Synthesis, Characterization, and the Antioxidant Activity of Carboxymethyl Chitosan Derivatives Containing Thiourea Salts
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Analytical Methods
2.2.1. Fourier Transform Infrared (FT-IR) Spectroscopy
2.2.2. Nuclear Magnetic Resonance (NMR) Spectroscopy
2.2.3. Elemental Analyses
2.2.4. Thermogravimetric Analysis (TGA)
2.3. Synthesis of Chitosan Derivatives
2.3.1. Synthesis of N, O-carboxymethyl Chitosan (N, O-CMCS)
2.3.2. Synthesis of 4MTCMCS, TCMCS, 4,4DCMCS, and 4ACMCS
2.4. Antioxidant Activity Assay
2.4.1. DPPH Radicals’ Scavenging Ability Assay
2.4.2. Superoxide-Radical Scavenging Activity Assay
2.4.3. Hydroxyl-Radical Scavenging Activity Assay
2.5. Cytotoxicity Assay
3. Results and Discussion
3.1. Structure of CS, N, O-CMCS, and Carboxymethyl Chitosan Containing Thiourea Salts
3.1.1. Elemental Analyses
3.1.2. Infrared Spectroscopy
3.1.3. NMR Spectra
3.1.4. Thermogravimetric and Derivative Thermogravimetric Analysis (TGA/DTG)
3.2. Antioxidant Activity
3.2.1. Scavenging Ability of DPPH Radical
3.2.2. Scavenging Ability of the Superoxide Radical
3.2.3. Scavenging Ability of Hydroxyl Radical
3.3. Cytotoxicity Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Compounds | Yields (%) | Elemental Analyses (%) | Degrees of Substitution (%) | ||||
---|---|---|---|---|---|---|---|
C | N | S | C/N | C/S | |||
CS | - | 40.86 | 7.28 | - | 5.62 | - | 73.5 |
N, O-CMCS | 75.85 | 35.24 | 3.96 | - | 8.90 | 96.27 | |
4TCMCS | 42.56 | 35.50 | 5.39 | 1.50 | 6.59 | 23.72 | 8.47 |
TCMCS | 27.71 | 36.25 | 5.71 | 1.57 | 6.35 | 23.14 | 8.55 |
4,4DCMCS | 26.52 | 35.47 | 4.36 | 1.18 | 8.14 | 30.06 | 6.89 |
4ACMCS | 20.41 | 35.06 | 5.14 | 1.34 | 6.83 | 26.24 | 7.64 |
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Sun, X.; Zhang, J.; Chen, Y.; Mi, Y.; Tan, W.; Li, Q.; Dong, F.; Guo, Z. Synthesis, Characterization, and the Antioxidant Activity of Carboxymethyl Chitosan Derivatives Containing Thiourea Salts. Polymers 2019, 11, 1810. https://doi.org/10.3390/polym11111810
Sun X, Zhang J, Chen Y, Mi Y, Tan W, Li Q, Dong F, Guo Z. Synthesis, Characterization, and the Antioxidant Activity of Carboxymethyl Chitosan Derivatives Containing Thiourea Salts. Polymers. 2019; 11(11):1810. https://doi.org/10.3390/polym11111810
Chicago/Turabian StyleSun, Xueqi, Jingjing Zhang, Yuan Chen, Yingqi Mi, Wenqiang Tan, Qing Li, Fang Dong, and Zhanyong Guo. 2019. "Synthesis, Characterization, and the Antioxidant Activity of Carboxymethyl Chitosan Derivatives Containing Thiourea Salts" Polymers 11, no. 11: 1810. https://doi.org/10.3390/polym11111810