Improvement on Thermostability of Pectate Lyase and Its Potential Application to Ramie Degumming
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Prediction of Mutation Sites
2.2.2. Construction and Prokaryotic Expression of Recombinant Mutagenic Plasmid, Preparation of Cell-Free Extract
2.2.3. SDS-PAGE Analysis and Enzymatic Activity Determination
2.2.4. Thermostability of Mutants
2.2.5. Ramie Degumming through Enzymatic Method
3. Results
3.1. Selection of Mutation Sites
3.2. Heterologous Expression Analysis of Mutant Pectate Lyase
3.3. Determination of the Optimal Catalytic Temperature
3.4. Thermostability Test
3.5. Ramie Degumming Effect
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Enzymes | CK | Pel419 | V52A | Novo |
---|---|---|---|---|
Weight loss rate (%) | 4.77 ± 0.32 | 12.89 ± 0.28 | 18.77 ± 0.23 | 19.22 ± 0.21 |
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Xu, H.; Feng, X.; Yang, Q.; Zheng, K.; Yi, L.; Duan, S.; Cheng, L. Improvement on Thermostability of Pectate Lyase and Its Potential Application to Ramie Degumming. Polymers 2022, 14, 2878. https://doi.org/10.3390/polym14142878
Xu H, Feng X, Yang Q, Zheng K, Yi L, Duan S, Cheng L. Improvement on Thermostability of Pectate Lyase and Its Potential Application to Ramie Degumming. Polymers. 2022; 14(14):2878. https://doi.org/10.3390/polym14142878
Chicago/Turabian StyleXu, Huan, Xiangyuan Feng, Qi Yang, Ke Zheng, Le Yi, Shengwen Duan, and Lifeng Cheng. 2022. "Improvement on Thermostability of Pectate Lyase and Its Potential Application to Ramie Degumming" Polymers 14, no. 14: 2878. https://doi.org/10.3390/polym14142878