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Open AccessArticle

Tools to Ease the Choice and Design of Protein Crystallisation Experiments

1
Biomedical Manufacturing, CSIRO Parkville, 343 Royal Parade, Parkville Vic 3052, Australia
2
Scientific Computing, CSIRO Clayton, Research Way, Clayton Vic 3168, Australia
*
Author to whom correspondence should be addressed.
Current address: Department of Engineering, Monash University, Wellington Rd, Clayton ViC 3800, Australia.
Current address: Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany.
Crystals 2020, 10(2), 95; https://doi.org/10.3390/cryst10020095
Received: 16 January 2020 / Revised: 30 January 2020 / Accepted: 31 January 2020 / Published: 7 February 2020
(This article belongs to the Special Issue Novel Strategies for Improved Protein Crystallization)
The process of macromolecular crystallisation almost always begins by setting up crystallisation trials using commercial or other premade screens, followed by cycles of optimisation where the crystallisation cocktails are focused towards a particular small region of chemical space. The screening process is relatively straightforward, but still requires an understanding of the plethora of commercially available screens. Optimisation is complicated by requiring both the design and preparation of the appropriate secondary screens. Software has been developed in the C3 lab to aid the process of choosing initial screens, to analyse the results of the initial trials, and to design and describe how to prepare optimisation screens. View Full-Text
Keywords: crystallization cocktail; crystallization screen; crystallization optimisation; similarity crystallization cocktail; crystallization screen; crystallization optimisation; similarity
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Rosa, N.; Ristic, M.; Thorburn, L.; Abrahams, G.J.; Marshall, B.; Watkins, C.J.; Kruger, A.; Khassapov, A.; Newman, J. Tools to Ease the Choice and Design of Protein Crystallisation Experiments. Crystals 2020, 10, 95.

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