Next Article in Journal
Double Palindrome Water Chain in Cu(II) Theophylline Complex. Synthesis, Characterization, Biological Activity of Cu(II), Zn(II) Complexes with Theophylline
Previous Article in Journal
Scintillator Crystals: Structure, Characterization and Models for Better Performances
Previous Article in Special Issue
Monitoring the Production of High Diffraction-Quality Crystals of Two Enzymes in Real Time Using In Situ Dynamic Light Scattering
Open AccessArticle

Tools to Ease the Choice and Design of Protein Crystallisation Experiments

Biomedical Manufacturing, CSIRO Parkville, 343 Royal Parade, Parkville Vic 3052, Australia
Scientific Computing, CSIRO Clayton, Research Way, Clayton Vic 3168, Australia
Author to whom correspondence should be addressed.
Current address: Department of Engineering, Monash University, Wellington Rd, Clayton ViC 3800, Australia.
Current address: Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany.
Crystals 2020, 10(2), 95;
Received: 16 January 2020 / Revised: 30 January 2020 / Accepted: 31 January 2020 / Published: 7 February 2020
(This article belongs to the Special Issue Novel Strategies for Improved Protein Crystallization)
The process of macromolecular crystallisation almost always begins by setting up crystallisation trials using commercial or other premade screens, followed by cycles of optimisation where the crystallisation cocktails are focused towards a particular small region of chemical space. The screening process is relatively straightforward, but still requires an understanding of the plethora of commercially available screens. Optimisation is complicated by requiring both the design and preparation of the appropriate secondary screens. Software has been developed in the C3 lab to aid the process of choosing initial screens, to analyse the results of the initial trials, and to design and describe how to prepare optimisation screens. View Full-Text
Keywords: crystallization cocktail; crystallization screen; crystallization optimisation; similarity crystallization cocktail; crystallization screen; crystallization optimisation; similarity
Show Figures

Figure 1

MDPI and ACS Style

Rosa, N.; Ristic, M.; Thorburn, L.; Abrahams, G.J.; Marshall, B.; Watkins, C.J.; Kruger, A.; Khassapov, A.; Newman, J. Tools to Ease the Choice and Design of Protein Crystallisation Experiments. Crystals 2020, 10, 95.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop