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Methods for Obtaining Better Diffractive Protein Crystals: From Sample Evaluation to Space Crystallization

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Confocal Science Inc. 2-12-2 Iwamoto-cho, Chiyoda-ku, Tokyo 101-0023, Japan
2
Maruwa Foods and Biosciences Inc. 170-1 Tsutsui-cho, Yamato-Koriyama, Nara 639-1123, Japan
*
Author to whom correspondence should be addressed.
Crystals 2020, 10(2), 78; https://doi.org/10.3390/cryst10020078
Received: 13 December 2019 / Revised: 28 January 2020 / Accepted: 29 January 2020 / Published: 30 January 2020
(This article belongs to the Special Issue Protein Crystallography)
In this paper, we present a summary on how to obtain protein crystals from which better diffraction images can be produced. In particular, we describe, in detail, quality evaluation of the protein sample, the crystallization conditions and methods, flash-cooling protection of the crystal, and crystallization under a microgravity environment. Our approach to protein crystallization relies on a theoretical understanding of the mechanisms of crystal growth. They are useful not only for space experiments, but also for crystallization in the laboratory. View Full-Text
Keywords: protein crystallization; protein sample evaluation; JAXA PCG; microgravity protein crystallization; protein sample evaluation; JAXA PCG; microgravity
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MDPI and ACS Style

Hashizume, Y.; Inaka, K.; Furubayashi, N.; Kamo, M.; Takahashi, S.; Tanaka, H. Methods for Obtaining Better Diffractive Protein Crystals: From Sample Evaluation to Space Crystallization. Crystals 2020, 10, 78. https://doi.org/10.3390/cryst10020078

AMA Style

Hashizume Y, Inaka K, Furubayashi N, Kamo M, Takahashi S, Tanaka H. Methods for Obtaining Better Diffractive Protein Crystals: From Sample Evaluation to Space Crystallization. Crystals. 2020; 10(2):78. https://doi.org/10.3390/cryst10020078

Chicago/Turabian Style

Hashizume, Yoshinobu; Inaka, Koji; Furubayashi, Naoki; Kamo, Masayuki; Takahashi, Sachiko; Tanaka, Hiroaki. 2020. "Methods for Obtaining Better Diffractive Protein Crystals: From Sample Evaluation to Space Crystallization" Crystals 10, no. 2: 78. https://doi.org/10.3390/cryst10020078

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