Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane

Qi, Meng; Zhao, Kongyin; Bao, Qiwen; Pan, Peng; Zhao, Yuwei; Yang, Zhengchun; Wang, Huiquan; Wei, Junfu

doi:10.3390/polym11040622

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Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane

by

Meng Qi

^1,2,

Kongyin Zhao

^1,2,*

,

Qiwen Bao

³,

Peng Pan

³,

Yuwei Zhao

²,

Zhengchun Yang

^3,*,

Huiquan Wang

² and

Junfu Wei

¹

State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin 300387, China

²

School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China

³

Tianjin Key Laboratory of Membrane Electronic & Communication Devices, Tianjin University of Technology, Tianjin 300384, China

^*

Authors to whom correspondence should be addressed.

Polymers 2019, 11(4), 622; https://doi.org/10.3390/polym11040622

Submission received: 19 January 2019 / Revised: 17 March 2019 / Accepted: 26 March 2019 / Published: 4 April 2019

(This article belongs to the Special Issue Biomimicry through Molecular Imprinting: From Polymer Design to Devices)

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Abstract

In this paper, bovine serum albumin (BSA)-imprinted calcium alginate (CaAlg) hydrogel membrane was prepared using BSA as a template, sodium alginate (NaAlg) as a functional monomer, and CaCl₂ as a cross-linker. The thickness of the CaAlg membrane was controlled by a glass rod enlaced with brass wires (the diameter was 0.1, 0.2, 0.3, 0.4, and 0.5 mm). The swelling properties of the CaAlg membranes prepared with different contents of NaAlg were researched. Circular dichroism indicated that the conformation of BSA did not change during the preparing and eluting process. The thinner the CaAlg hydrogel membrane was, the larger the adsorption capacity and the higher the imprinting efficiency of the CaAlg. The maximum adsorption capacity of molecularly imprinted polymer (MIP) and non-imprinted CaAlg hydrogel membrane (NIP) was 38.6 mg·g⁻¹ and 9.2 mg·g⁻¹, respectively, with an imprinting efficiency of 4.2. The MIP was loaded on the electrode to monitor the selective adsorption of BSA by voltammetry curve.

Keywords: calcium alginate; bovine serum albumin; molecular imprinting; hydrogel membrane; cyclic voltammetry

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MDPI and ACS Style

Qi, M.; Zhao, K.; Bao, Q.; Pan, P.; Zhao, Y.; Yang, Z.; Wang, H.; Wei, J. Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane. Polymers 2019, 11, 622. https://doi.org/10.3390/polym11040622

AMA Style

Qi M, Zhao K, Bao Q, Pan P, Zhao Y, Yang Z, Wang H, Wei J. Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane. Polymers. 2019; 11(4):622. https://doi.org/10.3390/polym11040622

Chicago/Turabian Style

Qi, Meng, Kongyin Zhao, Qiwen Bao, Peng Pan, Yuwei Zhao, Zhengchun Yang, Huiquan Wang, and Junfu Wei. 2019. "Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane" Polymers 11, no. 4: 622. https://doi.org/10.3390/polym11040622

APA Style

Qi, M., Zhao, K., Bao, Q., Pan, P., Zhao, Y., Yang, Z., Wang, H., & Wei, J. (2019). Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane. Polymers, 11(4), 622. https://doi.org/10.3390/polym11040622

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Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane

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