Next Article in Journal
Site-Specific DBCO Modification of DEC205 Antibody for Polymer Conjugation
Previous Article in Journal
PLA Melt Stabilization by High-Surface-Area Graphite and Carbon Black
Previous Article in Special Issue
Advanced Control over Cell-Material Interfaces
 
Search for Articles:
Title / Keyword
Author / Affiliation
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
Journals
Polymers
Volume 10
Issue 2
10.3390/polym10020140
polymers-logo
Submit to this Journal Review for this Journal Edit a Special Issue
► Article Menu

Article Menu

Printed Edition

A printed edition of this Special Issue is available at MDPI Books....
share announcement question_answer thumb_up
...
textsms
...
Article

Surface Hydrophilicity of Poly(l-Lactide) Acid Polymer Film Changes the Human Adult Adipose Stem Cell Architecture

by
Chiara Argentati
1,†,
Francesco Morena
1,†,
Pia Montanucci
2,
Marco Rallini
3,
Giuseppe Basta
2,
Nicolino Calabrese
4,
Riccardo Calafiore
2,
Marino Cordellini
5,
Carla Emiliani
1,
Ilaria Armentano
6 and
Sabata Martino
1,*
1
Department of Chemistry, Biology and Biotechnologies, Biochemistry and Molecular Biology Unit, University of Perugia, Via del Giochetto, 06126 Perugia, Italy
2
Section of Cardiovascular, Endocrine and Metabolic Clinical Physiology, Laboratory for Endocrine Cell Transplants and Biohybrid Organs, Department of Medicine, University of Perugia, 06126 Perugia, Italy
3
Civil and Environmental Engineering Department, UdR INSTM, University of Perugia, 05100 Terni, Italy
4
Private Dental Practice, 06134 Perugia, Italy
5
Plastic and Reconstructive Surgery Unit, 06024 ASL 1 Umbria, Italy
6
Department of Ecological and Biological Sciences, Tuscia University, 01100 Viterbo, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Polymers 2018, 10(2), 140; https://doi.org/10.3390/polym10020140
Received: 24 November 2017 / Revised: 23 January 2018 / Accepted: 31 January 2018 / Published: 1 February 2018
(This article belongs to the Special Issue Advance of Polymers Applied to Biomedical Applications: Cell Scaffolds)
View Full-Text Download PDF
Browse Figures

Abstract

Current knowledge indicates that the molecular cross-talk between stem cells and biomaterials guides the stem cells’ fate within a tissue engineering system. In this work, we have explored the effects of the interaction between the poly(l-lactide) acid (PLLA) polymer film and human adult adipose stem cells (hASCs), focusing on the events correlating the materials’ surface characteristics and the cells’ plasma membrane. hASCs were seeded on films of pristine PLLA polymer and on a PLLA surface modified by the radiofrequency plasma method under oxygen flow (PLLA+O2). Comparative experiments were performed using human bone-marrow mesenchymal stem cells (hBM-MSCs) and human umbilical matrix stem cells (hUCMSCs). After treatment with oxygen-plasma, the surface of PLLA films became hydrophilic, whereas the bulk properties were not affected. hASCs cultured on pristine PLLA polymer films acquired a spheroid conformation. On the contrary, hASCs seeded on PLLA+O2 film surface maintained the fibroblast-like morphology typically observed on tissue culture polystyrene. This suggests that the surface hydrophilicity is involved in the acquisition of the spheroid conformation. Noteworthy, the oxygen treatment had no effects on hBM-MSC and hUCMSC cultures and both stem cells maintained the same shape observed on PLLA films. This different behavior suggests that the biomaterial-interaction is stem cell specific. View Full-Text
Keywords: cytoskeleton architecture; stem cell fate; regenerative medicine cytoskeleton architecture; stem cell fate; regenerative medicine
►▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
SciFeed

Share and Cite

MDPI and ACS Style

Argentati, C.; Morena, F.; Montanucci, P.; Rallini, M.; Basta, G.; Calabrese, N.; Calafiore, R.; Cordellini, M.; Emiliani, C.; Armentano, I.; Martino, S. Surface Hydrophilicity of Poly(l-Lactide) Acid Polymer Film Changes the Human Adult Adipose Stem Cell Architecture. Polymers 2018, 10, 140. https://doi.org/10.3390/polym10020140

AMA Style

Argentati C, Morena F, Montanucci P, Rallini M, Basta G, Calabrese N, Calafiore R, Cordellini M, Emiliani C, Armentano I, Martino S. Surface Hydrophilicity of Poly(l-Lactide) Acid Polymer Film Changes the Human Adult Adipose Stem Cell Architecture. Polymers. 2018; 10(2):140. https://doi.org/10.3390/polym10020140

Chicago/Turabian Style

Argentati, Chiara, Francesco Morena, Pia Montanucci, Marco Rallini, Giuseppe Basta, Nicolino Calabrese, Riccardo Calafiore, Marino Cordellini, Carla Emiliani, Ilaria Armentano, and Sabata Martino. 2018. "Surface Hydrophilicity of Poly(l-Lactide) Acid Polymer Film Changes the Human Adult Adipose Stem Cell Architecture" Polymers 10, no. 2: 140. https://doi.org/10.3390/polym10020140

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Map by Country/Region

1
Back to TopTop