Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System

Yu, JunJie; Lee, SuJeong; Choi, Sunkyung; Kim, Kee K.; Ryu, Bokyeong; Kim, C-Yoon; Jung, Cho-Rok; Min, Byoung-Hyun; Xin, Yuan-Zhu; Park, Su A; Kim, Wandoo; Lee, Donghyun; Lee, JunHee

doi:10.3390/polym12102203

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Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System

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JunHee Lee

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Department of Nature-Inspired System and Application, Korea Institute of Machinery & Materials, 156 Gajeongbuk-Ro, Yuseong-Gu, Daejeon 34103, Korea

Department of Biomedical Engineering, School of Integrative Engineering, Chung-Ang University, 221 Heukseok-Dong, Dongjak-Gu, Seoul 156-756, Korea

Medical Device Convergence Center, Konyang University Hospital, 158 Gwanjedong-Ro, Seo-Gu, Daejeon 35365, Korea

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Department of Biochemistry, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea

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Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Korea

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Department of Medicine, School of Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea

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Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-Gu, Daejeon 34141, Korea

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Department of Orthopedic Surgery, School of Medicine, Ajou University, 206 World Cup-ro, Yeongtonggu, Suwon 16499, Korea

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Department of Engineering Mechanics, School of Mechanical and Aerospace Engineering, Jilin University, No. 5988, Renmin Street, Changchun 130025, China

Authors to whom correspondence should be addressed.

Polymers 2020, 12(10), 2203; https://doi.org/10.3390/polym12102203

Received: 11 August 2020 / Revised: 14 September 2020 / Accepted: 23 September 2020 / Published: 25 September 2020

(This article belongs to the Special Issue Advanced Polymeric Biomaterials for Tissue Engineering)

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Abstract

Osteochondral defects, including damage to both the articular cartilage and the subchondral bone, are challenging to repair. Although many technological advancements have been made in recent years, there are technical difficulties in the engineering of cartilage and bone layers, simultaneously. Moreover, there is a great need for a valuable in vitro platform enabling the assessment of osteochondral tissues to reduce pre-operative risk. Three-dimensional (3D) bioprinting systems may be a promising approach for fabricating human tissues and organs. Here, we aimed to develop a polycaprolactone (PCL)/alginate bipartite hybrid scaffold using a multihead 3D bioprinting system. The hybrid scaffold was composed of PCL, which could improve the mechanical properties of the construct, and alginate, encapsulating progenitor cells that could differentiate into cartilage and bone. To differentiate the bipartite hybrid scaffold into osteochondral tissue, a polydimethylsiloxane coculture system for osteochondral tissue (PCSOT) was designed and developed. Based on evaluation of the biological performance of the novel hybrid scaffold, the PCL/alginate bipartite scaffold was successfully fabricated; importantly, our findings suggest that this PCSOT system may be applicable as an in vitro platform for osteochondral tissue engineering. View Full-Text

Keywords: progenitor cell; three-dimensional bioprinting system; hybrid scaffold; osteochondral tissue

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MDPI and ACS Style

Yu, J.; Lee, S.; Choi, S.; Kim, K.K.; Ryu, B.; Kim, C.-Y.; Jung, C.-R.; Min, B.-H.; Xin, Y.-Z.; Park, S.A.; Kim, W.; Lee, D.; Lee, J. Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System. Polymers 2020, 12, 2203. https://doi.org/10.3390/polym12102203

AMA Style

Yu J, Lee S, Choi S, Kim KK, Ryu B, Kim C-Y, Jung C-R, Min B-H, Xin Y-Z, Park SA, Kim W, Lee D, Lee J. Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System. Polymers. 2020; 12(10):2203. https://doi.org/10.3390/polym12102203

Chicago/Turabian Style

Yu, JunJie, SuJeong Lee, Sunkyung Choi, Kee K. Kim, Bokyeong Ryu, C-Yoon Kim, Cho-Rok Jung, Byoung-Hyun Min, Yuan-Zhu Xin, Su A. Park, Wandoo Kim, Donghyun Lee, and JunHee Lee. 2020. "Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System" Polymers 12, no. 10: 2203. https://doi.org/10.3390/polym12102203

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