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Article

A Highly Water-Soluble Fluorescent and Colorimetric pH Probe

by
Rosita Diana
1,
Ugo Caruso
2,*,
Angela Tuzi
2 and
Barbara Panunzi
1
1
Department of Agriculture, University of Napoli Federico II, 80055 Portici NA, Italy
2
Department of Chemical Sciences, University of Napoli Federico II, 80126 Napoli, Italy
*
Author to whom correspondence should be addressed.
Crystals 2020, 10(2), 83; https://doi.org/10.3390/cryst10020083
Submission received: 20 January 2020 / Revised: 30 January 2020 / Accepted: 1 February 2020 / Published: 3 February 2020
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Abstract

:
A new 5-(4-((2-(benzothiazole-2-carbonyl)hydrazono)methyl)-3-hydroxyphenoxy)-N,N,N-trimethylpentan-1-aminium bromide (BTABr) fluorescent and colorimetric pH probe was easily synthesized by the condensation reaction of benzothiazole-2-carbohydrazide with 5-(4-formyl-3-hydroxyphenoxy)-N,N,N-trimethylpentan-1-aminium bromide. The benzothiazole moiety provided the emissive part of the molecule and the charged trimethyl amino group guaranteed outstanding solubility in water, for an organic molecule. pH titration experiments indicated that the probe is useful for monitoring acidic and alkaline solutions, turning reversibly in color/fluorescence just at a neutral pH value. Naked-eye colorimetric response was observed both in solution and in the solid state. In addition, the probe showed high stability and selectivity and large Stokes shifts. Because of these features, BTABr can potentially work as an on-off real-time pH sensor for intracellular pH imaging. The crystal structure of BTABr examined by single-crystal analysis showed a planar geometry of the molecule and confirmed the presence of a molecular stacking between molecules joined in a complex tridimensional hydrogen bonding pattern.

Graphical Abstract

1. Introduction

The design of pH-responsive molecules is a remarkable field of research, finding applications in environmental, biochemical, electrochemical, and analytical technologies [1,2]. As a key parameter for cell survival, its determination is crucial in living systems. Optical pH sensors are currently considered the cutting edge in the field of chemosensors due to the ease of use, low-cost process, high sensitivity and selectivity, and fast response [3,4,5,6].
Many optical pH sensors are both colorimetric and fluorometric pH probes. Typical fluorescence pH sensors are based on photoinduced electron transfer (PET), intramolecular charge transfer (ICT), and electron transfer (ET) effect. Organic molecules with a photon interaction site undergo electronic transfer effects turning in different tautomeric forms in a dependent manner from pH. Among them, Schiff-base-containing molecules are excellent candidates to promote color/fluorescence response depending on protonation and deprotonation. On the other hand, fluorescence must be guaranteed by a suited emissive core. Heterocyclic molecules [7,8,9,10,11,12,13,14,15,16,17,18,19,20,21], as one of the most important classes of compounds, can play the role of fluorogenic moiety. At present, one of the major problems encountered in optimizing optical sensors is achieving the right balance between the presence of the fluorogenic unit (soluble in organic solvents) and the water solubility. Water-soluble pH sensors may provide a diagnostic tool [22,23] by measuring intracellular pH gradient and evaluating the differences in pH between normal and altered cells.
Recently, we designed and synthesized an amphiphilic picolinoyl-hydrazone-based sensor [24] with a charged side chain capable of guaranteeing water solubility. In that study, we checked the effect of the heterocycle on the spectroscopic response involving pH determination.
In the present work, we explored the potential of a benzothiazole moiety, one of the most widely known heterocycles, in the production of an optical pH sensor. A new highly water-soluble, 5-(4-((2-(benzothiazole-2-carbonyl)hydrazono)methyl)-3-hydroxyphenoxy)-N,N,N-trimethylpentan-1-aminium bromide (BTABr) fluorescent and colorimetric probe was easily obtained by condensation reaction (see Scheme 1). In the keto-enolic tautomeric equilibrium, form B of the sensor undergoes two deprotonation steps, turning to forms C and finally D, respectively, by increasing pH parameter. Variation of pH includes a variation in the electronic state of the molecule and consequently in the absorbance/emission spectra. A full characterization of the probe was performed by NMR spectroscopy, elemental analysis, mass spectrometry measurements, DSC/TGA methods, and absorption and emission titrations in the pH range of 2.0–11.0. Single-crystal X-ray analysis of the BTABr probe clarified the structural aspects.

