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Open AccessArticle

Application of Immobilized Cholest-4-en-3-one Δ1-Dehydrogenase from Sterolibacterium Denitrificans for Dehydrogenation of Steroids

1
Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239 Kraków, Poland
2
Faculty of Energy and Fuels, AGH University of Science and Technology, 30 A. Mickiewicza Av., 30-059 Kraków, Poland
3
Department of Chemical Engineering and Process Design, Silesian University of Technology, Ks. M. Strzody 7, 44-100 Gliwice, Poland
*
Author to whom correspondence should be addressed.
Catalysts 2020, 10(12), 1460; https://doi.org/10.3390/catal10121460
Received: 30 November 2020 / Revised: 11 December 2020 / Accepted: 12 December 2020 / Published: 14 December 2020
(This article belongs to the Special Issue Biocatalysis in Lipids Modification)
Cholest-4-en-3-one Δ1-dehydrogenase (AcmB) from Sterolibacterium denitrificans was successfully immobilized on 3-aminopropyltrimethoysilane functionalized mesoporous cellular foam (MCF) and Santa Barbara Amorphous (SBA-15) silica supports using adsorption or covalently with glutaraldehyde or divinyl sulfone linkers. The best catalyst, AcmB on MCF linked covalently with glutaraldehyde, retained the specific activity of the homogenous enzyme while exhibiting a substantial increase of the operational stability. The immobilized enzyme was used continuously in the fed-batch reactor for 27 days, catalyzing 1,2-dehydrogenation of androst-4-en-3-one to androst-1,4-dien-3-one with a final yield of 29.9 mM (8.56 g/L) and 99% conversion. The possibility of reuse of the immobilized catalyst was also demonstrated and resulted in a doubling of the product amount compared to that in the reference homogenous reactor. Finally, it was shown that molecular oxygen from the air can efficiently be used as an electron acceptor either reoxidizing directly the enzyme or the reduced 2,4-dichlorophenolindophenol (DCPIPH2). View Full-Text
Keywords: 3-ketosteroid Δ1-dehydrogenase; KSTD; KSDH; AcmB; 1,2-dehydrogenation; cholest-4-en-3-one Δ1-dehydrogenase; enzyme immobilization; FAD-dependent enzymes; enzyme immobilization 3-ketosteroid Δ1-dehydrogenase; KSTD; KSDH; AcmB; 1,2-dehydrogenation; cholest-4-en-3-one Δ1-dehydrogenase; enzyme immobilization; FAD-dependent enzymes; enzyme immobilization
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MDPI and ACS Style

Tataruch, M.; Wójcik, P.; Wojtkiewicz, A.M.; Zaczyk, K.; Szymańska, K.; Szaleniec, M. Application of Immobilized Cholest-4-en-3-one Δ1-Dehydrogenase from Sterolibacterium Denitrificans for Dehydrogenation of Steroids. Catalysts 2020, 10, 1460. https://doi.org/10.3390/catal10121460

AMA Style

Tataruch M, Wójcik P, Wojtkiewicz AM, Zaczyk K, Szymańska K, Szaleniec M. Application of Immobilized Cholest-4-en-3-one Δ1-Dehydrogenase from Sterolibacterium Denitrificans for Dehydrogenation of Steroids. Catalysts. 2020; 10(12):1460. https://doi.org/10.3390/catal10121460

Chicago/Turabian Style

Tataruch, Mateusz; Wójcik, Patrycja; Wojtkiewicz, Agnieszka M.; Zaczyk, Katarzyna; Szymańska, Katarzyna; Szaleniec, Maciej. 2020. "Application of Immobilized Cholest-4-en-3-one Δ1-Dehydrogenase from Sterolibacterium Denitrificans for Dehydrogenation of Steroids" Catalysts 10, no. 12: 1460. https://doi.org/10.3390/catal10121460

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