Biodegradable Nanoparticles Prepared from Chitosan and Casein for Delivery of Bioactive Polysaccharides
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of CS/OJPs/CL Co-Assembled Nanoparticles
2.3. Characterization of CS/OJPs/CL Nanoparticles
2.4. pH-Responsive and Biodegradable Properties
2.5. Drug Loading and Release
2.6. Cellular Uptake of Nanoparticles
2.7. Cytotoxicity Assay
2.8. DPPH and ABTS Scavenging
2.9. Determination of Nitric Oxide
2.10. Statistical Analysis
3. Results and Discussion
3.1. Optimization of CS/OJPs/CL Assembly
3.2. Characterizatio of OJPs/CS/CL NPs
3.3. pH-Responsive and Biodegradable Properties
3.4. Drug Loading and Release
3.5. In Vitro Antioxidant Activity
3.6. Phagocytic Uptake and Cytotoxicity of Nanoparticles
3.7. Protective Effect against Ni2+-Induced Cytotoxicity and LPS-Induced Inflammation
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Mean Particle Size | Zeta Potential | Encapsulation Efficiency | |
---|---|---|---|
(nm) | (mV) | (%) | |
CS/OJPs NPs | 195.5 ± 4.6 | 1.1 ± 0.3 (pH 6.5) | 78.6 ± 1.4 |
5.8 ± 1.1 (pH 6.0) | |||
12.9 ± 2.3 (pH 5.0) | |||
CS/OJPs/CL NPs | 198.1 ± 6.8 | 0.6 ± 0.1 (pH 6.5) | 88.1 ± 0.2 |
5.5 ± 1.3 (pH 6.0) | |||
13.6 ± 2.5 (pH 5.0) |
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Lin, C.; Hsu, F.-Y.; Lin, W.-T.; Cha, C.-Y.; Ho, Y.-C.; Mi, F.-L. Biodegradable Nanoparticles Prepared from Chitosan and Casein for Delivery of Bioactive Polysaccharides. Polymers 2022, 14, 2966. https://doi.org/10.3390/polym14142966
Lin C, Hsu F-Y, Lin W-T, Cha C-Y, Ho Y-C, Mi F-L. Biodegradable Nanoparticles Prepared from Chitosan and Casein for Delivery of Bioactive Polysaccharides. Polymers. 2022; 14(14):2966. https://doi.org/10.3390/polym14142966
Chicago/Turabian StyleLin, Chi, Fang-Yu Hsu, Wei-Ting Lin, Chia-Yun Cha, Yi-Cheng Ho, and Fwu-Long Mi. 2022. "Biodegradable Nanoparticles Prepared from Chitosan and Casein for Delivery of Bioactive Polysaccharides" Polymers 14, no. 14: 2966. https://doi.org/10.3390/polym14142966