Polymers 2018, 10(2), 174; https://doi.org/10.3390/polym10020174
Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration
Catalina Biglione 1,2,†, Julian Bergueiro 2,†
, Mazdak Asadian-Birjand 2, Christoph Weise 2, Vrushali Khobragade 3,4, Govind Chate 5, Manoj Dongare 3,4,5, Jayant Khandare 3,4,5, Miriam C. Strumia 1 and Marcelo Calderón 2,*
, Mazdak Asadian-Birjand 2, Christoph Weise 2, Vrushali Khobragade 3,4, Govind Chate 5, Manoj Dongare 3,4,5, Jayant Khandare 3,4,5, Miriam C. Strumia 1 and Marcelo Calderón 2,*
1
LAMAP Laboratorio de Materiales Poliméricos, IPQA-CONICET, Departamento de Química, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, X5000HUA Córdoba, Argentina
2
Freie Universität Berlin, Institute of Chemistry and Biochemistry, Takustr. 3, 14195 Berlin, Germany
3
Actorius Innovations and Research, B 411, GO Square, Wakad Road, 411057 Pune, India
4
Surgical Oncologist, Manik Hospital and Research Center, Aurangabad 431001, India
5
MAEER’s Maharashtra Institute of Pharmacy, Kothrud, Pune 411038, Maharashtra, India
†
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 24 November 2017 / Revised: 19 January 2018 / Accepted: 6 February 2018 / Published: 11 February 2018
(This article belongs to the Special Issue Advance of Polymers Applied to Biomedical Applications: Biointerface)
Abstract
Magnetic nanogels (MNGs) are designed to have all the required features for their use as highly efficient trapping materials in the challenging task of selectively capturing circulating tumor cells (CTCs) from the bloodstream. Advantageously, the discrimination of CTCs from hematological cells, which is a key factor in the capturing process, can be optimized by finely tuning the polymers used to link the targeting moiety to the MNG. We describe herein the relationship between the capturing efficiency of CTCs with overexpressed transferrin receptors and the different strategies on the polymer used as linker to decorate these MNGs with transferrin (Tf). Heterobifunctional polyethylene glycol (PEG) linkers with different molecular weights were coupled to Tf in different ratios. Optimal values over 80% CTC capture efficiency were obtained when 3 PEG linkers with a length of 8 ethylene glycol (EG) units were used, which reveals the important role of the linker in the design of a CTC-sorting system. View Full-TextKeywords:
circulating tumor cells; magnetic nanogel; cell sorting; transferrin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Biglione, C.; Bergueiro, J.; Asadian-Birjand, M.; Weise, C.; Khobragade, V.; Chate, G.; Dongare, M.; Khandare, J.; Strumia, M.C.; Calderón, M. Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration. Polymers 2018, 10, 174.
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