2. Experimental Section

All starting products were commercially available. 5-(4-Formyl-3-hydroxyphenoxy)-N,N,N-trimethylpentan-1-aminium bromide [24] was obtained as described in the literature. Benzothiazole-2-carboxylic acid hydrazide [25] was obtained by common synthetic route placing ethyl benzothiazole-2-carboxylate under reflux in ethanol with an excess of hydrazine for 1 h.
Optical microscopy was carried out with a Zeiss Axioscope polarizing microscope (Carl Zeiss, Oberkochen, Germany) equipped with a FP90 Mettler hot stage (Mettler-Toledo, LLC - Columbus, OH, USA). The decomposition temperature (Td), assumed as the temperature where is recorded the 5% weight loss, was determined by thermogravimetric analysis in air, using a PerkinElmer TGA 4000 (PerkinElmer, Inc., Waltham, MA, USA). Phase transition temperatures and enthalpies were measured using a PerkinElmer Pyris 1 DSC scanning calorimeter (PerkinElmer, Inc., Waltham, MA, USA) at a scanning rate of 10 °C/min, under nitrogen flow. 1H NMR and 13C NMR spectra were recorded with a Bruker 400 MHz apparatus (Bruker Corporation, Billerica, MA, USA). Ultraviolet–visible (UV–Vis) and fluorescence spectra were recorded with JASCO spectrometers (JASCO Inc., Mary’s Court, Easton, MD, USA).

2.1. Synthesis of BTABr

A 0.137 g (1.00 mmol) amount of benzothiazole-2-carboxylic acid hydrazide was added to 15 mL of a methanol solution containing 0.345 g (1.00 mmol) of 5-(4-formyl-3-hydroxyphenoxy)-N,N,N-trimethylpentan-1-aminium bromide. The reaction mixture was refluxed for 30 min then was cooled to 0 °C. Under this condition, a pale yellow crystalline solid precipitates. The solid was recovered by filtration and crystallized from methanol. Yield: 80%. Mp = 252 °C, ΔH = 84.69 J/g. Decomposition temperature: 293 °C. 1H NMR (400 MHz, DMSO-d6, 25 °C): δ = 12.82 (s, 1H), 11.26 (s, 1H), 8.73 (s, 1H), 8.24 (d, 4.2 Hz, 1H), 8.17 (d, 4.3 Hz, 1H), 7.65 (t, 5.4 Hz, 1H), 7.60 (t, 6.8 Hz, 1.7 Hz, 1H), 7.41 (d, 7.0 Hz, 1H), 6.49 (d, 6.9 Hz, 1H), 6.48 (s, 1H), 4.00 (t, 6.6 Hz, 2H), 3.28 (m, 2H), 2.47 (s, 9H), 1.74 (m, 4H), 1.40 (m, 2H) ppm. 13C NMR (400 MHz, DMSO-d6, 25 °C): δ = 163.7, 162.3, 160.0, 156.3, 153.2, 151.7, 136.5, 131.8, 127.8, 127.6, 124.6, 123.5, 112.1, 107.6, 102.1, 67.8, 65.7, 52.7, 28.4, 22.8, 22.2.
HRMS(ESI): m/z calculated for C23H29N4O3S+: 441.566; found 441.193 [M]+.
Elemental analysis calculated (%) for C23H29N4O3SBr: C, 52.96; H, 5.61; N, 10.74; found: C, 52.93; H, 5.67; N, 10.70.

