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Open AccessArticle

Hemostatic Enhancement via Chitosan Is Independent of Classical Clotting Pathways—A Quantitative Study

1
Institute of Applied Mechanics, National Taiwan University, Taipei 10617, Taiwan
2
Department of Traumatology, National Taiwan University Hospital, Taipei 10002, Taiwan
3
Department of Multimedia and Game Science, Chung Chou University of Science and Technology, Changhua 51003, Taiwan
4
CoreLeader Biotech Co., Ltd., 19F., No. 100, Sec. 1 Xintai 5th Rd., Xizhi Dist., New Taipei City 22102, Taiwan
*
Author to whom correspondence should be addressed.
Polymers 2020, 12(10), 2391; https://doi.org/10.3390/polym12102391
Received: 7 September 2020 / Revised: 13 October 2020 / Accepted: 13 October 2020 / Published: 17 October 2020
(This article belongs to the Special Issue Functional Chitosan-Based Composites II)
Hemostasis is a process causing bleeding to stop, and it is known from the literature that hemostasis can be enhanced using chitosan on wound gauzes. We proposed here a continuous flow-through device, with the test blood flowing through the gauze sample at a constant flow rate and the pressure drop across the gauze measured, for assessing the hemostatic performance of the gauze. Experiments were performed using the device with both whole blood and washed blood (with clotting factors and platelets removed from the whole blood), and their results agree with each other within 10% discrepancy, indicating quantitatively that hemostatic enhancement via chitosan is essentially independent of classical clotting pathways, which was demonstrated qualitatively through animal tests in the literature. The proposed device and method can be applied for evaluating quantitatively the hemostatic performance of various gauzes in a flowing blood environment (in comparison with static tests) with less test blood (20–60% less, in comparison with that of a flow-through device driven by a constant pressure gradient), and are thus, helpful for designing better wound gauzes. In particular, it is effective to enhance the hemostatic performance further (additional 30%) through acidification (changing the amino group to the ammonium group) of the gauze for chitosan-based wound gauzes. View Full-Text
Keywords: chitosan; classical clotting pathways; flow-through device; hemostasis; wound gauzes chitosan; classical clotting pathways; flow-through device; hemostasis; wound gauzes
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MDPI and ACS Style

Chen, K.-Y.; Chen, Y.-C.; Lin, T.-H.; Yang, C.-Y.; Kuo, Y.-W.; Lei, U. Hemostatic Enhancement via Chitosan Is Independent of Classical Clotting Pathways—A Quantitative Study. Polymers 2020, 12, 2391.

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