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Article

Synthesis and In Vitro Characterization of Ascorbyl Palmitate-Loaded Solid Lipid Nanoparticles

1
Faculty of Science, Division of Molecular Biology, Department of Biology, University of Zagreb, 10 000 Zagreb, Croatia
2
Division of Materials Chemistry, Radiation Chemistry and Dosimetry Laboratory, Ruđer Bošković Institute, 10 000 Zagreb, Croatia
3
Croatian Institute of Public Health, Department of Health Ecology, 10 000 Zagreb, Croatia
4
Division of Materials Physics, Laboratory for Molecular Physics and Synthesis of New Materials, Ruđer Bošković Institute, 10 000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Academic Editor: Prashanth Asuri
Polymers 2022, 14(9), 1751; https://doi.org/10.3390/polym14091751
Received: 30 March 2022 / Revised: 15 April 2022 / Accepted: 22 April 2022 / Published: 26 April 2022
(This article belongs to the Special Issue Biomaterials for Tissue Engineering and Regeneration)
Antitumor applications of ascorbic acid (AA) and its oxidized form dehydroascorbic acid (DHA) can be quite challenging due to their instability and sensitivity to degradation in aqueous media. To overcome this obstacle, we have synthesized solid lipid nanoparticles loaded with ascorbyl palmitate (SLN-AP) with variations in proportions of the polymer Pluronic F-68. SLNs were synthesized using the hot homogenization method, characterized by measuring the particle size, polydispersity, zeta potential and visualized by TEM. To investigate the cellular uptake of the SLN, we have incorporated coumarin-6 into the same SLN formulation and followed their successful uptake for 48 h. We have tested the cytotoxicity of the SLN formulations and free ascorbate forms, AA and DHA, on HEK 293 and U2OS cell lines by MTT assay. The SLN-AP in both formulations have a cytotoxic effect at lower concentrations when compared to ascorbate applied the form of AA or DHA. Better selectivity for targeting tumor cell line was observed with 3% Pluronic F-68. The antioxidative effect of the SLN-AP was observed as early as 1 h after the treatment with a small dose of ascorbate applied (5 µM). SLN-AP formulation with 3% Pluronic F-68 needs to be further optimized as an ascorbate carrier due to its intrinsic cytotoxicity. View Full-Text
Keywords: ascorbate; ascorbyl palmitate; drug delivery; cellular uptake; nanoparticles; antitumor effect ascorbate; ascorbyl palmitate; drug delivery; cellular uptake; nanoparticles; antitumor effect
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MDPI and ACS Style

Ledinski, M.; Marić, I.; Peharec Štefanić, P.; Ladan, I.; Caput Mihalić, K.; Jurkin, T.; Gotić, M.; Urlić, I. Synthesis and In Vitro Characterization of Ascorbyl Palmitate-Loaded Solid Lipid Nanoparticles. Polymers 2022, 14, 1751. https://doi.org/10.3390/polym14091751

AMA Style

Ledinski M, Marić I, Peharec Štefanić P, Ladan I, Caput Mihalić K, Jurkin T, Gotić M, Urlić I. Synthesis and In Vitro Characterization of Ascorbyl Palmitate-Loaded Solid Lipid Nanoparticles. Polymers. 2022; 14(9):1751. https://doi.org/10.3390/polym14091751

Chicago/Turabian Style

Ledinski, Maja, Ivan Marić, Petra Peharec Štefanić, Iva Ladan, Katarina Caput Mihalić, Tanja Jurkin, Marijan Gotić, and Inga Urlić. 2022. "Synthesis and In Vitro Characterization of Ascorbyl Palmitate-Loaded Solid Lipid Nanoparticles" Polymers 14, no. 9: 1751. https://doi.org/10.3390/polym14091751

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