2.2. X-ray Crystallography

X-ray crystal structure analysis was carried out on single crystals of BTABr obtained from slow evaporation of acetone/water solution at room temperature. One selected crystal was mounted in flowing N2 at 173 K on a Bruker-Nonius KappaCCD diffractometer equipped with an Oxford Cryostream apparatus (graphite monochromated MoKα radiation, λ = 0.71073 Å, CCD rotation images, thick slices, φ and ω scans to fill asymmetric unit). The structure was solved by direct methods (SIR97 program [26]) and anisotropically refined by the full matrix least-squares method on F2 against all independent measured reflections using SHELXL-2018/3 [27] and WinGX software [28]. In BTABr crystallization, H2O is present. The H atoms bound to N(amide), to the hydroxy group, and to the O(water) were located in different Fourier maps and their coordinates were refined with Uiso(H) equal to 1.2Ueq of the carrier atom. Some constraints were introduced in the last stage of refinement to regularize some H2O hydrogens (DFIX command of SHELXL). All the other hydrogen atoms were introduced in calculated positions and refined according to a riding model (C–H distances equal to 0.95–0.99 Å and Uiso(H) equal to 1.2Ueq of the carrier atom). Crystals resulted in being twinned (−1 0 0, 0 −1 0, −1 0 1 twinning law), no merging of reflections was made, and HKLF5 command was used. Crystal data and structure refinement details are reported in Table 1. Hydrogen bond geometry is reported in Table 2. The figures were generated using the ORTEP-3 [28] and Mercury CSD 4.0 [29] programs.
Crystal data were deposited at the Cambridge Crystallographic Data Centre with assigned number CCDC 1954197. These data can be obtained free of charge from www.ccdc.cam.ac.uk/data_request/cif.

2.3. UV–Vis and Fluorescence pH Titrations and Naked-Eye Detection

Stock solutions (1.00M) of Britton–Robinson buffer (at 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, and 11.0 pH value) were prepared in bidistilled water (pH 6.25) as described in the literature [30]. Aqueous solutions of BTABr (100 μM and 20 μM, respectively), from bidistilled water, were prepared for fluorescence and absorbance titrations. The probe titration was performed by adding 60 μL of the buffer stock solutions to 2.5 mL of BTABr dissolved in the solvent. After mixing for a few seconds, the absorption or fluorescence spectra were recorded at room temperature.
For the naked-eye detection, solutions of 200 μM of BTABr in 1.00 M buffer of pH 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, and 11.0 were prepared. The laboratory paper soaked using a 0.01 M water solution of the probe was Labor filter paper, 67 g/m2, minimum porosity. The samples were immediately photographed.

3. Results and Discussion

3.1. Characterization and Sensing Ability of BTABr

Compound BTABr was obtained as summarized in Scheme 1 by condensation reaction. 1H-NMR and 13C‑NMR were used to identify the compounds and evaluate their degree of purity. Phase behavior was examined by optical observation as well as DSC and TGA analysis. The probe shows a large range of stability after melting (at 252 °C) and no decomposition is detected until about 293 °C in air, making it a good candidate for its stability.
Remarkably, it was not necessary to employ mixed organic solvent–water solutions [31] because of the excellent water solubility of the probe (0.12 M in bidistilled water at pH = 6.25 for the protonated form). All photophysical measurements were performed in water solution or water–ethanol (8:2), the addition of ethanol slightly increasing emission intensity. The fluorescence quantum yield was measured in water for the deprotonated form D at pH = 11.0 by relative methods using quinine sulfate (QS) as standard (0.546 in H2SO4 1 N, excited at 365 nm) according to [32,33,34]. The value of 6.20% is comparable to that reported for water-soluble pH probes [35,36]. In more concentrated solutions, the emission increases become perceptible to the naked eye (see Section 2.3).
In the UV–Vis experiments, the absorption maximum was recorded at about 351 nm for the chromophore in the protonated form (at pH from 2.0 to 6.0). In the pH range from 8.0 to 11.0, the peak undergoes a relevant red shift to about 425 nm, consistent with the gradual deprotonation of the tautomeric form B to the deprotonated forms C and then D in increasingly basic media. At pH = 7.0, the absorbance pattern reveals two peaks at 344 nm and at 425 nm. This behavior is evident in Figure 1 (on the left) where the titration in the 100 μM water solution of BTABr is reported. The acidic solutions are pale yellow and the broad absorption band at about 425 nm is responsible for the bright yellow color of the basic solutions. The naked-eye response of the same solutions is quite evident (see Figure 2, solutions in natural light). The net color change from acidic to alkaline forms is observable also when the probe is adsorbed on laboratory paper (Figure 2, paper disc, upper half).
The diagram of the fluorescence titration performed using the 20 μM solution of BTABr in water is reported in Figure 1 on the right. The emission was recorded as a band peaked at 517 nm which increases and sharpens from the 2.0 to 11.0 pH values. The acid solutions are much less intense in emission with respect to the basic ones, as can be observed with the naked eye in Figure 2 (solutions photographed in UV–Vis light). The same behavior is shown by the soaked-on paper sample (Figure 2, paper disc, lower half). Under the UV–Vis lamp, the deprotonated samples give a more intense yellow emission with respect to the quite colorless protonated form.
Finally, high Stokes shifts were evaluated in the basic range (92 nm), becoming even larger in the acidic solution (166 nm). Replicability and reversibility were positively checked by repeatedly turning on and off absorbance/fluorescence real-time response by NaOH or HCl addition in the fluorescence/absorbance experiments.

