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Polymers 2018, 10(7), 738; https://doi.org/10.3390/polym10070738

Human Engineered Cartilage and Decellularized Matrix as an Alternative to Animal Osteoarthritis Model

1
Tissue Therapy Unit, ASST Niguarda Hospital, Piazza Ospedale Maggiore 3, 20162 Milan, Italy
2
Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy
3
PharmaExceed S.r.l., 27100 Pavia, Italy
4
Section of Histopathology, Department of Surgical Sciences and Integrated Diagnostics (DISC), IRCCS San Martino IST Hospital, University of Genoa, Largo R. Benzi 8, 16121 Genoa, Italy
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 1 June 2018 / Revised: 28 June 2018 / Accepted: 3 July 2018 / Published: 4 July 2018
(This article belongs to the Special Issue Polymer Scaffolds for Biomedical Application)
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Abstract

(1) Objective: to obtain a reproducible, robust, well-defined, and cost-affordable in vitro model of human cartilage degeneration, suitable for drug screening; (2) Methods: we proposed 3D models of engineered cartilage, considering two human chondrocyte sources (articular/nasal) and five culture methods (pellet, alginate beads, silk/alginate microcarriers, and decellularized cartilage). Engineered cartilages were treated with pro-inflammatory cytokine IL-1β to promote cartilage degradation; (3) Results: articular chondrocytes have been rejected since they exhibit low cellular doubling with respect to nasal cells, with longer culture time for cell expansion; furthermore, pellet and alginate bead cultures lead to insufficient cartilage matrix production. Decellularized cartilage resulted as good support for degeneration model, but long culture time and high cell amount are required to obtain the adequate scaffold colonization. Here, we proposed, for the first time, the combined use of decellularized cartilage, as aggrecanase substrate, with pellet, alginate beads, or silk/alginate microcarriers, as polymeric scaffolds for chondrocyte cultures. This approach enables the development of suitable models of cartilaginous pathology. The results obtained after cryopreservation also demonstrated that beads and microcarriers are able to preserve chondrocyte functionality and metabolic activity; (4) Conclusions: alginate and silk/alginate-based scaffolds can be easily produced and cryopreserved to obtain a cost-affordable and ready-to-use polymer-based product for the subsequent screening of anti-inflammatory drugs for cartilage diseases. View Full-Text
Keywords: human chondrocytes; osteoarthritis; alginate; silk fibroin; beads; microcarrier; pellet; decellularized cartilage matrix human chondrocytes; osteoarthritis; alginate; silk fibroin; beads; microcarrier; pellet; decellularized cartilage matrix
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Galuzzi, M.; Perteghella, S.; Antonioli, B.; Tosca, M.C.; Bari, E.; Tripodo, G.; Sorrenti, M.; Catenacci, L.; Mastracci, L.; Grillo, F.; Marazzi, M.; Torre, M.L. Human Engineered Cartilage and Decellularized Matrix as an Alternative to Animal Osteoarthritis Model. Polymers 2018, 10, 738.

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