A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular Implant
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fabrication of the Fibrillary Scaffolds by Electrospinning
2.2. Scanning Electron Microscopy
2.3. Evaluation of the Electrospun Fiber Diameter
2.4. Drug Release Study
2.5. Hydrogel in the Polymer Tubular Structures
2.6. Cell Culturing
2.7. Immunohistochemistry
2.8. Beating Frequency
2.9. Quantification of the Markers
2.10. Cell Proliferation
2.11. Cytotoxicity Evaluation
2.12. Statistics
3. Results
3.1. Fiber Diameter Measurement
3.2. In Vitro Evaluation of the Dexamethasone (DEX) Release
3.3. Evaluation of the Cytocompatibility of the Electrospun Scaffolds
3.4. Cell Proliferation
3.5. CD31, αSMA, Col I as Markers of the HUVECs and HUVSMCs
3.6. SAA, Cx43, CD31 as Markers of the iPSC-CMs Containing Constructs
3.7. Beating Frequency
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Polymeric Solution | DEX Concentration (mg/mL) |
---|---|
PLA | 0 |
20 PLA | 20 |
40 PLA | 40 |
PLGA | 0 |
20 PLGA | 20 |
40 PLGA | 40 |
Antibody | Dilution | Supplier |
---|---|---|
Anti-α-actinin (sarcomeric), mouse monoclonal (primary) | 1:50 | Sigma-Aldrich (Saint Louis, MO, USA) |
Connexin 43 (polyclonal) (primany) | 1:50 | Thermo Fischer (Waltham, MA, USA) |
Connexin 43 monoclonal antibody (3D8A5) (primary) | 1:50 | Thermo Fischer (Waltham, MA, USA) |
Anti-CD31 (PECAM-1) mouse monoclonal (primary) | 1:100 | Sigma-Aldrich (Saint Louis, MO, USA) |
CD31 rabbit polyclonal (primary) | 1:100 | Abbiotec (Escondido, CA, USA) |
α-smooth muscle actin (monoclonal, clone 1A4) (primary) | 1:1000 | Sigma-Aldrich (Saint Louis, MO, USA) |
Anti-collagen I antibody (ab34710) (primary) | 1:200 | Abcam (Cambridge, UK) |
Alexa 594 goat anti-mouse (A11005) (secondary) | 1:400 | Thermo Fischer (Waltham, MA, USA) |
Alexa 594 goat anti-rabbit (A110012) (secondary) | 1:400 | Thermo Fischer (Waltham, MA, USA) |
Alexa 488 goat anti-mouse (A11001) (secondary) | 1:400 | Thermo Fischer (Waltham, MA, USA) |
Alexa 488 goat anti-mouse (A11008) (secondary) | 1:400 | Thermo Fischer (Waltham, MA, USA) |
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Kyriakou, S.; Acosta, S.; El Maachi, I.; Rütten, S.; Jockenhoevel, S. A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular Implant. Polymers 2023, 15, 4332. https://doi.org/10.3390/polym15214332
Kyriakou S, Acosta S, El Maachi I, Rütten S, Jockenhoevel S. A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular Implant. Polymers. 2023; 15(21):4332. https://doi.org/10.3390/polym15214332
Chicago/Turabian StyleKyriakou, Stavroula, Sergio Acosta, Ikram El Maachi, Stephan Rütten, and Stefan Jockenhoevel. 2023. "A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular Implant" Polymers 15, no. 21: 4332. https://doi.org/10.3390/polym15214332