Regulation of Let-7a-5p and miR-199a-5p Expression by Akt1 Modulates Prostate Cancer Epithelial-to-Mesenchymal Transition via the Transforming Growth Factor-β Pathway
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture, shRNA Gene Silencing, Antibodies, and Other Reagents
2.2. NanoString-Based miRNA and mRNA Profiling
2.3. In Vivo miRNA Isolation and Quantitative RT-PCR
2.4. miRNA Transfection
2.5. Western Blotting
2.6. mirPath v.3 and dbEMT Analysis
2.7. Migration Assay
2.8. Matrigel® Invasion Assay
2.9. Ingenuity® Pathway Analysis
2.10. cBioportal Genomic Data Analysis of the MSKCC Study
2.11. Statistical Analysis
3. Results
3.1. Akt1 Inhibition Changes the microRNA Expression Profile in PC3 and DU145 Cells
3.2. Human PCa Tissues with a High Gleason Score Express Higher miR-199a-5p and Lower Let-7a-5p Compared to the Low Gleason Score Tissues
3.3. Transfections with miR-199a-5p Inhibitor and/or let-7a-5p Mimic Impairs Migration and Invasion of PC3 and DU145 Cell Migration
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Alwhaibi, A.; Parvathagiri, V.; Verma, A.; Artham, S.; Adil, M.S.; Somanath, P.R. Regulation of Let-7a-5p and miR-199a-5p Expression by Akt1 Modulates Prostate Cancer Epithelial-to-Mesenchymal Transition via the Transforming Growth Factor-β Pathway. Cancers 2022, 14, 1625. https://doi.org/10.3390/cancers14071625
Alwhaibi A, Parvathagiri V, Verma A, Artham S, Adil MS, Somanath PR. Regulation of Let-7a-5p and miR-199a-5p Expression by Akt1 Modulates Prostate Cancer Epithelial-to-Mesenchymal Transition via the Transforming Growth Factor-β Pathway. Cancers. 2022; 14(7):1625. https://doi.org/10.3390/cancers14071625
Chicago/Turabian StyleAlwhaibi, Abdulrahman, Varun Parvathagiri, Arti Verma, Sandeep Artham, Mir S. Adil, and Payaningal R. Somanath. 2022. "Regulation of Let-7a-5p and miR-199a-5p Expression by Akt1 Modulates Prostate Cancer Epithelial-to-Mesenchymal Transition via the Transforming Growth Factor-β Pathway" Cancers 14, no. 7: 1625. https://doi.org/10.3390/cancers14071625