Facile Mixing of Phospholipids Promotes Self-Assembly of Low-Molecular-Weight Biodegradable Block Co-Polymers into Functional Vesicular Architectures
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Large Unilamellar Vesicles (LUVs)
2.3. Dynamic Light Scattering (DLS)
2.4. Cryogenic-Transmission Electron Microscopy (Cryo-TEM)
2.5. Calcein Leakage Assay
2.6. Preparation of Giant Unilamellar Vesicles (GUVs)
2.7. Wide-Field Deconvolution Microscopy
2.8. Phospholipase A2 Enzymatic Assay
3. Results and Discussion
3.1. Selection of Block Co-Polymers (BCPs)
3.2. Formation of Hybrid POPC/BCP Large Unilamellar Vesicles
3.3. Surface Topography of POPC/BCP Giant Unilamellar Vesicles
3.4. Surface Topography of Hybrid POPC/BCP/Cholesterol Giant Unilamellar Vesicles
3.5. Enzymatic Activity on Hybrid POPC/BCP Large Unilamellar Vesicles
3.6. Enzymatic Activity on Hybrid POPC/BCP Giant Unilamellar Vesicles
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Formulation. | Composition | POPC:BCP (mol:mol) | Mol. Wt (g/mol) | fhydrophilic | PDI | ||
---|---|---|---|---|---|---|---|
Mn | MA | MB | |||||
Lipid | |||||||
POPC | POPC | 1:0 | 760 | ||||
BCP | |||||||
PCL0.7 | PEG12-PCL6 | 0:1 | 1250 | 550 | 700 | 0.44 | 1.13 |
PCL1.1 | PEG12-PCL9 | 0:1 | 1650 | 550 | 1100 | 0.33 | 1.17 |
PLA2.75 | PEG16-PLA38 | 0:1 | 3450 | 700 | 2750 | 0.20 | 1.12 |
PBD1.2 | PEG13-PBD22 | 0:1 | 1800 | 600 | 1200 | 0.33 | 1.09 |
Hybrid (POPC/BCP or POPC/BCP/Ch) | |||||||
POPC/PCL0.7 | POPC: PEG12-PCL6 | 1:1 | |||||
POPC/PCL1.1 | POPC: PEG12-PCL9 | 1:1 | |||||
POPC/PLA2.75 | POPC: PEG16-PLA38 | 1:1 | |||||
POPC/PBD1.2 | POPC: PEG13-PBD22 | 1:1 | |||||
POPC/PCL0.7/Ch | POPC: PEG12-PCL6: Cholesterol | 1:1:1 | |||||
POPC/PCL1.1/Ch | POPC: PEG12-PCL9: Cholesterol | 1:1:1 | |||||
POPC/PLA2.75/Ch | POPC: PEG16-PLA38: Cholesterol | 1:1:1 | |||||
POPC/PBD1.2/Ch | POPC: PEG13-PBD22: Cholesterol | 1:1:1 |
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Khan, A.K.; Ho, J.C.S.; Roy, S.; Liedberg, B.; Nallani, M. Facile Mixing of Phospholipids Promotes Self-Assembly of Low-Molecular-Weight Biodegradable Block Co-Polymers into Functional Vesicular Architectures. Polymers 2020, 12, 979. https://doi.org/10.3390/polym12040979
Khan AK, Ho JCS, Roy S, Liedberg B, Nallani M. Facile Mixing of Phospholipids Promotes Self-Assembly of Low-Molecular-Weight Biodegradable Block Co-Polymers into Functional Vesicular Architectures. Polymers. 2020; 12(4):979. https://doi.org/10.3390/polym12040979
Chicago/Turabian StyleKhan, Amit Kumar, James C. S. Ho, Susmita Roy, Bo Liedberg, and Madhavan Nallani. 2020. "Facile Mixing of Phospholipids Promotes Self-Assembly of Low-Molecular-Weight Biodegradable Block Co-Polymers into Functional Vesicular Architectures" Polymers 12, no. 4: 979. https://doi.org/10.3390/polym12040979