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Article

Tunable Composition of Dynamic Non-Viral Vectors over the DNA Polyplex Formation and Nucleic Acid Transfection

“Petru Poni” Institute of Macromolecular Chemistry, Romanian Academy, Centre of Advanced Research in Bionanoconjugates and Biopolymers, Grigore Ghica Voda Alley, 41 A, 700487 Iasi, Romania
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Polymers 2019, 11(8), 1313; https://doi.org/10.3390/polym11081313
Received: 2 July 2019 / Revised: 25 July 2019 / Accepted: 4 August 2019 / Published: 6 August 2019
(This article belongs to the Special Issue Polymeric Colloidal Systems in Nanomedicine)
Polyethylene glycol (PEG) functionalization of non-viral vectors represents a powerful tool through the formation of an overall surface charge shielding ability, which is fundamental for efficient nucleic acid delivery systems. The degree of non-viral vector PEGylation and the molecular weight of utilized PEG is crucial since the excessive use of PEG units may lead to a considerable reduction of the DNA-binding capacity and, subsequently, in a reduction of in vitro transfection efficiency. Herein, we report a detailed study on a series of dynamic combinatorial frameworks (DCFs) containing PEGylated squalene, poly-(ethyleneglycol)-bis(3-aminopropyl) of different lengths, and branched low molecular weight polyethylenimine components, reversibly connected in hyperbranched structures, as efficient dynamic non-viral vectors. The obtained frameworks were capable of forming distinct supramolecular amphiphilic architectures, shown by transmission electron microscopy (TEM) and dynamic light scattering (DLS), with sizes and stability depending on the length of PEG units. The interaction of PEGylated DCFs with nucleic acids was investigated by agarose gel retardation assay and atomic force microscopy (AFM), while their transfection efficiency (using pCS2+MT-Luc DNA as a reporter gene) and cytotoxicity were evaluated in HeLa cells. In addition, the data on the influence of the poly-(ethyleneglycol)-bis(3-aminopropyl) length in composition of designed frameworks over transfection efficiency and tolerance in human cells were analyzed and compared. View Full-Text
Keywords: polyethyleneglycol; polyethyleneimine; pegylated squalene; dynamic combinatorial chemistry; pDNA condensation; non-viral vectors; gene therapy; DNA transfection polyethyleneglycol; polyethyleneimine; pegylated squalene; dynamic combinatorial chemistry; pDNA condensation; non-viral vectors; gene therapy; DNA transfection
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MDPI and ACS Style

Clima, L.; Craciun, B.F.; Gavril, G.; Pinteala, M. Tunable Composition of Dynamic Non-Viral Vectors over the DNA Polyplex Formation and Nucleic Acid Transfection. Polymers 2019, 11, 1313. https://doi.org/10.3390/polym11081313

AMA Style

Clima L, Craciun BF, Gavril G, Pinteala M. Tunable Composition of Dynamic Non-Viral Vectors over the DNA Polyplex Formation and Nucleic Acid Transfection. Polymers. 2019; 11(8):1313. https://doi.org/10.3390/polym11081313

Chicago/Turabian Style

Clima, Lilia; Craciun, Bogdan F.; Gavril, Gabriela; Pinteala, Mariana. 2019. "Tunable Composition of Dynamic Non-Viral Vectors over the DNA Polyplex Formation and Nucleic Acid Transfection" Polymers 11, no. 8: 1313. https://doi.org/10.3390/polym11081313

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