Global Genomic and Proteomic Analysis Identified Critical Pathways Modulated by Proto-Oncogene PELP1 in TNBC
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture and Reagents
2.2. Generation of Model Cell Lines
2.3. Cell Growth, Colony Formation and Western Blotting
2.4. DIA Mass Spectrometry
2.5. RNA Sequencing
2.6. Bioinformatic Analysis
2.7. Statistical Analysis
3. Results
3.1. Global Data-Independent Acquisition Mass Spectrometry (DIA-MS) Identified Unique Pathways Modulated by PELP1
3.2. PELP1 Regulated Proteins in TNBC Cells Play a Critical Role in Ribosome Biogenesis
3.3. Global RNA-Seq Analyses Revealed PELP1 Knockdown Impairs Expression of Critical Regulators of Ribosomal Genes Such as c-Myc
3.4. PELP1 Expression Is Upregulated and Positively Correlates with c-Myc Status and Ribosomal Regulators in TNBC Samples
3.5. TCGA-TNBC Cohort RNA-Seq Data Revealed PELP1 Is Involved in Regulation of c-Myc and Ribosome Biogenesis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liu, Z.; Altwegg, K.A.; Liu, J.; Weintraub, S.T.; Chen, Y.; Lai, Z.; Sareddy, G.R.; Viswanadhapalli, S.; Vadlamudi, R.K. Global Genomic and Proteomic Analysis Identified Critical Pathways Modulated by Proto-Oncogene PELP1 in TNBC. Cancers 2022, 14, 930. https://doi.org/10.3390/cancers14040930
Liu Z, Altwegg KA, Liu J, Weintraub ST, Chen Y, Lai Z, Sareddy GR, Viswanadhapalli S, Vadlamudi RK. Global Genomic and Proteomic Analysis Identified Critical Pathways Modulated by Proto-Oncogene PELP1 in TNBC. Cancers. 2022; 14(4):930. https://doi.org/10.3390/cancers14040930
Chicago/Turabian StyleLiu, Zexuan, Kristin A. Altwegg, Junhao Liu, Susan T. Weintraub, Yidong Chen, Zhao Lai, Gangadhara R. Sareddy, Suryavathi Viswanadhapalli, and Ratna K. Vadlamudi. 2022. "Global Genomic and Proteomic Analysis Identified Critical Pathways Modulated by Proto-Oncogene PELP1 in TNBC" Cancers 14, no. 4: 930. https://doi.org/10.3390/cancers14040930