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Article

Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties

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Associate Laboratory i4HB-Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal
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UCIBIO—Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal
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73100 Lda., Edifício Arcis, Rua Ivone Silva, 6, 4° piso, 1050-124 Lisboa, Portugal
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Interfaculty Research Centre of Biomaterials (CEIB), University of Liège, B-4000 Liège, Belgium
*
Author to whom correspondence should be addressed.
Academic Editors: Chia-Ching Wu, Chun-Yen Liu, Masaya Yamamoto and Michiya Matsusaki
Polymers 2022, 14(3), 390; https://doi.org/10.3390/polym14030390
Received: 15 December 2021 / Revised: 8 January 2022 / Accepted: 17 January 2022 / Published: 19 January 2022
(This article belongs to the Special Issue 3D Cellular and Biomedical Applications by Polymer-Based Materials)
In this study, membrane-based methods were evaluated for the recovery of FucoPol, the fucose-rich exopolysaccharide (EPS) secreted by the bacterium Enterobacter A47, aiming at reducing the total water consumption and extraction time, while keeping a high product recovery, thus making the downstream procedure more sustainable and cost-effective. The optimized method involved ultrafiltration of the cell-free supernatant using a 30 kDa molecular weight cut-off (MWCO) membrane that allowed for a 37% reduction of the total water consumption and a 55% reduction of the extraction time, compared to the previously used method (diafiltration-ultrafiltration with a 100 kDa MWCO membrane). This change in the downstream procedure improved the product’s recovery (around 10% increase) and its purity, evidenced by the lower protein (8.2 wt%) and inorganic salts (4.0 wt%) contents of the samples (compared to 9.3 and 8.6 wt%, respectively, for the previously used method), without impacting FucoPol’s sugar and acyl groups composition, molecular mass distribution or thermal degradation profile. The biopolymer’s emulsion-forming and stabilizing capacity was also not affected (emulsification activity (EA) with olive oil, at a 2:3 ratio, of 98 ± 0% for all samples), while the rheological properties were improved (the zero-shear viscosity increased from 8.89 ± 0.62 Pa·s to 17.40 ± 0.04 Pa·s), which can be assigned to the higher purity degree of the extracted samples. These findings demonstrate a significant improvement in the downstream procedure raising FucoPol’s recovery, while reducing water consumption and operation time, key criteria in terms of process economic and environmental sustainability. Moreover, those changes improved the biopolymer’s rheological properties, known to significantly impact FucoPol’s utilization in cosmetic, pharmaceutical or food products. View Full-Text
Keywords: exopolysaccharide; fucopol; extraction; diafiltration; ultrafiltration; emulsion; rheology exopolysaccharide; fucopol; extraction; diafiltration; ultrafiltration; emulsion; rheology
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MDPI and ACS Style

Baptista, S.; Torres, C.A.V.; Sevrin, C.; Grandfils, C.; Reis, M.A.M.; Freitas, F. Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties. Polymers 2022, 14, 390. https://doi.org/10.3390/polym14030390

AMA Style

Baptista S, Torres CAV, Sevrin C, Grandfils C, Reis MAM, Freitas F. Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties. Polymers. 2022; 14(3):390. https://doi.org/10.3390/polym14030390

Chicago/Turabian Style

Baptista, Sílvia, Cristiana A. V. Torres, Chantal Sevrin, Christian Grandfils, Maria A. M. Reis, and Filomena Freitas. 2022. "Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties" Polymers 14, no. 3: 390. https://doi.org/10.3390/polym14030390

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