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Bacterial Effect on the Crystallization of Mineral Phases in a Solution Simulating Human Urine

1
Crystallography Department, Institute of Earth Sciences, St. Petersburg State University, St. Petersburg 199034, Russia
2
Grebenshchikov Institute of Silicate Chemistry of the Russian Academy of Sciences, St. Petersburg 199034, Russia
3
Microbiology Department, S.M. Kirov Military Medical Academy, St. Petersburg 194044, Russia
*
Author to whom correspondence should be addressed.
Crystals 2019, 9(5), 259; https://doi.org/10.3390/cryst9050259
Received: 24 April 2019 / Revised: 11 May 2019 / Accepted: 16 May 2019 / Published: 18 May 2019
(This article belongs to the Special Issue Mineralogical Crystallography)
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Abstract

The effect of bacteria that present in the human urine (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus) was studied under the conditions of biomimetic synthesis. It was shown that the addition of bacteria significantly affects both the phase composition of the synthesized material and the position of crystallization boundaries of the resulting phosphate phases, which can shift toward more acidic (struvite, apatite) or toward more alkaline (brushite) conditions. Under conditions of oxalate mineralization, bacteria accelerate the nucleation of calcium oxalates by almost two times and also increase the amount of oxalate precipitates along with phosphates and stabilize the calcium oxalate dihydrate (weddellite). The multidirectional changes in the pH values of the solutions, which are the result of the interaction of all system components and the crystallization process, were analyzed. The obtained results are the scientific basis for understanding the mechanisms of bacterial involvement in stone formation within the human body and the creation of biotechnological methods that inhibit this process. View Full-Text
Keywords: pathogen crystallization; biomimetic synthesis; renal stone; calcium oxalate; apatite; brushite; struvite; octocalcium phosphate; whitlockite; Escherichia coli; Klebsiella pneumoniae; Pseudomonas aeruginosa; Staphylococcus aureus pathogen crystallization; biomimetic synthesis; renal stone; calcium oxalate; apatite; brushite; struvite; octocalcium phosphate; whitlockite; Escherichia coli; Klebsiella pneumoniae; Pseudomonas aeruginosa; Staphylococcus aureus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Izatulina, A.R.; Nikolaev, A.M.; Kuz’mina, M.A.; Frank-Kamenetskaya, O.V.; Malyshev, V.V. Bacterial Effect on the Crystallization of Mineral Phases in a Solution Simulating Human Urine. Crystals 2019, 9, 259.

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