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Article

Preparation of Nafion/Polycation Layer-by-Layer Films for Adsorption and Release of Insulin

1
School of Pharmaceutical Sciences, Ohu University, 31-1 Misumido, Tomita-machi, Koriyama, Fukushima 963-8611, Japan
2
Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan
*
Author to whom correspondence should be addressed.
Polymers 2018, 10(8), 812; https://doi.org/10.3390/polym10080812
Received: 22 June 2018 / Revised: 20 July 2018 / Accepted: 23 July 2018 / Published: 24 July 2018
Thin films were prepared using layer-by-layer (LbL) deposition of Nafion (NAF) and polycations such as poly(allylamine hydrochloride) (PAH), poly(ethyleneimine) (PEI), and poly(diallydimethylammonium chloride) (PDDA). Insulin was then adsorbed on the NAF-polycation LbL films by immersion in an insulin solution. The NAF-polycation LbL films were characterized using a quartz crystal microbalance and an atomic force microscope. The release of insulin from the LbL films was characterized using UV-visible adsorption spectroscopy and fluorescence emission spectroscopy. The greatest amount of insulin was adsorbed on the NAF-PAH LbL film. The amount of insulin adsorbed on the (NAF/PAH)5NAF LbL films by immersion in a 1 mg mL−1 insulin solution at pH 7.4 was 61.8 µg cm−2. The amount of insulin released from the LbL films was higher when immersed in insulin solutions at pH 2.0 and pH 9.0 than at pH 7.4. Therefore, NAF-polycations could be employed as insulin delivery LbL films under mild conditions and as an insulin release control system according to pH change. View Full-Text
Keywords: layer-by-layer film; controlled release; insulin; Nafion; drug delivery layer-by-layer film; controlled release; insulin; Nafion; drug delivery
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MDPI and ACS Style

Yoshida, K.; Sato, K.; Ono, T.; Dairaku, T.; Kashiwagi, Y. Preparation of Nafion/Polycation Layer-by-Layer Films for Adsorption and Release of Insulin. Polymers 2018, 10, 812. https://doi.org/10.3390/polym10080812

AMA Style

Yoshida K, Sato K, Ono T, Dairaku T, Kashiwagi Y. Preparation of Nafion/Polycation Layer-by-Layer Films for Adsorption and Release of Insulin. Polymers. 2018; 10(8):812. https://doi.org/10.3390/polym10080812

Chicago/Turabian Style

Yoshida, Kentaro, Katsuhiko Sato, Tetsuya Ono, Takenori Dairaku, and Yoshitomo Kashiwagi. 2018. "Preparation of Nafion/Polycation Layer-by-Layer Films for Adsorption and Release of Insulin" Polymers 10, no. 8: 812. https://doi.org/10.3390/polym10080812

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