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Article

Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics

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Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Straße 6, 01069 Dresden, Germany
2
Organische Chemie der Polymere, Technische Universität Dresden, Mommsenstraße 4, 01062 Dresden, Germany
3
Institut für Halbleiter- und Mikrosystemtechnik, Technische Universität Dresden, Nöthnitzer Straße 64, 01187 Dresden, Germany
*
Authors to whom correspondence should be addressed.
Academic Editors: Marek Kowalczuk, Iza Radecka and Barbara Trzebicka
Polymers 2022, 14(2), 267; https://doi.org/10.3390/polym14020267
Received: 13 December 2021 / Revised: 4 January 2022 / Accepted: 5 January 2022 / Published: 10 January 2022
Stimuli-responsive hydrogels have a wide range of potential applications in microfluidics, which has drawn great attention. Double cross-linked hydrogels are very well suited for this application as they offer both stability and the required responsive behavior. Here, we report the integration of poly(N-isopropylacrylamide) (PNiPAAm) hydrogel with a permanent cross-linker (N,N′-methylenebisacrylamide, BIS) and a redox responsive reversible cross-linker (N,N′-bis(acryloyl)cystamine, BAC) into a microfluidic device through photopolymerization. Cleavage and re-formation of disulfide bonds introduced by BAC changed the cross-linking densities of the hydrogel dots, making them swell or shrink. Rheological measurements allowed for selecting hydrogels that withstand long-term shear forces present in microfluidic devices under continuous flow. Once implemented, the thiol-disulfide exchange allowed the hydrogel dots to successfully capture and release the protein bovine serum albumin (BSA). BSA was labeled with rhodamine B and functionalized with 2-(2-pyridyldithio)-ethylamine (PDA) to introduce disulfide bonds. The reversible capture and release of the protein reached an efficiency of 83.6% in release rate and could be repeated over 3 cycles within the microfluidic device. These results demonstrate that our redox-responsive hydrogel dots enable the dynamic capture and release of various different functionalized (macro)molecules (e.g., proteins and drugs) and have a great potential to be integrated into a lab-on-a-chip device for detection and/or delivery. View Full-Text
Keywords: microfluidics; disulfide bonds; redox-responsive; hydrogels; protein capture and release; swelling behaviors; mechanical properties microfluidics; disulfide bonds; redox-responsive; hydrogels; protein capture and release; swelling behaviors; mechanical properties
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MDPI and ACS Style

Jiao, C.; Obst, F.; Geisler, M.; Che, Y.; Richter, A.; Appelhans, D.; Gaitzsch, J.; Voit, B. Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics. Polymers 2022, 14, 267. https://doi.org/10.3390/polym14020267

AMA Style

Jiao C, Obst F, Geisler M, Che Y, Richter A, Appelhans D, Gaitzsch J, Voit B. Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics. Polymers. 2022; 14(2):267. https://doi.org/10.3390/polym14020267

Chicago/Turabian Style

Jiao, Chen, Franziska Obst, Martin Geisler, Yunjiao Che, Andreas Richter, Dietmar Appelhans, Jens Gaitzsch, and Brigitte Voit. 2022. "Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics" Polymers 14, no. 2: 267. https://doi.org/10.3390/polym14020267

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