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Open AccessArticle

Tailoring Celluclast® Cocktail’s Performance towards the Production of Prebiotic Cello-Oligosaccharides from Waste Forest Biomass

Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 971 87 Luleå, Sweden
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Author to whom correspondence should be addressed.
These authors equally contributed to this work.
Catalysts 2019, 9(11), 897; https://doi.org/10.3390/catal9110897
Received: 22 September 2019 / Revised: 25 October 2019 / Accepted: 25 October 2019 / Published: 28 October 2019
(This article belongs to the Special Issue Environmental Biocatalysis: From Remediation to Waste Valorization)
The main objective of this study focused on the sustainable production of cellobiose and other cellulose-derived oligosaccharides from non-edible sources, more specifically, from forest residues. For this purpose, a fine-tuning of the performance of the commercially available enzyme mixture Celluclast® was conducted towards the optimization of cellobiose production. By enzyme reaction engineering (pH, multi-stage hydrolysis with buffer exchange, addition of β-glucosidase inhibitor), a cellobiose-rich product with a high cellobiose to glucose ratio (37.4) was achieved by utilizing organosolv-pretreated birch biomass. In this way, controlled enzymatic hydrolysis combined with efficient downstream processing, including product recovery and purification through ultrafiltration and nanofiltration, can potentially support the sustainable production of food-grade oligosaccharides from forest biomass. The potential of the hydrolysis product to support the growth of two Lactobacilli probiotic strains as a sole carbon source was also demonstrated. View Full-Text
Keywords: non-digestible oligosaccharides; Celluclast®; cellobiose; conduritol-B-epoxide; prebiotic; lignocellulose enzyme hydrolysis non-digestible oligosaccharides; Celluclast®; cellobiose; conduritol-B-epoxide; prebiotic; lignocellulose enzyme hydrolysis
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Karnaouri, A.; Matsakas, L.; Bühler, S.; Muraleedharan, M.N.; Christakopoulos, P.; Rova, U. Tailoring Celluclast® Cocktail’s Performance towards the Production of Prebiotic Cello-Oligosaccharides from Waste Forest Biomass. Catalysts 2019, 9, 897.

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