3.2. Crystal Structure of BTABr

A complete XRD analysis was performed on the single crystals of the BTABr probe obtained from slow evaporation of the acetone/water solution at room temperature. BTABr crystallizes in the triclinic P -1 space group with one BTA+ cation, one bromine anion, and three lattice water molecules contained in the independent unit. All bond lengths and angles are in the normal range. Crystal data and structural details are reported in Table 1. The hydrogen bonding geometry is reported in Table 2. The ORTEP view of the molecular structure is reported in Figure 3.
The pattern of bond lengths in the compound agrees with the more stable keto form A of Scheme 1; in particular, the typical carbonyl double bond distance of 1.219(9) Å is found for the C8-O1 bond. The presence of the N(amide) hydrogen atom is proved by its localization in different Fourier maps. The existence of an intramolecular hydrogen bond between the OH donor and the N(imine) acceptor groups locks the phenol-azo group into a planar conformation (Table 2). In the side benzothiazole group, the N(thiazole) is cis-disposed to the N(amide) atom. A slight inclination of the benzotriazole group with respect to the phenol ring is observed, mainly due to the torsion around the C7-C8 bond (N1-C7-C8-N2 = 7.3(11)°). The side oxoalkylamino group is in the all-trans conformation and in plane with the attached phenol group, except for the terminal -CH2-N(CH3)3 group that protrudes out of the mean plane of the molecule as a consequence of the torsion angle around the C18-C19 bond (C17-C18-C19-C20 = −68.3(9)°). In this way a flat, elongated hooked shape of the molecular cation is achieved (Figure 3).
The crystal packing is stabilized by intermolecular hydrogen bonds that involve the strong donor/acceptor groups of the molecule, the lattice water molecules, and the bromine anion in a complex tridimensional H-bonding pattern. In the molecule, the imine group acts as donor towards a water molecule and the hydroxy group acts as acceptor from another water molecule. The lattice water molecules are also involved in hydrogen bonds with the acceptor bromine anion to form an H-bonded ring across a crystallographic inversion center having an R4,6(12) graph set (Figure 4). In the crystal packing, the molecules are joined through H-bonded water molecules in an antiparallel stacking at about 3.4 Å, with benzothiazole and alkyl groups of adjacent molecules facing each other and adjacent phenol rings slipped to each other.
The Hirshfeld surfaces and two-dimensional fingerprint plots using CrystalExplorer 17.5 [37] were utilized to analyze the close intermolecular contacts. The presence of hydrogen bonds was confirmed by the red spots on the Hirshfeld surface mapped over dnorm (Figure 5, left) and by the bidimensional fingerprint plot (Figure 5, right).
In the Hirshfeld fingerprint plot (Figure 5, right), the intermolecular O···H/H···O (12.9%) and Br…H/H…Br (10.8%) interactions are displayed as a pair of sharp spikes symmetrically disposed with respect to the diagonal. As expected, a large part of H···H (46.2%) interactions are found (diagonal blue strip that ends at about di = de = 1.1 Å). A small green area at about di + de = 3.4 Å with a small amount of C…C (2.7%) interactions is diagnostic of few π···π interactions between molecules, due to their antiparallel stacking with prevalent contacts between alkyl and aromatic groups.

