Next Article in Journal
Writing Behavior of Phospholipids in Polymer Pen Lithography (PPL) for Bioactive Micropatterns
Previous Article in Journal
Molecularly Imprinted Polymer Materials as Selective Recognition Sorbents for Explosives: A Review
Previous Article in Special Issue
Characterization and Optimization of PLA Stereocomplexed Hydrogels for Local Gene Delivery Systems
Open AccessArticle

Combining Inulin Multifunctional Polycation and Magnetic Nanoparticles: Redox-Responsive siRNA-Loaded Systems for Magnetofection

Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università di Palermo, Via Archirafi 32, 90123 Palermo, Italy
*
Author to whom correspondence should be addressed.
Polymers 2019, 11(5), 889; https://doi.org/10.3390/polym11050889
Received: 12 April 2019 / Revised: 3 May 2019 / Accepted: 9 May 2019 / Published: 15 May 2019
(This article belongs to the Special Issue Polymer-Based Systems for Controlled Release and Targeting of Drugs)
Superparamagnetic Iron Oxide Nanoparticles (SPIONs) are recognized as one of the most promising agents for theranostic applications. Among methods designed for siRNA delivery, magnetofection, that is, nucleic acid cell uptake under the influence of a magnetic field acting on magnetic nucleic acid vectors, is emerging as a unique approach to combining advantages such as strong improvement of the kinetics of the delivery process and the possibility of localizing nucleic acid delivery to an area where the magnetic field is applied. This paper reports on the preparation of siRNA loaded magnetoplexes—named [email protected]@SPIONs/siRNA—by controlled crosslinking, in the presence of SPIONs, of the polycation INU-C-DETA, synthesized starting from the polysaccharide inulin by grafting diethylenetriamine and cystamine molecules. The obtained [email protected]@SPIONs/siRNA have suitable chemical-physical characteristics to be employed for iv administration and are also able to release siRNA in a redox-triggered manner thanks to intracellular glutathione (GSH) mediated reduction of disulphide bridges formed during the crosslinking process. Moreover, [email protected]@SPIONs/siRNA are able to produce magnetic targeting in vitro on breast cancer cells, without appreciable cyto- and hemo-toxic effects, in a wide range of concentrations. Finally, protein binding to nanoparticles revealed that obtained systems are potentially longer circulating and applicable as a smart multifunctional agents for cancer therapy. View Full-Text
Keywords: inulin; cystamine; DETA; siRNA; SPIONs inulin; cystamine; DETA; siRNA; SPIONs
Show Figures

Graphical abstract

MDPI and ACS Style

Sardo, C.; Craparo, E.F.; Porsio, B.; Giammona, G.; Cavallaro, G. Combining Inulin Multifunctional Polycation and Magnetic Nanoparticles: Redox-Responsive siRNA-Loaded Systems for Magnetofection. Polymers 2019, 11, 889.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop