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Open AccessArticle

Rational Design of Amphiphilic Diblock Copolymer/MWCNT Surface Modifiers and Their Application for Direct Electrochemical Sensing of DNA

1
Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie Gory 1/3, 119991 Moscow, Russia
2
V.N. Orekhovich Institute of Biomedical Chemistry, 119121 Moscow, Russia
3
Pirogov Russian National Research Medical University, 117997 Moscow, Russia
4
Institute of Organic Chemistry and Macromolecular Chemistry (IOMC), Friedrich-Schiller-University Jena, D-07743 Jena, Germany
5
Jena Center for Soft Matter (JCSM), Friedrich-Schiller-University Jena, D-07743 Jena, Germany
6
Center for Energy and Environmental Chemistry (CEEC), Friedrich-Schiller-University Jena, D-07743 Jena, Germany
*
Author to whom correspondence should be addressed.
Polymers 2020, 12(7), 1514; https://doi.org/10.3390/polym12071514
Received: 31 May 2020 / Revised: 23 June 2020 / Accepted: 3 July 2020 / Published: 8 July 2020
(This article belongs to the Special Issue Polyelectrolytes and Interpolyelectrolyte Complexes)
We demonstrate the application of amphiphilic ionic poly(n-butylmethacrylate)-block- poly(2-(dimethylamino)ethyl methacrylate) diblock copolymers (PnBMA40-b-PDMAEMA40, PnBMA40-b-PDMAEMA120, PnBMA70-b-PDMAEMA120) for dispersing multiwalled carbon nanotubes (MWCNTs) in aqueous media, a subsequent efficient surface modification of screen-printed electrodes (SPEs), and the application of the modified SPEs for DNA electrochemistry. Stable and fine aqueous dispersions of MWCNTs were obtained with PnBMAx-b-PDMAEMAy diblock copolymers, regardless of the structure of the copolymer and the amount of MWCNTs in the dispersions. The effect of the diblock copolymer structure was important when the dispersions of MWCNTs were deposited as modifying layers on surfaces of SPEs, resulting in considerable increases of the electroactive surface areas and great acceleration of the electron transfer rate. The SPE/(PnBMAx-b-PDMAEMAy + MWCNT) constructs were further exploited for direct electrochemical oxidation of the guanine (G) and adenine (A) residues in a model salmon sperm double-stranded DNA (dsDNA). Two well-defined irreversible oxidation peaks were observed at about +600 and +900 mV, corresponding to the electrochemical oxidation of G and A residues, respectively. A multi-parametric optimization of dsDNA electrochemistry enables one to get the limits of detection (LOD) as low as 5 μg/mL (0.25 μM) and 1 μg/mL (0.05 μM) for G and A residues, respectively. The achieved sensitivity of DNA assay enables quantification of the A and G residues of dsDNA in the presence of human serum and DNA in isolated human leukocytes. View Full-Text
Keywords: MWCNT dispersion; amphiphilic ionic diblock copolymer; dsDNA; leukocyte DNA; adenine; guanine; DNA electroanalysis; dsDNA direct electrochemical oxidation MWCNT dispersion; amphiphilic ionic diblock copolymer; dsDNA; leukocyte DNA; adenine; guanine; DNA electroanalysis; dsDNA direct electrochemical oxidation
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MDPI and ACS Style

Sigolaeva, L.V.; Bulko, T.V.; Konyakhina, A.Y..; Kuzikov, A.V.; Masamrekh, R.A.; Max, J.B.; Köhler, M.; Schacher, F.H.; Pergushov, D.V.; Shumyantseva, V.V. Rational Design of Amphiphilic Diblock Copolymer/MWCNT Surface Modifiers and Their Application for Direct Electrochemical Sensing of DNA. Polymers 2020, 12, 1514.

AMA Style

Sigolaeva LV, Bulko TV, Konyakhina AY, Kuzikov AV, Masamrekh RA, Max JB, Köhler M, Schacher FH, Pergushov DV, Shumyantseva VV. Rational Design of Amphiphilic Diblock Copolymer/MWCNT Surface Modifiers and Their Application for Direct Electrochemical Sensing of DNA. Polymers. 2020; 12(7):1514.

Chicago/Turabian Style

Sigolaeva, Larisa V.; Bulko, Tatiana V.; Konyakhina, Apollinariya Y..; Kuzikov, Alexey V.; Masamrekh, Rami A.; Max, Johannes B.; Köhler, Moritz; Schacher, Felix H.; Pergushov, Dmitry V.; Shumyantseva, Victoria V. 2020. "Rational Design of Amphiphilic Diblock Copolymer/MWCNT Surface Modifiers and Their Application for Direct Electrochemical Sensing of DNA" Polymers 12, no. 7: 1514.

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