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Open AccessArticle

Chitosan–Collagen Coated Magnetic Nanoparticles for Lipase Immobilization—New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid

1
Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, Poland
2
Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, dr A. Jurasza 2, 85-089 Bydgoszcz, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: David D. Boehr
Catalysts 2017, 7(1), 26; https://doi.org/10.3390/catal7010026
Received: 27 October 2016 / Revised: 6 January 2017 / Accepted: 10 January 2017 / Published: 14 January 2017
(This article belongs to the Special Issue Immobilized Enzymes: Strategies for Enzyme Stabilization)
This article presents a novel route for crosslinking a polysaccharide and polysaccharide/protein shell coated on magnetic nanoparticles (MNPs) surface via condensation reaction with squaric acid (SqA). The syntheses of four new types of collagen-, chitosan-, and chitosan–collagen coated magnetic nanoparticles as supports for enzyme immobilization have been done. Structure and morphology of prepared new materials were characterized by attenuated total reflectance Fourier-transform infrared (ATR-FTIR), XRD, and TEM analysis. Next, the immobilization of lipase from Candida rugosa was performed on the nanoparticles surface via N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC)/N-hydroxy-succinimide (NHS) mechanism. The best results of lipase activity recovery and specific activities were observed for nanoparticles with polymer shell crosslinked via a novel procedure with squaric acid. The specific activity for lipase immobilized on materials crosslinked with SqA (52 U/mg lipase) was about 2-fold higher than for enzyme immobilized on MNPs with glutaraldehyde addition (26 U/mg lipase). Moreover, a little hyperactivation of lipase immobilized on nanoparticles with SqA was observed (104% and 112%). View Full-Text
Keywords: immobilization; lipase; magnetic nanoparticles; chitosan; collagen; squaric acid immobilization; lipase; magnetic nanoparticles; chitosan; collagen; squaric acid
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MDPI and ACS Style

Ziegler-Borowska, M.; Chelminiak-Dudkiewicz, D.; Siódmiak, T.; Sikora, A.; Wegrzynowska-Drzymalska, K.; Skopinska-Wisniewska, J.; Kaczmarek, H.; Marszałł, M.P. Chitosan–Collagen Coated Magnetic Nanoparticles for Lipase Immobilization—New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid. Catalysts 2017, 7, 26. https://doi.org/10.3390/catal7010026

AMA Style

Ziegler-Borowska M, Chelminiak-Dudkiewicz D, Siódmiak T, Sikora A, Wegrzynowska-Drzymalska K, Skopinska-Wisniewska J, Kaczmarek H, Marszałł MP. Chitosan–Collagen Coated Magnetic Nanoparticles for Lipase Immobilization—New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid. Catalysts. 2017; 7(1):26. https://doi.org/10.3390/catal7010026

Chicago/Turabian Style

Ziegler-Borowska, Marta; Chelminiak-Dudkiewicz, Dorota; Siódmiak, Tomasz; Sikora, Adam; Wegrzynowska-Drzymalska, Katarzyna; Skopinska-Wisniewska, Joanna; Kaczmarek, Halina; Marszałł, Michał P. 2017. "Chitosan–Collagen Coated Magnetic Nanoparticles for Lipase Immobilization—New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid" Catalysts 7, no. 1: 26. https://doi.org/10.3390/catal7010026

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