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Article

Bioactivity Comparison of Electrospun PCL Mats and Liver Extracellular Matrix as Scaffolds for HepG2 Cells

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Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
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Faculty of Textile Technology, University of Zagreb, Prilaz baruna Filipovića 28a, 1000 Zagreb, Croatia
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Faculty of Metallurgy, University of Zagreb, Aleja narodnih heroja 3, 44000 Sisak, Croatia
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Department of Transfusion and Regenerative Medicine, University Hospital Centre Sestre Milosrdnice, Draškovićeva 19, 10000 Zagreb, Croatia
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Faculty of Chemical Engineering and Technology, University of Zagreb, 10000 Zagreb, Croatia
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Institute of Immunology, Rockefellerova 2, 10000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Polymers 2021, 13(2), 279; https://doi.org/10.3390/polym13020279
Received: 16 December 2020 / Revised: 12 January 2021 / Accepted: 12 January 2021 / Published: 16 January 2021
(This article belongs to the Special Issue Polymeric Materials as Scaffolds for Tissue Engineering)
Cells grown on bioactive matrices have immensely advanced many aspects of biomedical research related to drug delivery and tissue engineering. Our main objective was to perform simple evaluation of the structural and biotic qualities of cell scaffolds made of affordable biomaterials for liver cell line (HepG2) cultivation in vitro. In this work the electrospun matrix made of synthetic polyester poly(ε-caprolactone) (PCL) was compared with the natural protein-based extracellular matrix isolated from porcine liver (ECM). Mechanical and structural analysis showed that ECM was about 12 times less resistant to tensile stress while it had significantly larger pore size and twice smaller water contact angle than PCL. Bioactivity assessment included comparison of cell growth and transfection efficiency on cell-seeded scaffolds. Despite the differences in composition and structure between the two respective matrices, the rate of cell spreading and the percentage of transfected cells on both scaffolds were fairly comparable. These results suggest that in an attempt to produce simple, cell carrying structures that adequately simulate the natural scaffold, one can rely on PCL electrospun mats. View Full-Text
Keywords: PCL; extracellular matrix; ECM; HepG2 cells; electrospinning PCL; extracellular matrix; ECM; HepG2 cells; electrospinning
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MDPI and ACS Style

Slivac, I.; Zdraveva, E.; Ivančić, F.; Žunar, B.; Holjevac Grgurić, T.; Gaurina Srček, V.; Svetec, I.-K.; Dolenec, T.; Bajsić, E.G.; Tominac Trcin, M.; Mijović, B. Bioactivity Comparison of Electrospun PCL Mats and Liver Extracellular Matrix as Scaffolds for HepG2 Cells. Polymers 2021, 13, 279. https://doi.org/10.3390/polym13020279

AMA Style

Slivac I, Zdraveva E, Ivančić F, Žunar B, Holjevac Grgurić T, Gaurina Srček V, Svetec I-K, Dolenec T, Bajsić EG, Tominac Trcin M, Mijović B. Bioactivity Comparison of Electrospun PCL Mats and Liver Extracellular Matrix as Scaffolds for HepG2 Cells. Polymers. 2021; 13(2):279. https://doi.org/10.3390/polym13020279

Chicago/Turabian Style

Slivac, Igor, Emilija Zdraveva, Fran Ivančić, Bojan Žunar, Tamara Holjevac Grgurić, Višnja Gaurina Srček, Ivan-Krešimir Svetec, Tamara Dolenec, Emi G. Bajsić, Mirna Tominac Trcin, and Budimir Mijović. 2021. "Bioactivity Comparison of Electrospun PCL Mats and Liver Extracellular Matrix as Scaffolds for HepG2 Cells" Polymers 13, no. 2: 279. https://doi.org/10.3390/polym13020279

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