Next Article in Journal
High Catalytic Efficiency of a Layered Coordination Polymer to Remove Simultaneous Sulfur and Nitrogen Compounds from Fuels
Next Article in Special Issue
Enzymatic Synthesis of Estolides from Castor Oil
Previous Article in Journal
Catalytic and Electrochemical Properties of Ag Infiltrated Perovskite Coatings for Propene Deep Oxidation
Previous Article in Special Issue
A Three-Step Process for the Bioconversion of Whey Permeate into a Glucose-Free D-Tagatose Syrup
 
 
Article

Penicillin Acylase from Streptomyces lavendulae and Aculeacin A Acylase from Actinoplanes utahensis: Two Versatile Enzymes as Useful Tools for Quorum Quenching Processes

1
Department of Biochemistry and Molecular Biology, Faculty of Biology, Complutense University (UCM), José Antonio Novais 12, 28040 Madrid, Spain
2
Department of Environmental Biology, Biological Research Center (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain
3
Department of Genetics, Physiology and Microbiology, Faculty of Biology, UCM, José Antonio Novais 12, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Present address: Roche Diagnostics GmbH, Nonnenwald 2, 82377 Penzberg, Germany.
Present address: Applied Biotechnology Group, European University of Madrid, Tajo, s/n. Urb. El Bosque, 28670 Villaviciosa de Odón, Madrid, Spain.
§
Present address: Laboratory of Cell and Developmental Signaling, NCI ATRF, Frederick, MD 21701, USA.
Present address: Screening and Compound Profiling Institut de Recherches Servier, 125 Chemin de Ronde, 78290 Croissy-sur-Seine, France.
These two researchers share the position of last author.
Catalysts 2020, 10(7), 730; https://doi.org/10.3390/catal10070730
Received: 3 June 2020 / Revised: 23 June 2020 / Accepted: 26 June 2020 / Published: 1 July 2020
Many Gram-negative bacteria produce N-acyl-homoserine lactones (AHLs), quorum sensing (QS) molecules that can be enzymatically inactivated by quorum quenching (QQ) processes; this approach is considered an emerging antimicrobial alternative. In this study, kinetic parameters of several AHLs hydrolyzed by penicillin acylase from Streptomyces lavendulae (SlPA) and aculeacin A acylase from Actinoplanes utahensis (AuAAC) have been determined. Both enzymes catalyze efficiently the amide bond hydrolysis in AHLs with different acyl chain moieties (with or without 3-oxo modification) and exhibit a clear preference for AHLs with long acyl chains (C12-HSL > C14-HSL > C10-HSL > C8-HSL for SlPA, whereas C14-HSL > C12-HSL > C10-HSL > C8-HSL for AuAAC). Involvement of SlPA and AuAAC in QQ processes was demonstrated by Chromobacterium violaceum CV026-based bioassays and inhibition of biofilm formation by Pseudomonas aeruginosa, a process controlled by QS molecules, suggesting the application of these multifunctional enzymes as quorum quenching agents, this being the first time that quorum quenching activity was shown by an aculeacin A acylase. In addition, a phylogenetic study suggests that SlPA and AuAAC could be part of a new family of actinomycete acylases, with a preference for substrates with long aliphatic acyl chains, and likely involved in QQ processes. View Full-Text
Keywords: penicillin acylase; aculeacin acylase; N-acyl-homoserine lactone acylases; quorum quenching; biofouling penicillin acylase; aculeacin acylase; N-acyl-homoserine lactone acylases; quorum quenching; biofouling
Show Figures

Figure 1

MDPI and ACS Style

Velasco-Bucheli, R.; Hormigo, D.; Fernández-Lucas, J.; Torres-Ayuso, P.; Alfaro-Ureña, Y.; Saborido, A.I.; Serrano-Aguirre, L.; García, J.L.; Ramón, F.; Acebal, C.; Santos, A.; Arroyo, M.; de la Mata, I. Penicillin Acylase from Streptomyces lavendulae and Aculeacin A Acylase from Actinoplanes utahensis: Two Versatile Enzymes as Useful Tools for Quorum Quenching Processes. Catalysts 2020, 10, 730. https://doi.org/10.3390/catal10070730

AMA Style

Velasco-Bucheli R, Hormigo D, Fernández-Lucas J, Torres-Ayuso P, Alfaro-Ureña Y, Saborido AI, Serrano-Aguirre L, García JL, Ramón F, Acebal C, Santos A, Arroyo M, de la Mata I. Penicillin Acylase from Streptomyces lavendulae and Aculeacin A Acylase from Actinoplanes utahensis: Two Versatile Enzymes as Useful Tools for Quorum Quenching Processes. Catalysts. 2020; 10(7):730. https://doi.org/10.3390/catal10070730

Chicago/Turabian Style

Velasco-Bucheli, Rodrigo, Daniel Hormigo, Jesús Fernández-Lucas, Pedro Torres-Ayuso, Yohana Alfaro-Ureña, Ana I. Saborido, Lara Serrano-Aguirre, José L. García, Fernando Ramón, Carmen Acebal, Antonio Santos, Miguel Arroyo, and Isabel de la Mata. 2020. "Penicillin Acylase from Streptomyces lavendulae and Aculeacin A Acylase from Actinoplanes utahensis: Two Versatile Enzymes as Useful Tools for Quorum Quenching Processes" Catalysts 10, no. 7: 730. https://doi.org/10.3390/catal10070730

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop