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Open AccessArticle

Mucoadhesive Bletilla striata Polysaccharide-Based Artificial Tears to Relieve Symptoms and Inflammation in Rabbit with Dry Eyes Syndrome

1
Graduate Institute of Biomedical Engineering, National Taiwan University, No.49, Fanglan Road, Daan District, Taipei 10051, Taiwan
2
Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, No. 250 Wu-Xing Street, Taipei 11031, Taiwan
3
Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli County 35053, Taiwan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Polymers 2020, 12(7), 1465; https://doi.org/10.3390/polym12071465
Received: 25 April 2020 / Revised: 13 June 2020 / Accepted: 27 June 2020 / Published: 30 June 2020
(This article belongs to the Special Issue Medical Application of Polymer-Based Composites)
Dry eye syndrome (DES) is a multifactorial disorder of the ocular surface affecting many people all over the world. However, there have been many therapeutic advancements for the treatment of DES, substantial long-term treatment remains a challenge. Natural plant-based polysaccharides have gained much importance in the field of tissue engineering for their excellent biocompatibility and unique physical properties. In this study, polysaccharides from a Chinese ground orchid, Bletilla striata, were successfully extracted and incorporated into the artificial tears for DES treatment due to its anti-inflammatory and mucoadhesive properties. The examination for physical properties such as refractive index, pH, viscosity and osmolality of the Bletilla striata polysaccharide (BSP) artificial tears fabricated in this study showed that it was in close association with that of the natural human tears. The reactive oxygen species (ROS) level and inflammatory gene expression tested in human corneal epithelium cells (HCECs) indicated that the low BSP concentrations (0.01–0.1% v/v) could effectively reduce inflammatory cytokines (TNF, IL8) and ROS levels in HCECs, respectively. Longer retention of the BSP-formulated artificial tears on the ocular surface is due to the mucoadhesive nature of BSP allowing lasting lubrication. Additionally, a rabbit’s DES model was created to evaluate the effect of BSP for treating dry eye. Schirmer test results exhibited the effectiveness of 0.1% (v/v) BSP-containing artificial tears in enhancing the tear volume in DES rabbits. This work combines the effectiveness of artificial tears and anti-inflammatory herb extract (BSP) to moisturize ocular surface and to relieve the inflammatory condition in DES rabbit, which further shows great potential of BSP in treating ocular surface diseases like DES in clinics in the future. View Full-Text
Keywords: Bletilla striata polysaccharide; dry eye syndrome; artificial tear; anti-inflammatory Bletilla striata polysaccharide; dry eye syndrome; artificial tear; anti-inflammatory
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MDPI and ACS Style

Thacker, M.; Tseng, C.-L.; Chang, C.-Y.; Jakfar, S.; Chen, H.Y.; Lin, F.-H. Mucoadhesive Bletilla striata Polysaccharide-Based Artificial Tears to Relieve Symptoms and Inflammation in Rabbit with Dry Eyes Syndrome. Polymers 2020, 12, 1465.

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