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Open AccessArticle

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

1
The Institute of Immunology, Rockefellerova ul. 2, 10000 Zagreb, Croatia
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Faculty of Textile Technology, University of Zagreb, Prilaz baruna Filipovića 28a, 10000 Zagreb, Croatia
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Department of Transfusion and Regenerative Medicine, Sestre Milosrdnice University Hospital Center, Draškovićeva 19, 10000 Zagreb, Croatia
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Department of Microbiology, Croatian Institute for Transfusion Medicine, Petrova 3, 10000 Zagreb, Croatia
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Medical Faculty of Rijeka, University Eye Hospital Svjetlost, Vjekoslava Heinzela 39, 10000 Zagreb, Croatia
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School of Medicine, University of Zagreb, Šalata 2, 10000 Zagreb, Croatia
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Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
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Faculty of Metallurgy, University of Zagreb, Aleja narodnih heroja 3, 44000 Sisak, Croatia
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Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia
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Forensic Science Centre, Ivan Vučetić“, Ilica 335, 10000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Polymers 2020, 12(8), 1758; https://doi.org/10.3390/polym12081758
Received: 30 June 2020 / Revised: 23 July 2020 / Accepted: 31 July 2020 / Published: 6 August 2020
(This article belongs to the Special Issue Electro-Fluid Dynamic Technologies for Bio-Applications)
Limbal Stem Cell Deficiency (LSCD) is a very serious and painful disease that often results in impaired vision. Cultivation of limbal stem cells for clinical application is usually performed on carriers such as amniotic membrane or surgical fibrin gel. Transplantation of these grafts is associated with the risk of local postoperative infection that can destroy the graft and devoid therapeutic benefit. For this reason, electrospun scaffolds are good alternatives, as proven to mimic the natural cells surroundings, while their fabrication technique is versatile with regard to polymer functionalization and scaffolds architecture. This study considers the development of poly(ε-caprolactone) (PCL) immune-compatible and biodegradable electrospun scaffolds, comprising cefuroxime (CF) or titanium dioxide (TiO2) active components, that provide both bactericidal activity against eye infections and support of limbal stem cells growth in vitro. The PCL/CF scaffolds were prepared by blend electrospinning, while functionalization with the TiO2 particles was performed by ultrasonic post-processing treatment. The fabricated scaffolds were evaluated in regard to their physical structure, wetting ability, static and dynamic mechanical behaviour, antimicrobial efficiency and drug release, through scanning electron microscopy, water contact angle measurement, tensile testing and dynamic mechanical analysis, antimicrobial tests and UV-Vis spectroscopy, respectively. Human limbal stem cells, isolated from surgical remains of human cadaveric cornea, were cultured on the PCL/CF and PCL/TiO2 scaffolds and further identified through immunocytochemistry in terms of cell type thus were stained against p63 marker for limbal stem cells, a nuclear transcription factor and cytokeratin 3 (CK3), a corneal epithelial differentiation marker. The electrospun PCL/CF and PCL/TiO2 successfully supported the adhesion, proliferation and differentiation of the cultivated limbal cells and provided the antimicrobial effect against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. View Full-Text
Keywords: scaffolds; tissue engineering; polycaprolactone; electrospinning; titanium dioxide; cefuroxime; limbal stem cell deficiency; antimicrobial activity scaffolds; tissue engineering; polycaprolactone; electrospinning; titanium dioxide; cefuroxime; limbal stem cell deficiency; antimicrobial activity
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MDPI and ACS Style

Tominac Trcin, M.; Zdraveva, E.; Dolenec, T.; Vrgoč Zimić, I.; Bujić Mihica, M.; Batarilo, I.; Dekaris, I.; Blažević, V.; Slivac, I.; Holjevac Grgurić, T.; Bajsić, E.G.; Markov, K.; Čanak, I.; Kuzmić, S.; Tarbuk, A.; Tomljenović, A.; Mrkonjić, N.; Mijović, B. Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells. Polymers 2020, 12, 1758. https://doi.org/10.3390/polym12081758

AMA Style

Tominac Trcin M, Zdraveva E, Dolenec T, Vrgoč Zimić I, Bujić Mihica M, Batarilo I, Dekaris I, Blažević V, Slivac I, Holjevac Grgurić T, Bajsić EG, Markov K, Čanak I, Kuzmić S, Tarbuk A, Tomljenović A, Mrkonjić N, Mijović B. Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells. Polymers. 2020; 12(8):1758. https://doi.org/10.3390/polym12081758

Chicago/Turabian Style

Tominac Trcin, Mirna; Zdraveva, Emilija; Dolenec, Tamara; Vrgoč Zimić, Ivana; Bujić Mihica, Marina; Batarilo, Ivanka; Dekaris, Iva; Blažević, Valentina; Slivac, Igor; Holjevac Grgurić, Tamara; Bajsić, Emi G.; Markov, Ksenija; Čanak, Iva; Kuzmić, Sunčica; Tarbuk, Anita; Tomljenović, Antoneta; Mrkonjić, Nikolina; Mijović, Budimir. 2020. "Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells" Polymers 12, no. 8: 1758. https://doi.org/10.3390/polym12081758

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