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Crystals 2018, 8(11), 422; https://doi.org/10.3390/cryst8110422

Peculiarities of Protein Crystal Nucleation and Growth

Rostislaw Kaischew Institute of Physical Chemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
Received: 18 October 2018 / Revised: 31 October 2018 / Accepted: 5 November 2018 / Published: 8 November 2018
(This article belongs to the Special Issue Biological Crystallization)
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Abstract

This paper reviews investigations on protein crystallization. It aims to present a comprehensive rather than complete account of recent studies and efforts to elucidate the most intimate mechanisms of protein crystal nucleation. It is emphasized that both physical and biochemical factors are at play during this process. Recently-discovered molecular scale pathways for protein crystal nucleation are considered first. The bond selection during protein crystal lattice formation, which is a typical biochemically-conditioned peculiarity of the crystallization process, is revisited. Novel approaches allow us to quantitatively describe some protein crystallization cases. Additional light is shed on the protein crystal nucleation in pores and crevices by employing the so-called EBDE method (equilibration between crystal bond and destructive energies). Also, protein crystal nucleation in solution flow is considered. View Full-Text
Keywords: protein crystallization; biochemical aspects of the protein crystal nucleation; classical and two-step crystal nucleation mechanisms; bond selection during protein crystallization; equilibration between crystal bond and destructive energies; protein crystal nucleation in pores; crystallization in solution flow protein crystallization; biochemical aspects of the protein crystal nucleation; classical and two-step crystal nucleation mechanisms; bond selection during protein crystallization; equilibration between crystal bond and destructive energies; protein crystal nucleation in pores; crystallization in solution flow
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Nanev, C.N. Peculiarities of Protein Crystal Nucleation and Growth. Crystals 2018, 8, 422.

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