In Vitro Interaction of Doxorubicin-Loaded Silk Sericin Nanocarriers with MCF-7 Breast Cancer Cells Leads to DNA Damage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Silk Sericin Nanoparticles
2.3. Drug Loading in Sericin Nanoparticles
2.4. Drug Release Behavior
2.5. Characterization Methods
2.5.1. FTIR–ATR Analysis
2.5.2. Morphological Characterization
2.5.3. Dynamic Light Scattering (DLS)
2.5.4. Conformational Analysis by Circular Dichroism (CD)
2.6. In Vitro Biological Evaluation of Free and Doxorubicin-Loaded Sericin Nanocarriers
2.6.1. Cell Culture Model
2.6.2. Cell Viability Assay
2.6.3. Cytoskeleton Investigation and DAPI staining
2.6.4. Measurement of DNA Damage by Comet Assay
2.6.5. Statistical Analysis
3. Results and Discussions
3.1. FTIR–ATR Analysis
3.2. Drug Release Behavior
3.2.1. Neutral Medium
3.2.2. Acidic Medium
3.2.3. Enzymatic Media
3.3. Morphological Characterization
3.3.1. SEM Analysis
3.3.2. TEM Analysis
3.4. Dynamic Light Scattering, Zeta Potential, and Isoelectric Point
Molecular Weight Evaluation by DLS
3.5. Conformational Analysis by Circular Dichroism (CD)
3.6. In Vitro Antitumor Activity Evaluation of DOX-Loaded Sericin Nanocarriers
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Radu, I.-C.; Zaharia, C.; Hudiță, A.; Tanasă, E.; Ginghină, O.; Marin, M.; Gălățeanu, B.; Costache, M. In Vitro Interaction of Doxorubicin-Loaded Silk Sericin Nanocarriers with MCF-7 Breast Cancer Cells Leads to DNA Damage. Polymers 2021, 13, 2047. https://doi.org/10.3390/polym13132047
Radu I-C, Zaharia C, Hudiță A, Tanasă E, Ginghină O, Marin M, Gălățeanu B, Costache M. In Vitro Interaction of Doxorubicin-Loaded Silk Sericin Nanocarriers with MCF-7 Breast Cancer Cells Leads to DNA Damage. Polymers. 2021; 13(13):2047. https://doi.org/10.3390/polym13132047
Chicago/Turabian StyleRadu, Ionuț-Cristian, Cătălin Zaharia, Ariana Hudiță, Eugenia Tanasă, Octav Ginghină, Minodora Marin, Bianca Gălățeanu, and Marieta Costache. 2021. "In Vitro Interaction of Doxorubicin-Loaded Silk Sericin Nanocarriers with MCF-7 Breast Cancer Cells Leads to DNA Damage" Polymers 13, no. 13: 2047. https://doi.org/10.3390/polym13132047