4. Conclusions

The BTABr probe shows a remarkable pH-dependent behavior both in absorbance and in emission and a net on-off switch effect at neutral pH. The effect is clearly perceivable even to the naked eye in solution and on the soaked-on paper sample. Common drawbacks of many pH probes, such as scarce stability, replicability and reversibility, slow response, and narrow Stokes shifts do not affect BTABr. Moreover, the sensor is so soluble in water that it can be dissolved without any addition of organic solvents. Based on these characteristics, BTABr is a good candidate as on-off colorimetric and fluorescence real-time pH sensor, potentially employable for biological determinations.

Author Contributions

For research articles with several authors, a short paragraph specifying their individual contributions must be provided. The following statements should be used U.C., B.P., and R.D. conceptualized and designed the experiments; R.D., and A.T. performed the experiments; R.D. and B.P. analyzed the data; U.C. methodology, resources and wrote the paper. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by by the Italian Ministry of Education, University and Research (MIUR) (Piano Lauree Scientifiche “Scienza dei Materiali” 2016–2018).

Conflicts of Interest

The authors declare no conflict of interest.

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Crystals 10 00083 sch001 550
Scheme 1. Synthetic route to the target compound BTABr in the two tautomeric forms (A and B) at pH acidic-neutral. In red, the more acid proton (lost in the C form) and, in green, the second acid proton (lost in the D form).
Scheme 1. Synthetic route to the target compound BTABr in the two tautomeric forms (A and B) at pH acidic-neutral. In red, the more acid proton (lost in the C form) and, in green, the second acid proton (lost in the D form).
Crystals 10 00083 sch001
Crystals 10 00083 g001 550
Figure 1. UV–Visible (UV–Vis) spectral titration of BTABr 100 μM in water using Britton–Robinson buffers from pH = 2.0 to pH = 11.0 (on the left). Fluorescence spectral titration of BTABr 20 μM in water using Britton–Robinson buffers from pH = 2.0 to pH = 11.0 (excitation wavelength of 350 nm).
Figure 1. UV–Visible (UV–Vis) spectral titration of BTABr 100 μM in water using Britton–Robinson buffers from pH = 2.0 to pH = 11.0 (on the left). Fluorescence spectral titration of BTABr 20 μM in water using Britton–Robinson buffers from pH = 2.0 to pH = 11.0 (excitation wavelength of 350 nm).
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Figure 2. On the left: color of 100 μM BTABr probe in buffer of pH = 4.0, pH = 7.0, and pH = 9.0 photographed under visible light (above) and UV lamp at 365 nm (below). On the right: color of laboratory paper soaked in the probe at pH = 4.0 (on the left) and pH = 9.0 (right) photographed at visible light (above) and under UV lamp at 365 nm (down).
Figure 2. On the left: color of 100 μM BTABr probe in buffer of pH = 4.0, pH = 7.0, and pH = 9.0 photographed under visible light (above) and UV lamp at 365 nm (below). On the right: color of laboratory paper soaked in the probe at pH = 4.0 (on the left) and pH = 9.0 (right) photographed at visible light (above) and under UV lamp at 365 nm (down).
