Next Article in Journal
Silencing of Taxol-Sensitizer Genes in Cancer Cells: Lack of Sensitization Effects
Next Article in Special Issue
Not All Next Generation Sequencing Diagnostics are Created Equal: Understanding the Nuances of Solid Tumor Assay Design for Somatic Mutation Detection
Previous Article in Journal
Involvement of 14-3-3 Proteins in Regulating Tumor Progression of Hepatocellular Carcinoma
Previous Article in Special Issue
Next-Generation Sequencing in Clinical Oncology: Next Steps Towards Clinical Validation
Open AccessReview

Exploring the Mechanisms of Gastrointestinal Cancer Development Using Deep Sequencing Analysis

Department of Gastroenterology and Hepatology, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Camile S. Farah and William Chi-shing Cho
Cancers 2015, 7(2), 1037-1051; https://doi.org/10.3390/cancers7020823
Received: 21 April 2015 / Accepted: 8 June 2015 / Published: 15 June 2015
(This article belongs to the Special Issue Next Generation Sequencing Approaches in Cancer)
Next-generation sequencing (NGS) technologies have revolutionized cancer genomics due to their high throughput sequencing capacity. Reports of the gene mutation profiles of various cancers by many researchers, including international cancer genome research consortia, have increased over recent years. In addition to detecting somatic mutations in tumor cells, NGS technologies enable us to approach the subject of carcinogenic mechanisms from new perspectives. Deep sequencing, a method of optimizing the high throughput capacity of NGS technologies, allows for the detection of genetic aberrations in small subsets of premalignant and/or tumor cells in noncancerous chronically inflamed tissues. Genome-wide NGS data also make it possible to clarify the mutational signatures of each cancer tissue by identifying the precise pattern of nucleotide alterations in the cancer genome, providing new information regarding the mechanisms of tumorigenesis. In this review, we highlight these new methods taking advantage of NGS technologies, and discuss our current understanding of carcinogenic mechanisms elucidated from such approaches. View Full-Text
Keywords: mutational signature; deep sequencing; next-generation sequencing; inflammation-associated carcinogenesis; activation-induced cytidine deaminase; AID; APOBEC; transition; transversion; noncancerous tissues mutational signature; deep sequencing; next-generation sequencing; inflammation-associated carcinogenesis; activation-induced cytidine deaminase; AID; APOBEC; transition; transversion; noncancerous tissues
Show Figures

Figure 1

MDPI and ACS Style

Matsumoto, T.; Shimizu, T.; Takai, A.; Marusawa, H. Exploring the Mechanisms of Gastrointestinal Cancer Development Using Deep Sequencing Analysis. Cancers 2015, 7, 1037-1051.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop