; Liu, W.; Ren, X.; Lu, W.; Guo, M.; Behl, M.; Lendlein, A.; Zhang, W. Evaluation of Electrospun PCL-PIBMD Meshes Modified with Plasmid Complexes in Vitro and in Vivo. Polymers 2016, 8, 58.

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