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Open AccessArticle

The Mechanical Properties, Secondary Structure, and Osteogenic Activity of Photopolymerized Fibroin

1
Biological Faculty, Lomonosov Moscow State University, 119234 Moscow, Russia
2
JSC Efferon, 143026 Moscow, Russia
3
Regional Research and Clinical Institute (“MONIKI”), 129110 Moscow, Russia
4
A. N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, 119334 Moscow, Russia
5
Institute for Regenerative Medicine, Sechenov University, 119991 Moscow, Russia
6
Semenov Institute of Chemical Physics Russian Academy of Sciences, 119991 Moscow, Russia
7
Blokhin National Medical Research Center of Oncology, 115478 Moscow, Russia
8
Institute of Gene Biology, Russian Academy of Sciences, 119991 Moscow, Russia
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Polymers 2020, 12(3), 646; https://doi.org/10.3390/polym12030646
Received: 1 February 2020 / Revised: 2 March 2020 / Accepted: 10 March 2020 / Published: 12 March 2020
(This article belongs to the Special Issue Medical Application of Polymer-Based Composites)
Previously, we have described the preparation of a novel fibroin methacrylamide (FbMA), a polymer network with improved functionality, capable of photocrosslinking into Fb hydrogels with elevated stiffness. However, it was unclear how this new functionality affects the structure of the material and its beta-sheet-associated crystallinity. Here, we show that the proposed method of Fb methacrylation does not disturb the protein’s ability to self-aggregate into the stable beta-sheet-based crystalline domains. Fourier transform infrared spectroscopy (FTIR) shows that, although the precursor ethanol-untreated Fb films exhibited a slightly higher degree of beta-sheet content than the FbMA films (46.9% for Fb-F-aq and 41.5% for FbMA-F-aq), both materials could equally achieve the highest possible beta-sheet content after ethanol treatment (49.8% for Fb-F-et and 49.0% for FbMA-F-et). The elasticity modulus for the FbMA-F-et films was twofold higher than that of the Fb-F-et as measured by the uniaxial tension (130 ± 1 MPa vs. 64 ± 6 MPa), and 1.4 times higher (51 ± 11 MPa vs. 36 ± 4 MPa) as measured by atomic force microscopy. The culturing of human MG63 osteoblast-like cells on Fb-F-et, FbMA-F-et-w/oUV, and FbMA-F-et substrates revealed that the photocrosslinking-induced increment of stiffness increases the area covered by the cells, rearrangement of actin cytoskeleton, and vinculin distribution in focal contacts, altogether enhancing the osteoinductive activity of the substrate. View Full-Text
Keywords: silk fibroin; methacrylated silk fibroin; tissue engineering; photocrosslinking; osteogenic differentiation silk fibroin; methacrylated silk fibroin; tissue engineering; photocrosslinking; osteogenic differentiation
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Bessonov, I.; Moysenovich, A.; Arkhipova, A.; Ezernitskaya, M.; Efremov, Y.; Solodilov, V.; Timashev, P.; Shaytan, K.; Shtil, A.; Moisenovich, M. The Mechanical Properties, Secondary Structure, and Osteogenic Activity of Photopolymerized Fibroin. Polymers 2020, 12, 646.

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