Tailoring Celluclast® Cocktail’s Performance towards the Production of Prebiotic Cello-Oligosaccharides from Waste Forest Biomass
Abstract
:1. Introduction
2. Results
2.1. Effect of β-Glucosidase Inhibitor
2.2. Effect of Enzyme and Conduritol Mixture Preincubation
2.3. Effect of pH and Enzyme Loading
2.4. Effect of Hydrolysis Time, Inhibitor Concentration and Reaction Temperature at pH 7.0
2.5. Effect of Buffer Exchange, Enzyme and Inhibitor Supplementation
2.6. Evaluation of LPMO on the Production of Cellobiose
2.7. Evaluation of Different Substrates
2.8. Scale-up Reaction and Downstream Processing for Product Recovery
2.9. Evaluation of COS Prebiotic Activity
Growth Potential of Lactobacillus Strains on Pure Cellobiose and Birch-Derived Sugars
3. Discussion
4. Materials and Methods
4.1. Enzymes and Substrates
4.2. Hydrolysis of Lignocellulosic Materials
4.3. Evaluation of Synergistic Effect of PcLPMO9D with Cellulases towards Cellobiose Production
4.4. Scale-up Hydrolysis Reaction and Product Recovery
4.5. Determination of Prebiotic Potential of Birch Hydrolysate
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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24 h | 48 h | |||||
---|---|---|---|---|---|---|
CB (% w/w) | Glu (% w/w) | CB:Glu | CB (% w/w) | Glu (% w/w) | CB:Glu | |
No preincubation | 17.7 ± 0.9 | 2.7 ± 0.1 | 6.7 | 19.5 ± 1.1 | 3.0 ± 1.0 | 6.5 |
Preincubation | 17.0 ± 1.1 | 2.9 ± 0.0 | 5.8 | 19.9 ± 1.0 | 3.4 ± 1.1 | 5.8 |
Temperature/Incubation Time | CB | Glu | CB:Glu | mg CB/g Substrate |
---|---|---|---|---|
50 °C, 12 h | 13.0 ± 1.1 | 0.5 ± 0.1 | 27.1 | 111.97 |
50 °C, 24 h | 16.3 ± 0.5 | 0.6 ± 0.1 | 28.0 | 140.66 |
45 °C, 12 h | 16.7 ± 0.7 | 1.4 ± 0.1 | 12.1 | 143.97 |
45 °C, 24 h | 20.1 ± 0.4 | 1.2 ± 0.0 | 17.3 | 173.45 |
40 °C, 12 h | 12.8 ± 1.0 | 1.3 ± 0.2 | 9.6 | 110.64 |
40 °C, 24 h | 18.5 ± 0.2 | 1.7 ± 0.2 | 10.7 | 159.4 |
Conditions | CB (% w/w) | Glu (% w/w) | CB:Glu | mg CB/g Substrate | % CB Increase | % Glu Increase |
---|---|---|---|---|---|---|
25 mg enzyme/g sub | 16.0 ± 1.7 | 0.7 ± 0.1 | 21.8 | 145.7 | 0 | 0 |
25 mg enzyme/g sub + 2.5 mg PcLPMO9D/g sub | 24.3 ± 1.2 | 1.9 ± 0.1 | 13.1 | 220.9 | 51.5 | 151.6 |
27.5 mg enzyme/g sub | 16.0 ± 1.0 | 0.7 ± 1.0 | 21.8 | 145.4 | 0 | 0 |
L. gasseri | L. plantarum | |||||||
---|---|---|---|---|---|---|---|---|
Cellobiose | Birch Hydrolysate | Cellobiose | Birch Hydrolysate | |||||
0 h | 77 h | 0 h | 77 h | 0 h | 23 h | 0 h | 23 h | |
cellobiose | 19.7 ± 1.1 | 0.1 ± 0.0 | 19.6 ± 2.0 | 0.0 ± 0.00 | 20.1 ± 0.06 | 0.0 ± 0.0 | 20.0 ± 2.6 | 0.1 ± 0.1 |
lactic acid | 0.1 ± 0.1 | 8.5 ± 1.4 | 0.3 ± 0.1 | 39.5 ± 3.9 | 0.4 ± 0.01 | 21.1 ± 2.9 | 1.2 ± 0.2 | 37.1 ± 2.9 |
acetic acid | 4.0 ± 1.3 | 4.1 ± 0.0 | 4.2 ± 0.8 | 4.4 ± 1.0 | 4.3 ± 0.25 | 3.8 ± 0.6 | 4.5 ± 1.7 | 4.4 ± 1.9 |
propionic acid | 0.9 ± 0.0 | 1.03 ± 0.7 | 1.1 ± 0.0 | 0.8 ± 0.2 | 0.9 ± 0.12 | 0.9 ± 0.0 | 1.1 ± 0.7 | 0.8 ± 0.2 |
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Karnaouri, A.; Matsakas, L.; Bühler, S.; Muraleedharan, M.N.; Christakopoulos, P.; Rova, U. Tailoring Celluclast® Cocktail’s Performance towards the Production of Prebiotic Cello-Oligosaccharides from Waste Forest Biomass. Catalysts 2019, 9, 897. https://doi.org/10.3390/catal9110897
Karnaouri A, Matsakas L, Bühler S, Muraleedharan MN, Christakopoulos P, Rova U. Tailoring Celluclast® Cocktail’s Performance towards the Production of Prebiotic Cello-Oligosaccharides from Waste Forest Biomass. Catalysts. 2019; 9(11):897. https://doi.org/10.3390/catal9110897
Chicago/Turabian StyleKarnaouri, Anthi, Leonidas Matsakas, Saskja Bühler, Madhu Nair Muraleedharan, Paul Christakopoulos, and Ulrika Rova. 2019. "Tailoring Celluclast® Cocktail’s Performance towards the Production of Prebiotic Cello-Oligosaccharides from Waste Forest Biomass" Catalysts 9, no. 11: 897. https://doi.org/10.3390/catal9110897