Analysis of Ionic-Exchange of Selected Elements between Novel Nano-Hydroxyapatite-Silica Added Glass Ionomer Cement and Natural Teeth

Round 1

Reviewer 1 Report

Article title "Analysis of ionic-exchange of selected elements between novel nano-hydroxyapatite-silica added glass ionomer cement and natural teeth." clearly expresses the theme of this article. This topic is of interest for the medial dental field, because dentistry is looking for the ideal material to replace the lost tooth structure. Based on literary research, most materials used today are bio-inert and have anticariogenic properties. Numerous researches have been done related to the binding mechanism between the tooth and the restoration material regarding the ion exchange in relation to time. The most relevant results were obtained with the help of energy dispersive X-rays spectrometer, results that confirm the migration of elements between the cGIC and the tooth.

The basic material used in this study was glass ionomer cement in dust and liquid state, and the chemicals used were of analytical quality. Ten premolars extracted for orthodontic and periodontal reasons were used. They were prepared for ion exchange evaluation. The images used are clear and clearly highlight the topic of interest. All the data were analysed using SPSS version 23.

The results of the mean and standard deviation of the ion concentration for the selected elements in the experimental samples recorded at different time intervals are presented in the tables. Ion-concentration of various selected elements in enamel / dentine and cGIC at various time intervals. The ion exchange between cGIC and enamel, fluorine, silicon, phosphorus, calcium, strontium and aluminium was expressed using graphs. These graphics are easy to understand and interpret. Ion exchange between nano-HA-SiO2-GIC and enamel Fluorine, silicon, phosphorus, calcium, strontium and aluminium was also shown using the graphs.

The discussions in this article highlight the following statements: ion exchange between glass ionomer cement and tooth structure requires an aqueous medium for ion exchange to take place. Strontium and aluminium ions in adjacent enamel and underlying dentin can be derived from the filler fraction inorganic of nano-HA-SiO2-GIC. The significant increase in the percentage by weight of fluorine, aluminum, calcium, phosphate, strontium and silicon in the dentin adjacent to the nano-HA-SiO2-GIC cement may indicate the transition of these elements from restoration to tooth.

In conclusion, this study confirms the ion exchange between nano-HA-SiO2-GIC and natural teeth, leading to the assumption that increased remineralisation may be possible with nano-HA-SiO2-GIC compared to cGIC because it involves elements penetrating the restoration material into tooth.

This article is a step forward for medical research and restorative dentistry.

BUT: The design of the study is inadequate. It needs artificial saliva. It is necessary to analyze these ions in artificial saliva.

Author Response

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Author Response File: Author Response.docx

Reviewer 2 Report

Dear Authors,

   In the manuscript, the authors synthesized and investigated the transfer of specific elements (calcium, phosphorus, fluoride, silica, strontium and alumina) between nano-hydroxyapatite-silica added GIC and human enamel and dentin. The authors had reconfirmed that the hypothesis for the current study that the addition of nano-hydroxyapatite-silica powder to conventional GIC enhances the chemical properties of GIC in term of ionic-exchange which induce remineralization in natural teeth. The results of this study may be relevant for progression of dental materials particularly using restorative dentistry

There are some concerns described below,

1. The EDX analysis was performed by collecting line-scans and dot-scans, please clearly describe how to determine the position to scan.

2. The photographs (Fig.1,Fig.2) of the sections do not appear sharply focus and color.     

3,There are some typos. Please carefully review the entire manuscript.

Especially abbreviated words were duplicated.

4. Please add the approval number of the ethical committee in Materials and methods section.

 

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Round 2

Reviewer 1 Report

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