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Polymers 2014, 6(9), 2357-2370;

Laccase Immobilization by Chelated Metal Ion Coordination Chemistry

Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China
Author to whom correspondence should be addressed.
Received: 4 May 2014 / Revised: 28 July 2014 / Accepted: 26 August 2014 / Published: 15 September 2014
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In this work, amidoxime polyacrylonitrile (AOPAN) nanofibrous membrane was prepared by a reaction between PAN nanofibers and hydroxylamine hydrochloride. The AOPAN nanofibrous membranes were used for four metal ions (Fe3+, Cu2+, Ni2+, Cd2+) chelation under different conditions. Further, the competition of different metal ions coordinating with AOPAN nanofibrous membrane was also studied. The AOPAN chelated with individual metal ion (Fe3+, Cu2+, Ni2+, Cd2+) and also the four mixed metal ions were further used for laccase (Lac) immobilization. Compared with free laccase, the immobilized laccase showed better resistance to pH and temperature changes as well as improved storage stability. Among the four individual metal ion chelated membranes, the stability of the immobilized enzymes generally followed the order as Fe–AOPAN–Lac > Cu–AOPAN–Lac > Ni–AOPAN–Lac > Cd–AOPAN–Lac. In addition, the immobilized enzyme on the carrier of AOPAN chelated with four mixed metal ions showed the best properties. View Full-Text
Keywords: amidoxime polyacrylonitrile; metal chelation; laccase; immobilization; nanofibers amidoxime polyacrylonitrile; metal chelation; laccase; immobilization; nanofibers

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Wang, Q.; Cui, J.; Li, G.; Zhang, J.; Huang, F.; Wei, Q. Laccase Immobilization by Chelated Metal Ion Coordination Chemistry. Polymers 2014, 6, 2357-2370.

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