Acrylated Chitosan Nanoparticles with Enhanced Mucoadhesion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of Acrylated Chitosan
2.3. Synthesis of Fluorescein Isocyanate (FITC)-Labeled Polymers
2.4. Polymer Characterization
2.4.1. Nuclear Magnetic Resonance (NMR)
2.4.2. Fluorescamine Test for Quantitative Determination of Acrylate Groups
2.5. Fabrication of Nanoparticles
2.6. Nanoparticle Characterization
2.6.1. Size and Zeta Potential
2.6.2. Yield
2.7. Mucoadhesion Studies
2.7.1. Nanoparticle Retention Studies
2.7.2. Nanoparticle-Mucin Aggregation
2.8. Statistical Data Analysis
3. Results and Discussion
3.1. Acrylation of Chitosan
3.2. Fabrication of Nanoparticles
3.3. Stability of the Nanoparticles
3.4. Nanoparticle Yield
3.5. Mucoadhesion Characterization
3.5.1. Nanoparticle Retention on Mucosal Surfaces
3.5.2. Nanoparticle-Mucin Aggregation
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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CS Concentration (mg/mL) | TPP Concentration (mg/mL) | Size by Intensity (nm) |
---|---|---|
2 | 1 | 354.6 ± 4.5 |
2 | 0.5 | 317.0 ± 6.6 |
2 | 0.25 | 249.0 ± 3.4 |
1 | 0.5 | 228.6 ± 0.8 |
1 | 0.25 | 209.4 ± 3.0 |
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Eliyahu, S.; Aharon, A.; Bianco-Peled, H. Acrylated Chitosan Nanoparticles with Enhanced Mucoadhesion. Polymers 2018, 10, 106. https://doi.org/10.3390/polym10020106
Eliyahu S, Aharon A, Bianco-Peled H. Acrylated Chitosan Nanoparticles with Enhanced Mucoadhesion. Polymers. 2018; 10(2):106. https://doi.org/10.3390/polym10020106
Chicago/Turabian StyleEliyahu, Shaked, Anat Aharon, and Havazelet Bianco-Peled. 2018. "Acrylated Chitosan Nanoparticles with Enhanced Mucoadhesion" Polymers 10, no. 2: 106. https://doi.org/10.3390/polym10020106