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Article

Poly-L-lysine as an Effective and Safe Desquamation Inducer of Urinary Bladder Epithelium

1
University of Ljubljana, Faculty of Pharmacy, Askerceva cesta 7, 1000 Ljubljana, Slovenia
2
University of Ljubljana, Faculty of Medicine, Institute of Cell Biology, Vrazov trg 2, 1000 Ljubljana, Slovenia
*
Author to whom correspondence should be addressed.
Polymers 2019, 11(9), 1506; https://doi.org/10.3390/polym11091506
Received: 8 July 2019 / Revised: 13 September 2019 / Accepted: 14 September 2019 / Published: 16 September 2019
Induced desquamation of urinary bladder epithelial cells, also called urothelial cells, is frequently used in studies of bladder epithelial regeneration and also in treating recurrent bacterial cystitis. Positively charged polymer chitosan is known to cause large-scale desquamation of terminally differentiated urothelial cells called umbrella cells. Aiming to compare the desquamation ability of another polycation poly-L-lysine, we studied the effect of this polymer on the functional and structural integrity of the urothelium in ex vivo and in vivo experiments. The urothelium was analyzed by measuring transepithelial electrical resistance, and the structural changes of its luminal surface were analyzed with scanning electron microscopy. The results revealed a selective and concentration-dependent desquamation effect of poly-L-lysine on superficial urothelial cells followed by quick regeneration of the urothelium, which functionally and structurally recovers in 2 to 3 h after poly-L-lysine–induced injury. Poly-L-lysine was thus proven to be a promising polymer to be used when desquamation of urothelial cells is required in basic and potentially clinical studies. View Full-Text
Keywords: poly-l-lysine; urothelium; transepithelial electrical resistance; cell desquamation; ex vivo; in vivo poly-l-lysine; urothelium; transepithelial electrical resistance; cell desquamation; ex vivo; in vivo
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MDPI and ACS Style

Kerec Kos, M.; Veranič, P.; Erman, A. Poly-L-lysine as an Effective and Safe Desquamation Inducer of Urinary Bladder Epithelium. Polymers 2019, 11, 1506. https://doi.org/10.3390/polym11091506

AMA Style

Kerec Kos M, Veranič P, Erman A. Poly-L-lysine as an Effective and Safe Desquamation Inducer of Urinary Bladder Epithelium. Polymers. 2019; 11(9):1506. https://doi.org/10.3390/polym11091506

Chicago/Turabian Style

Kerec Kos, Mojca, Peter Veranič, and Andreja Erman. 2019. "Poly-L-lysine as an Effective and Safe Desquamation Inducer of Urinary Bladder Epithelium" Polymers 11, no. 9: 1506. https://doi.org/10.3390/polym11091506

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