Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Interferon Alpha (IFNα)-Loaded Nanoparticles
2.3. Characterization of the Nanoparticles
2.3.1. Hydrodynamic Diameter, Polydispersity Index and Zeta-Potential
2.3.2. Nanoparticle-Tracking Analysis
2.3.3. Stability of Nanoparticles as a Function of pH
2.3.4. Drug-Encapsulation Efficiency
2.4. Cell Studies In Vitro
2.4.1. Cells
2.4.2. Cell Compatibility and Permeability Assays
2.4.3. Cell Uptake
2.5. Oral Pharmacokinetics
2.6. Statistical Analysis
3. Results and Discussions
3.1. Synthesis and Characterization of PEGylated Chitosan
3.2. Preparation and Characterization of the Nanoparticles
3.3. Cell Compatibility, Permeability and Uptake
3.4. Oral Pharmacokinetics
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameter | CT-NPs | PEG.CT-NPs |
---|---|---|
Dh (nm) | 47 | 93 |
PDI | 0.47 | 0.32 |
Concentration (particles/mL) | 3 × 1011 ± 6 × 107 | 3 × 1011 ± 3 × 1010 |
Parameter | Value |
---|---|
tmax1 (h) | 0.5 |
tmax2 (h) | 1.5 |
Cmax1 (pg.mL−1) | 48.4 ± 22.5 |
Cmax2 (pg.mL−1) | 27.6 ± 31.4 |
t1/2 (h) | 0.07 ± 0.02 |
AUC0-∞ (pg*h/mL) | 56.92 |
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Imperiale, J.C.; Schlachet, I.; Lewicki, M.; Sosnik, A.; Biglione, M.M. Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha. Polymers 2019, 11, 1862. https://doi.org/10.3390/polym11111862
Imperiale JC, Schlachet I, Lewicki M, Sosnik A, Biglione MM. Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha. Polymers. 2019; 11(11):1862. https://doi.org/10.3390/polym11111862
Chicago/Turabian StyleImperiale, Julieta C., Inbar Schlachet, Marianela Lewicki, Alejandro Sosnik, and Mirna M. Biglione. 2019. "Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha" Polymers 11, no. 11: 1862. https://doi.org/10.3390/polym11111862