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Article

Synthesis and Characterization of Biocompatible Methacrylated Kefiran Hydrogels: Towards Tissue Engineering Applications

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3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal
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ICVS/3B’s–PT Government Associate Laboratory, Braga, 4805-017 Guimarães, Portugal
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Author to whom correspondence should be addressed.
Academic Editor: Hsiuying Wang
Polymers 2021, 13(8), 1342; https://doi.org/10.3390/polym13081342
Received: 2 April 2021 / Revised: 15 April 2021 / Accepted: 18 April 2021 / Published: 20 April 2021
(This article belongs to the Special Issue Biomaterials in Medical Applications)
Hydrogel application feasibility is still limited mainly due to their low mechanical strength and fragile nature. Therefore, several physical and chemical cross-linking modifications are being used to improve their properties. In this research, methacrylated Kefiran was synthesized by reacting Kefiran with methacrylic anhydride (MA). The developed MA-Kefiran was physicochemically characterized, and its biological properties evaluated by different techniques. Chemical modification of MA-Kefiran was confirmed by 1H-NMR and FTIR and GPC-SEC showed an average Mw of 793 kDa (PDI 1.3). The mechanical data obtained revealed MA-Kefiran to be a pseudoplastic fluid with an extrusion force of 11.21 ± 2.87 N. Moreover, MA-Kefiran 3D cryogels were successfully developed and fully characterized. Through micro-CT and SEM, the scaffolds revealed high porosity (85.53 ± 0.15%) and pore size (33.67 ± 3.13 μm), thick pore walls (11.92 ± 0.44 μm) and a homogeneous structure. Finally, MA-Kefiran revealed to be biocompatible by presenting no hemolytic activity and an improved cellular function of L929 cells observed through the AlamarBlue® assay. By incorporating methacrylate groups in the Kefiran polysaccharide chain, a MA-Kefiran product was developed with remarkable physical, mechanical, and biological properties, resulting in a promising hydrogel to be used in tissue engineering and regenerative medicine applications. View Full-Text
Keywords: biomedical applications; chemical cross-linking; hydrogel; Kefiran; methacrylation biomedical applications; chemical cross-linking; hydrogel; Kefiran; methacrylation
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MDPI and ACS Style

Radhouani, H.; Correia, S.; Gonçalves, C.; Reis, R.L.; Oliveira, J.M. Synthesis and Characterization of Biocompatible Methacrylated Kefiran Hydrogels: Towards Tissue Engineering Applications. Polymers 2021, 13, 1342. https://doi.org/10.3390/polym13081342

AMA Style

Radhouani H, Correia S, Gonçalves C, Reis RL, Oliveira JM. Synthesis and Characterization of Biocompatible Methacrylated Kefiran Hydrogels: Towards Tissue Engineering Applications. Polymers. 2021; 13(8):1342. https://doi.org/10.3390/polym13081342

Chicago/Turabian Style

Radhouani, Hajer, Susana Correia, Cristiana Gonçalves, Rui L. Reis, and Joaquim M. Oliveira 2021. "Synthesis and Characterization of Biocompatible Methacrylated Kefiran Hydrogels: Towards Tissue Engineering Applications" Polymers 13, no. 8: 1342. https://doi.org/10.3390/polym13081342

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