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Figure 3. UP: Ortep view of BTABr normal to phenol ring with the H bonding pattern of H2O molecules drawn as dashed lines. DOWN: view of BTA+ cation down the C11-C15 line. Thermal ellipsoids are drawn at 50% probability level.
Figure 3. UP: Ortep view of BTABr normal to phenol ring with the H bonding pattern of H2O molecules drawn as dashed lines. DOWN: view of BTA+ cation down the C11-C15 line. Thermal ellipsoids are drawn at 50% probability level.
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Figure 4. Partial packing showing the H-bonding pattern. Intra- and intermolecular hydrogen bonds are drawn as cyan dashed lines.
Figure 4. Partial packing showing the H-bonding pattern. Intra- and intermolecular hydrogen bonds are drawn as cyan dashed lines.
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Figure 5. Left: Two-dimensional full fingerprint plot of BTABr. Significative contacts: H…H (46.2%), O…H/H…O (12.9%), Br…H/H…Br (10.8%), and C…C (2.7%). Right: Hirshfeld surface mapped over dnorm.
Figure 5. Left: Two-dimensional full fingerprint plot of BTABr. Significative contacts: H…H (46.2%), O…H/H…O (12.9%), Br…H/H…Br (10.8%), and C…C (2.7%). Right: Hirshfeld surface mapped over dnorm.
Crystals 10 00083 g005
Table
Table 1. Crystal data and structure refinement details for BTABr.
Table 1. Crystal data and structure refinement details for BTABr.
CCDC Number1954197
Empirical formulaC23 H35 Br N4 O6 S
Formula weight575.52
T (K)173(2)
λ (Å)0.71073
Crystal systemTriclinic
Space groupP21/c
a (Å)8.205(4)
b (Å)12.931(4)
c (Å)13.086(7)
a (°)88.71(7)
β (°)74.22(6)
γ (°)81.81(4)
V (Å3)1322.3(11)
Z2
Dcalc (Mg/m3)1.446
μ (mm−1)1.678
F(000)600
θ range (°)2.2527.497
Reflections collected/unique18815/18815
Data/restraints/parameters18815/10/340
Goodness-of-fit on F21.165
Final R, wR indices [I > 2s(I)]0.0623, 0.1639
Final R, wR indices (all data)0.0859, 0.1895
Largest diff. peak / hole (eA−3)1.959 / −0.869
Table
Table 2. Hydrogen bonding geometry for BTABr (e.s.d. values are in parentheses).
Table 2. Hydrogen bonding geometry for BTABr (e.s.d. values are in parentheses).
D–H⋯A d(D–H) Åd(H···A) Åd(D⋯A) Å<(D–H⋯A) °
C(9)-H(9)...O(6) (i)0.952.563.333(10)139.0
C(16)-H(16B)...O (ii)0.992.613.570(10)163.3
C(21)-H(21B)...O(1) (iii)0.982.353.236(10)150.3
C(23)-H(23B)...O(1) (iii)0.982.373.235(11)147.4
N(2)-H(2A)...O(6) (i)0.881.982.829(9)161.9
O(2)-H(2B)...N(3)0.73(9)1.98(9)2.578(8)139(10)
O(4)-H(4A)...Br(1) (iv)0.832(18)2.59(3)3.399(6)163(8)
O(4)-H(4B)...O(2) 0.833(18)2.02(3)2.813(8)158(7)
O(5)-H(5A)...O(4)0.842(18)1.97(2)2.814(8)175(7)
O(6)-H(6A)...O(5)0.848(18)1.89(2)2.733(8)173(8)
O(6)-H(6B)...Br(1) (ii)0.841(18)2.514(19)3.352(6)174(7)
Symmetry code: (i) = −x + 1, −y + 1, −z; (ii) = −x, −y + 1, −z + 1; (iii) = x − 1, y + 1,z; (iv) = −x + 1, −y + 1, −z + 1;.

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Diana, R.; Caruso, U.; Tuzi, A.; Panunzi, B. A Highly Water-Soluble Fluorescent and Colorimetric pH Probe. Crystals 2020, 10, 83. https://doi.org/10.3390/cryst10020083

AMA Style

Diana R, Caruso U, Tuzi A, Panunzi B. A Highly Water-Soluble Fluorescent and Colorimetric pH Probe. Crystals. 2020; 10(2):83. https://doi.org/10.3390/cryst10020083

Chicago/Turabian Style

Diana, Rosita, Ugo Caruso, Angela Tuzi, and Barbara Panunzi. 2020. "A Highly Water-Soluble Fluorescent and Colorimetric pH Probe" Crystals 10, no. 2: 83. https://doi.org/10.3390/cryst10020083

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